The invention discloses a natural emulsifier as well as a preparation method and application thereof, and belongs to the technical field of personal care, the prepared natural emulsifier is from vegetable oil, and the natural emulsifier prepared by an enzymolysis method contains 60-85wt% of enzymolysis lecithin, 10-30wt% of glycerol, 10-30wt% of glycerol, 10-30wt% of glycerol and 10-30wt% of glycerol. The natural emulsifier disclosed by the invention has the characteristics of natural source, mildness, no irritation, low chromaticity, light grease smell, low viscosity, stability and capability of increasing transdermal absorption of active matters. The cream can be prepared into a milky white formula, provides soft, moist and light skin feeling, adopts renewable raw materials, is simple in preparation method, stable in performance, low in production cost and beneficial to industrial production, and conforms to the green and environment-friendly trend of the current society.