6results about How to "Meet supply needs" patented technology

The invention discloses recombinant escherichia coli with high yield of N-acetylneuraminic acid and application of the recombinant escherichia coli, and relates to the technical field of biological genetic engineering. The invention relates to a recombinant escherichia coli, which is characterized in that the escherichia coli is taken as a host, and free expression of an N-acetylmannosamine epimerase gene yihS from Streptomyces xiamenensis or an N-acetylmannosamine epimerase gene ce3 from Bacteroides polymorpha and an exogenous N-acetylneuraminic acid lyase gene nano A is carried out; and carrying out recombinant expression on N-acetyl hexosamine 1-kinase nahK, a UDP-N-acetyl glucosamine pyrophosphorylase gene glmU and a UDP-N-acetyl glucosamine-2-epimerase gene neuC in the other synthetic route of the ManNAc. According to the recombinant escherichia coli with high yield of N-acetylneuraminic acid, the yield of N-acetylneuraminic acid can reach 23.08 g / L under a shake flask fermentation condition; a two-stage batch feeding strategy is adopted, the yield of N-acetylneuraminic acid in a 5L fermentation tank reaches 71.25 g / L, the molar conversion rate of GlcNAc reaches up to 57.60%, and the method has the potential of industrial application.

This invention provides a method for the artificial breeding of bullfrogs, including bullfrog selection, pairing, breeding, artificial spawning induction, artificial insemination, fertilized egg hatching, and tadpole rearing. This invention uses precise control of water temperature and quality in the breeding water to stimulate the gonadal development of the bullfrogs. It utilizes artificial spawning induction, insemination, fertilized egg hatching, and seedling rearing techniques to solve the problems of existing bullfrog germplasm degradation and difficulties in artificial breeding. By adding *Lactobacillus rhamnosus* to the feed, the mortality rate of bullfrogs is effectively reduced, the hatching rate of fertilized eggs is high, and the seedling growth rate is fast. This allows for a stable and continuous supply of high-quality bullfrog stock, meeting the effective demand for bullfrog seedlings throughout the year, and providing a technical foundation for the artificial breeding of bullfrogs.

The utility model relates to the technical field of hot water equipment, and discloses modularized fuel gas efficient heat exchange hot water equipment which comprises a protective outer frame, a controller is embedded in the position, close to one side, of the front end of the protective outer frame, a heat exchange pipe is fixedly arranged in the protective outer frame, and the heat exchange pipe comprises a water container. A plurality of connecting strips are fixedly arranged in the water container, and fire tubes are fixedly arranged among the interiors of the connecting strips. According to the utility model, through the structural design of the fire tube and the water container, the layout of the water-in-pipe is realized, the heating area is increased, the residence time of gas in the fire tube is prolonged by the industrial grade stainless steel S-shaped welding fire tube group, the heat energy exchange efficiency is greatly improved, the modularization can be expanded in parallel, the occupied area of a single module is small, and the horizontal installation is supported; the intelligent temperature control system is suitable for various spaces, achieves accurate temperature adjustment and on-demand starting and stopping through intelligent control, is convenient to operate, safe and reliable, and is widely suitable for commercial and civil scenes.

This application provides a method and system for dynamic prediction and decision-making regarding the transportation costs of power materials, belonging to the field of power Internet of Things and intelligent supply chain management technology. The method involves: constructing and dynamically updating a Regional Logistics Capability Index (LCI) covering several administrative units based on multi-source data collected from transportation infrastructure data sources, freight platforms, historical waybill databases, and environmental early warning platforms; collecting shipment information, material characteristics, and route information for the target power material transportation task, and inputting this information, along with the corresponding LCI, into a pre-trained transportation cost prediction model to calculate the predicted transportation cost and confidence interval for the target transportation task; and applying the predicted transportation cost and confidence interval to at least one supply chain decision in intelligent supplier selection, risk control for changes in shipping location, and / or the generation of differentiated inventory strategies. This application improves the accuracy of transportation cost prediction, optimizes supply chain decision-making, enhances resilience, and reduces costs.

The utility model discloses an energy accumulator, which relates to the technical field of fluid energy storage equipment and comprises an energy accumulator body, a piston, a heavy hammer and an air bag, the energy accumulator body comprises an energy accumulator cylinder body; the lower portion of the energy accumulator cylinder body is provided with an oil inlet connector, an oil discharge connector and an oil supply port, and the upper portion of the energy accumulator cylinder body is provided with an oil recovery port. The piston, the heavy hammer and the air bag are arranged in the energy accumulator cylinder body; the heavy hammer is arranged above the piston; an air bag is arranged in a central groove in the lower part of the piston; the piston lifting device comprises a driving unit and a pulley mechanism; the driving unit is connected with the pulley mechanism, and the pulley mechanism is connected with the piston, so that when the piston, the air bag and the heavy hammer act together, the effect of the heavy hammer energy accumulator which assists in increasing the pressure regulating reaction speed through the air bag is achieved; or when the driving unit, the pulley mechanism, the piston, the heavy hammer and the air bag act together, the effect of the heavy hammer energy accumulator which can adjust the output pressure through the driving unit and can assist in improving the pressure adjusting reaction speed through the air bag is achieved.

The invention provides a construction method of a prefabricated yard shaped like a Chinese character'ji 'and a seaport wharf concrete member, and belongs to the technical field of building construction.The construction method comprises the following construction steps that S1, the prefabricated yard shaped like the Chinese character'ji' is built, a land area prefabricated area shaped like a Chinese character'ji 'comprises an exclusive prefabricated pedestal, a storage and maintenance pedestal and a shipping channel, and a sea area adaptive structure shaped like the Chinese character'yue' comprises a leveling base and a base bottom beam; s2, exclusive pedestal prefabricated parts on the two sides; s3, the trolley pushing component is moved to the shipping channel along the transverse track; s4, the concrete member is subjected to rail switching operation, and the transportation direction is switched from the transverse direction to the longitudinal direction; s5, the concrete member is conveyed to the matching structure in the shape of the Chinese character'yue 'in the sea area through the trolley and slides to the ship machine; and S6, when the ship engine is replaced, recombining the shore and the base bottom beam. According to the construction method, through construction of the 'L'-shaped prefabricating yard and the coherent construction process, the prefabricating and shipping efficiency of the seaport wharf concrete members can be improved, different ship machines can be flexibly adapted, the transformation construction period can be shortened, and the adaptation cost can be reduced.