The present invention relates to methods of treatment of cancer by targeting tumor associated macrophages. The inventors investigated specific marker exposed on the surface of the macrophage associated tumor in order to detect and target TAMs. They showed that sideroflexin 3, which is absent in normal macrophage, is expressed by tumors associated macrophage. The relates to a method for identifying tumor-associated macrophage (TAM) in a sample comprising the steps of i) detecting the cell surface expression of CD163, CD68, and SFXN3 markers on the cell population contained in the sample and ii) concluding that the cells expressing CD163, CD68, and SFXN3 markers are the TAMs.