New clean method for producing xylitol and arabinose

A technology for arabinose and clean production, applied in chemical instruments and methods, monosaccharides, sugar derivatives, etc., can solve the problems of high energy consumption, large pollution, and difficult chromatographic separation, etc., and achieve the improvement of xylitol yield, The effect of reducing production cost and shortening fermentation time

Inactive Publication Date: 2009-09-23
张兰波
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all need to undergo hydrolysis, and the hydrolysis temperature is relatively high, and the pollution to the environment is relatively large. Since there are other monosaccharides besides the free L-arabinose from hydrolysis, it is difficult to separate by chromatography, and several steps of chromatography are required. Separation can separate L-arabinose from other monosaccharides, high production cost and low degree of automation
[0004] British patent GB2408262 is also a method for extracting L-arabinose from sugar beet pulp, which requires high alkali extraction. If the control is not proper, in addition to the extraction of arabinan, arabinoxylan and arabinogalactan will also be extracted Affect the purity of subsequent acid hydrolysis of L-arabinose
[0005] The above method consumes a lot of energy and pollutes a lot, and is not suitable for large-scale industrial production
[0006] Japan Unitika Co., Ltd. applied for Chinese invention patent CN01800804.6 and announced a method for treating arabinan, arabin-xylan and arabin-galactan with enzymes, but the method has high enzyme cost and The disadvantage of low efficiency of enzymatic hydrolysis, subsequent separation and purification is more difficult, and it is difficult for industrial production
[0007] Chinese patent CN101372700 describes a method for extracting L-arabinose from xylose mother liquor and biomass acid hydrolyzate, which mainly uses Debaria saccharomyces to ferment xylose mother liquor or hydrolyzate for cultivation to remove miscellaneous suga

Method used

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  • New clean method for producing xylitol and arabinose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] (1) Activation of the original strain

[0052] The original strains are xylitol fermentation strains provided by ATTC or China Microorganism Culture Collection Center, mainly Candida tropicalis. For strains with a conversion rate of 75%-97% to xylitol, the original strains are inoculated in a solid enrichment medium on a slope, the culture temperature is 28°C-32°C, and the culture time is 72 hours-96 hours;

[0053] (2) Expansion of training

[0054] Inoculate the activated strain on the enrichment medium for cultivation, the cultivation temperature is 28°C-32°C, the shaking frequency is 150-220 times / min, and the cultivation time is 24 hours;

[0055] (3) Primary fermentation culture

[0056] The seeds cultivated by shake flask vibration are inoculated into 50 liters of primary fermenter according to the inoculum amount of 5%. -350 rpm, fermentation temperature 28°C-32°C, fermentation time 18-24 hours, no bacteria after microscopic examination, can be transferred to...

Embodiment 2

[0078] (1) Activation of the original strain

[0079] The original strains are xylitol fermentation strains provided by ATTC or China Microorganism Culture Collection Center, mainly Candida tropicalis. For strains with a conversion rate of 75%-97% to xylitol, the original strains are inoculated in a solid enrichment medium on a slope, the culture temperature is 28°C-32°C, and the culture time is 72 hours-96 hours;

[0080] (2) Expansion of training

[0081] Inoculate the activated strain on the enrichment medium for cultivation, the cultivation temperature is 28°C-32°C, the shaking frequency is 150-220 times / min, and the cultivation time is 24 hours;

[0082] (3) Primary fermentation culture

[0083] The seeds cultivated by shake flask vibration are inoculated into 100 liters of primary fermenter according to the inoculum size of 5%. -350 rpm, fermentation temperature 28°C-32°C, fermentation time 18-24 hours, no bacteria after microscopic examination, can be transferred to ...

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Abstract

The invention discloses a new clean method for producing xylitol and arabinose; the method originally utilizes xylose mother liquor as raw material, improves the fermentation process and the separation and purification process by pretreatment of the xylose mother liquor, fermentation, separation of fermented fluid thalli, fermentation pre-treatment, nanofiltration, concentration, chromatographic separation of a simulated moving bed, concentration and crystallization, and the like, shortens the fermentation time, lowers the production cost, greatly reduces the discharge amount of sewage, and increases the conversion rate of xylitol and the yield rate of arabinose; the purity of the xylitol and the arabinose which are obtained by utilizing the method can respectively reach more than 99.9 percent and 99 percent; and the yield rate of xylitol and arabinose is improved greatly, thereby creating an effective clean pollution-free industrial road for the production of xylitol and arabinose.

Description

technical field [0001] The invention discloses a new method for cleanly producing xylitol and arabinose. It is the first to utilize xylose mother liquor as a raw material, through pretreatment of xylose mother liquor, fermentation, ceramic membrane filtration, ultrafiltration, nanofiltration, and simulated moving bed chromatography. Separation, concentration and drying of the crystals. By improving the fermentation process and separation and purification process, the fermentation time is shortened, the production cost is reduced, the discharge of sewage is greatly reduced, the conversion rate of xylitol and the yield of arabinose are enhanced, and the xylitol obtained by the method of the present invention is The purity can reach more than 99.9%, the arabinose can reach 99%, and the yield of xylitol and arabinose has been greatly improved, creating an effective, clean and pollution-free industrialization road for the production of xylitol and arabinose. technical background ...

Claims

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Application Information

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IPC IPC(8): C12P7/18C12P19/02C07C31/18C07C29/74C07H3/02C07H1/08
Inventor 张兰波
Owner 张兰波
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