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Detection method of quinolone drugs, detection reagent kit and application

A detection method, the technology of quinolones, is applied in the preparation of test samples, analysis by making materials undergo chemical reactions, and material analysis by observing the influence of chemical indicators, which can solve the problems that are not suitable for on-site detection and promotion, Complicated operation, poor specificity, etc., to achieve high specificity, simple operation, and low reagent cost

Active Publication Date: 2010-03-17
广东省药品检验所
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0007] At present, for the detection of illegally added quinolones, the Chinese Pharmacopoeia mainly uses liquid chromatography and infrared spectroscopy, and the "Quick Identification Manual of Drugs" uses malonic acid, acetic anhydride; Potassium acid / acetic acid test solution identification method, foreign pharmacopoeia (USP32, BP2009) adopts infrared and thin-layer chromatography, yet the acid / anhydride method in the prior art is complicated to operate, needs water bath, is not suitable for on-the-spot detection and popularization, in addition, iodine Bismuth potassium acetic acid or hydrochloric acid solution is an alkaloid reagent, and most alkaloids can react with this reagent to form orange-red precipitates with poor specificity

Method used

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  • Detection method of quinolone drugs, detection reagent kit and application

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Experimental program
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Effect test

Embodiment 1

[0046] Embodiment 1 specificity test

[0047] This embodiment selects metronidazole reference substance, chloramphenicol standard substance, cefpodoxime axetil reference substance, dexamethasone tablet, salicylic acid reference substance, berberine hydrochloride tablet, ofloxacin reference substance, gatiza Star reference substance, norfloxacin reference substance, ciprofloxacin reference substance and pefloxacin reference substance are used as the samples to be tested, and these eleven samples are tested. The detection methods are divided into the following two types:

[0048] method one:

[0049] (1) The samples to be tested are respectively prepared into solutions with a concentration greater than 1 mg / ml;

[0050] (2) configure 100mg / ml ferric ammonium sulfate solution, which contains 20% sulfuric acid;

[0051] (3) Add a drop of ferric ammonium sulfate solution prepared in step (2) to each sample solution to be tested prepared in step (1), and observe the color developm...

Embodiment 2

[0073] Embodiment 2 minimum detection limit test

[0074] This embodiment selects ofloxacin, lomefloxacin, enoxacin, ciprofloxacin, norfloxacin, levofloxacin, fleroxacin, pefloxacin, pazufloxacin, gatifloxacin, toluenesulfonate Tofloxacin acid and enrofloxacin hydrochloride are used as the samples to be tested, and the serial concentration solutions of these twelve samples are prepared respectively earlier, and the concentrations are: 0.04, 0.09, 0.15, 0.20, 0.25, 0.30, 0.38, 0.76, 1.52 and 3.00mg / ml;

[0075] According to the operation steps of Method 1 in Example 1, one drop of ferric ammonium sulfate solution was added to the serial concentration solutions of each sample to be tested, and the color development results were observed.

[0076] It can be seen from the color development results that the minimum detection limits of the twelve samples to be tested are all 0.20 mg / ml (visible to the naked eye), and the color development results are light orange red to orange red....

Embodiment 3

[0077] Example 3 Detection of Acne Eliminating Essence

[0078] In this example, the anti-acne Wuhen essence in the commercially available anti-acne Wuhen kit is selected as the sample to be tested, and whether it is illegally added with quinolones is tested.

[0079] Samples to be tested: acne-free essence, water, 20ml;

[0080] Chromogen: 0.1mg / ml ferric ammonium sulfate solution (without sulfuric acid);

[0081] Preprocessing:

[0082] (1) Remove 2ml of Acne Wuhen Essence, add 1ml of dichloromethane with a dropper, shake vigorously for 30 seconds, let stand, and collect the lower liquid;

[0083] (2) Add 1ml of 0.05mol / L hydrochloric acid aqueous solution to the above-mentioned lower layer liquid, shake vigorously for 30 seconds, let stand, and collect the upper layer liquid;

[0084] Detection: Add 1 drop of 0.1 mg / ml ferric ammonium sulfate solution to the above upper layer liquid, and it will be orange-red, which is judged as positive, indicating that the Acne Elimina...

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Abstract

The invention discloses a detection method of quinolone drugs, a detection reagent kit and the application; in the method, methylene dichloride and hydrochloric acid are adopted to carry out pretreatment to a sample, ferric ammonium sulfate solution or ferric chloride solution is used for color reaction, according to the color development result, the detection of the quinolone drugs is realized. In the invention, mass samples to be detected are sieved preliminarily by fluorescence viewing under an ultraviolet lamp, the operation is simple and suspected positive samples can be rapidly sorted out, and then high specificity and high sensitivity color development reaction is utilized, so as to lead the detection accurate rate to reach 99 percent. The pretreatment method can eliminate effect ofcomplex and various interfering substances in the sample to be detected, and the concentration of the objective compound in the sample to be detected is improved, thereby being beneficial to observation to color reaction results in a latter period by a naked eye. The detection reagent kit in the invention can be used for detecting quinolone drugs which are mixed in cosmetics, health-care foods, disinfection products or Chinese patent drugs, and detecting the quinolone drugs and identification of the preparation.

Description

technical field [0001] The invention relates to the field of compound detection, in particular to a detection method, detection kit and application of quinolones. Background technique [0002] Quinolones antibacterial drugs (4-quinolones), also known as pyroxic acids or pyridoxic acids, are a relatively new class of synthetic antibacterial drugs. The development of this class of drugs began with nalidixic acid in 1962. Since the advent of flufloxacin, more than 50 kinds have been developed and are under development. [0003] Quinolones antibacterial drugs target the deoxyribonucleic acid (DNA) of bacteria, causing irreversible damage to chromosomes, so that bacterial cells no longer divide, mainly acting on antibacterial drugs against Gram-negative bacteria, and weaker against Gram-positive bacteria (Some varieties have better antibacterial effect on Staphylococcus aureus). [0004] Quinolone antibacterial drugs are divided into first, second, third, and fourth generations...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N1/28G01N1/40
Inventor 黄文静蒋忠军洪建文
Owner 广东省药品检验所
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