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Method and device for producing fuel ethanol by solid state fermentation of fiber raw materials

A cellulosic raw material and solid-state fermentation technology, applied in the field of microbial fermentation, to achieve the effects of reducing production costs, making it easy, and overcoming uneven temperature and humidity distribution of the substrate

Inactive Publication Date: 2013-10-09
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional co-cultivation technology mostly uses the mixed fermentation of Aspergillus niger and Trichoderma reesei to produce compound enzymes. Most of these studies focus on the preparation of high-efficiency cellulase. The mixture is subjected to alcoholic fermentation, and there is no report on the use of multi-species solid-state fermentation technology for one-step degradation of cellulosic substrates to produce ethanol

Method used

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  • Method and device for producing fuel ethanol by solid state fermentation of fiber raw materials
  • Method and device for producing fuel ethanol by solid state fermentation of fiber raw materials
  • Method and device for producing fuel ethanol by solid state fermentation of fiber raw materials

Examples

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Embodiment 1

[0034] The schematic diagram of the process is as figure 1As shown, the bagasse was crushed and sieved to collect raw materials with a particle size of 60 mesh, and then carried out low-temperature alkaline pretreatment to remove most of the lignin components in the raw materials that hinder the degradation of cellulose and hemicellulose, and to improve the growth and metabolism of microorganisms. The substrate provides favorable conditions, and the specific process of alkaline pretreatment is as follows: Weigh 100 g of crushed and sieved bagasse raw material (dry basis), mix it with 0.5 mol / L NaOH solution at a solid-liquid mass ratio of 1:20, and mix it with 0.5 mol / L NaOH solution for 80 Stir the reaction in a water bath at ℃ for 2 hours. After the reaction, separate the solid from the liquid, wash the solid until the pH is neutral, and dry it at 50 ℃ until it has a constant weight (completely anhydrous) and store it as a fermentation raw material; in the bagasse raw materia...

Embodiment 2

[0036] Referring to Example 1, the difference is that the mixed bacteria in the spore suspension are Trichoderma reesei CICC40359 and Penicillium decumbens CICC40361, and the volume ratio of the inoculation of the two is 1:2, and the fermentation reactor after inoculation is placed in a constant temperature and humidity In the box, the fermentation temperature is controlled at 28°C and the air humidity is 92%. After 72 hours of fermentation, the sugar content in the substrate has the maximum value. Samples are added to 30mL sterile water at 30°C and 150rpm to dilute and dissolve for 1 hour. After centrifugation, the total sugar concentration is detected. Transfer the parallel sample mixed substrates (including undegraded substrates, sugars produced by fermentation and effective complex enzymes synthesized by microorganisms in the process of degrading substrates) from the 3d fermentation to a 50mL Erlenmeyer flask, and add 30mL0.2M pH5.0 10% (V / V) activated yeast seed solution w...

Embodiment 3

[0038] With reference to Example 1, the difference is that the fermented cellulosic raw material is rice straw powder, and after it is configured through the solid-state fermentation medium configuration process identical to Example 1, according to 8% (Trichoderma reesei CICC40359: Penicillium decumbens LSM-1 is 1:3) (V / V) inoculum amount inoculated and fermented through the same process. After 3 days of mixed bacteria fermentation, the contents of glucose and xylose in the substrate were 10.035g / L and 6.928g / L respectively, and the total sugar concentration reached 20.209g / L; the highest alcohol concentration reached 5.675g / L after 24 hours of fermentation, which was 39.79% of the theoretical alcohol conversion rate. After the fermentation, the solid and liquid are separated, and the solid residue can be recycled and processed into organic fertilizer or feed.

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Abstract

The invention discloses a method and a device for producing fuel ethanol by solid state fermentation of fiber raw materials. The method comprises the following steps of: coupling enzyme generated by the solid state fermentation of mixed bacteria with ethanol generated by the synchronous fermentation of a substrate, and continuously carrying out enzymolysis on the substrate sufficiently by the effective enzyme synthesized by the microorganism and the metabolism of the microorganism in the process of solid state fermentation to promote cellulose and hemicellulose in the substrate to be effectively degraded; synchronously saccharifying and fermenting a carbon resource matrix to be converted into ethanol sufficiently by a mixture of the high-concentration sugar generated by degrading the substrate, the un-degraded matrix of the sugar and the compound enzyme synchronously synthesized by means of fermentation, wherein the ethanol yield is more than 37% of the theoretical conversion rate. The application of high-cost commercial cellulose can be avoided in the process, and the production cost of the fuel ethanol can be greatly reduced. The solid state tray fermentation device provided by the invention is simple in structure, and easy to manufacture, and the defect of enzyme production by fermenting single bacterial strain and the problems that the matrix in a solid state fermentation reactor is uneven in humiture distribution and is easily infected with infectious microbe and the like can be overcome.

Description

Technical field: [0001] The invention relates to the technical field of microbial fermentation, in particular to a method and device for producing fuel ethanol by solid-state fermentation of cellulosic raw materials. Background technique: [0002] The production of biological enzymes such as cellulase is usually divided into two methods: solid-state fermentation and liquid submerged fermentation. At present, most of the biological enzymes sold on the market are produced by liquid submerged fermentation technology. The fermentation preparation process not only produces a large amount of wastewater, but also requires high power consumption such as aeration and stirring, and the production cost is relatively high. Compared with liquid fermentation, solid-state fermentation technology is a fermentation process in which microorganisms use natural substrates as carbon sources on a solid substrate under the condition of no or almost no free water in the system. It has the advantage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/10C12M1/00C12R1/865C12R1/885C12R1/80
CPCY02E50/10
Inventor 袁振宏刘云云张宇许敬亮何敏超庄新姝
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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