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A kind of electrochemical sensor for detecting uric acid and its preparation and application

An electrochemical and sensor technology, applied in the field of electrochemical analysis, can solve the problems of easy agglomeration, limited graphene research and application, few functional groups, etc., to avoid collapse, low detection limit, and uniform distribution.

Active Publication Date: 2018-07-24
HENGYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although graphene has very excellent physical and chemical properties, it is difficult to interact with other media due to the lack of functional groups on the surface of graphene and its chemical inertness.
In addition, the large surface area of ​​graphene and the existence of a large π-conjugated system make it easy to agglomerate in aqueous solution or polar solvents, which greatly limits the further research and application of graphene.

Method used

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  • A kind of electrochemical sensor for detecting uric acid and its preparation and application
  • A kind of electrochemical sensor for detecting uric acid and its preparation and application
  • A kind of electrochemical sensor for detecting uric acid and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation method of the electrochemical sensor of the present embodiment comprises the following steps:

[0044] (1) Preparation of graphite oxide: prepared by the improved Hummers method, take 23mL concentrated sulfuric acid in a three-necked bottle, add 0.5g graphite powder, 0.5g sodium nitrate, place the three-necked bottle in an ice-water bath, stir and cool at low speed for about After 10 minutes, weigh 3.0g of potassium permanganate and add it slowly several times. After the potassium permanganate is added, continue to stir at a low speed for 30 minutes. Move the three-necked bottle to a warm water bath at 35°C and stir at a low speed for 120 minutes. Add 40mL deionized water to the three-necked bottle, keep a constant speed and slow when adding water, move the three-necked bottle to a 95°C water bath and stir for 30 minutes, add 100mL deionized water, and pour the reaction solution into 10mL 30% HO 2 o 2 In this case, the reaction product was bright yello...

Embodiment 2

[0060] The selectivity of the modified electrode prepared in Example 1 was determined. Interference performance is one of the important indicators to measure the practicability of electrochemical sensors. 10μM uric acid is measured, the allowable measurement error is in the range of ±5%, and the influence of some common metal ions, anions, amino acids and other substances in the human body on the determination of uric acid is tested. It was shown that 100 times the K + 、Na + , V 5+ 、Ba 2+ , Zn 2+ , Ca 2+ 、Cd 2+ , Mg 2+ 、Al 3+ , Cl - , L-Aspartic Acid, L-Leucine, L-Valine, L-Glutamic Acid, L-Leucine, L-Isoleucine, L-Arginine, L-Lysine , urea, glucose, 10 times Cu 2+ , Fe 3+ , Pb 2+ 、 Bi 3+ And epinephrine did not interfere with the determination of uric acid. In organisms, ascorbic acid and dopamine often coexist with uric acid and interfere with the determination of uric acid. Experiments show that the oxidation peak potentials of ascorbic acid, dopamine, and u...

Embodiment 3

[0062] Detection of uric acid in human urine. Take 1.0 mL of urine samples from each of the three persons to be tested and put them in 50 mL volumetric flasks. Take 1.0mL of this diluted sample in the electrolytic cell during the measurement, and use 0.1mol / L H 3 PO 4 -NaH 2 PO 4 Buffer solution (pH 3.0) was diluted to 10 mL, which corresponds to a 500-fold dilution of the original urine sample. Connect the modified electrode prepared in Example 1 to an electrochemical workstation, perform voltammetry scanning, and obtain an electrochemical signal response. Then add a certain amount of uric acid standard substance for recovery determination, the results are shown in Table 1.

[0063] Table 1 Determination results and recoveries of uric acid in urine samples

[0064]

[0065] The formula for calculating the recovery rate is:

[0066] Recovery rate = (m a -m x )÷m s ×100%

[0067] In the formula: m a - measured amount after adding standard substance; m x - the or...

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Abstract

The invention discloses a uric acid detection electrochemical sensor and preparation and application thereof.The electrochemical sensor comprises a glass carbon electrode and a film formed by coating the surface of the glass carbon electrode with electrically reduced graphene oxide and cuprous oxide nano particles.According to the main preparation process of the electrochemical sensor, graphite powder is used as a raw material, graphite oxide is prepared through an improved Hummers method and ultrasonically dispersed in distilled water to obtain graphene oxide sol, then the cuprous oxide nano particles are immobilized on a graphene oxide sheet by ultrasonic action, the surface of the glass carbon electrode is modified with a graphene oxide-cuprous oxide nanocomposite through a dispensing method, and finally the graphene oxide is reduced by a constant potential to obtain the electrically reduced graphene oxide and cuprous oxide nano-composite film.The uric acid detection electrochemical sensor has the advantages of being high in sensitivity, wide in linear range, low in quantity requirement, high in anti-jamming capability, short in detection time, low in cost and the like.

Description

technical field [0001] The invention belongs to the field of electrochemical analysis, in particular to an electrochemical sensor for detecting uric acid and its preparation and application. Background technique [0002] Uric acid (2,4,6 trihydroxypurine) is a metabolite of nucleoprotein and nucleic acid. The normal level of uric acid in healthy adults is 0.15-0.4mmol / L in serum and 250-750mg / L in urine. Excess uric acid in the body is a symptom of many diseases, such as gout, kidney failure and hyperuricemia. In addition, high levels of uric acid in the blood can lead to kidney damage and cardiovascular disease. Clinical medicine has proved that excessive uric acid content in the body can easily cause hyperlipidemia, hypertension, coronary heart disease, diabetes, pulmonary heart disease, etc. Therefore, the quantitative analysis of uric acid in human body fluids is of great significance both in drug control and clinical diagnosis. Currently, methods for detecting uric a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/48G01N27/26
CPCG01N27/26G01N27/30G01N27/48
Inventor 邓培红梁静贺全国
Owner HENGYANG NORMAL UNIV
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