Method for producing microalgae oil

A technology of microalgae oil and microalgae, which is applied in the fields of biotechnology and bioenergy, can solve the problems of using fixation and not involving NOX tolerance, so as to increase solubility, alleviate greenhouse effect and exhaust gas pollution, and improve tolerance and carbon sequestration efficiency

Active Publication Date: 2016-06-29
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

CN102703326A discloses a high CO 2 Tolerance and fixation rate of microalgae and their breeding methods, but the algae strains provided by the patent do not involve the oil content of the algae strains, nor does it involve the NO X Tolerance
Cai Zhuoping et al. In the article "Primary Research on the Growth of Dunaliella Salina and Hymenopsis subcardiae Mixed Culture", under the same inoculation ratio, the mixed culture has higher biomass and algae liquid optical density than that alone, but the article did not involve mixed cultures on CO 2 Take advantage of fixed issues

Method used

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  • Method for producing microalgae oil
  • Method for producing microalgae oil
  • Method for producing microalgae oil

Examples

Experimental program
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Effect test

Embodiment 1

[0019] The preparation of embodiment 1 microalgae seed liquid

[0020] BG11 medium was used for microalgae culture, and the medium formulations are shown in Table 1 and Table 2.

[0021] Table 1BG11 medium

[0022]

[0023] * Composition of A5+Cosolution in Table 2 and Table 1

[0024]

[0025] Scenedesmus obliquus FSH-Y2 and cellulosus SS-B7 were respectively inoculated in BG11 liquid culture solution, and the pH of the BG11 medium for cultivating Scenedesmus obliquus FSH-Y2 was adjusted to 10-12. Cultivate in a constant temperature light shaker, the culture temperature is 25°C, the light cycle is 24h, the light-dark time ratio is 14:10, the light intensity is 5000Lux, 120rpm shaking culture to the logarithmic growth phase, and Scenedesmus obliquus FSH-Y2 is obtained The seed liquid and the cellulosic algae SS-B7 seed liquid are stored at 15°C under low light for future use.

[0026] Inoculate the above-mentioned Scenedesmus obliquus FSH-Y2 seed liquid, cellulosic al...

Embodiment 2

[0027] The preparation of embodiment 2 microalgae oil

[0028] The seed solution prepared in Example 1 was respectively inoculated in BG11 medium to produce microalgae oil. Carried out in an airlift photobioreactor, Scenedesmus obliquus FSH-Y2 seed liquid, cellulosus SS-B7 seed liquid and mixed microalgae seed liquid mixed according to the volume ratio of 3:1, inoculated according to the inoculum size of 10% In the BG11 medium, the pH value is controlled between 9 and 11, and a mixed gas of nitrogen and carbon dioxide is introduced, the carbon dioxide content is 5v% to 45v%, the light intensity is 5000Lux, the culture temperature is 28°C, and the light cycle is 24h , The light-to-dark time ratio is 14:10. After cultivating for 7 days, it is in a stable period. After cultivating, the algae liquid is collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder is measured to calculate the biomass production, which is ...

Embodiment 3

[0032] Embodiment 3 Utilizes flue gas to prepare microalgae oil

[0033] The preparation conditions are the same as in Example 1, except that the feed containing NO, NO 2 and CO 2 flue gas, CO in the flue gas 2 The content is 10v% ~ 30v%, NO and NO 2 The content is 100×10 -6 ~500×10 -6 (v / v), the pH of the reaction system was maintained at 10. After 10 days, the culture was terminated, the algal cells were collected by centrifugation, and the dry weight of the algal powder was measured after vacuum freeze-drying at -60°C to a constant weight, and the biomass production was calculated, and the Normal hexane: ethyl acetate method records total lipid content, and the results are shown in table 4.

[0034] Table 4 The cultivation effect comparison of mixed algae and single algae

[0035]

[0036] It can be seen from Table 4 that compared with single algae, mixed algae can not only tolerate high concentrations of CO 2 , and can tolerate a certain concentration of NOx, and...

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Abstract

The invention discloses a method for producing microalgae oil. The method comprises the following steps: adding a microalgae culture medium and a mixed microalgae seed solution to a photobioreactor, keeping the pH value of a culture system to be 8-12 and introducing gas which contains 5-45v% of CO2 by volume, wherein the mixed microalgae includes scenedesmus obliqnus FSH-Y2 and ankistrodesmus sp. SS-B7 which are preserved in China General Microbiological Culture Collection Center on September 11, 2012 and April 15, 2013 by preservation numbers of CGMCC No.6551 and CGMCC No.7478, respectively. By virtue of the method disclosed by the invention, the solubility and the tolerance of the microalgae culture system to the high-concentration CO2 are improved, a carbon sequestration efficiency is improved and the yield of the microalgae oil is obviously improved, and the obtained microalgae oil can be used for producing biodiesel.

Description

technical field [0001] The invention belongs to the field of biotechnology and bioenergy, in particular to a 2 Method for producing microalgal oil. Background technique [0002] Due to the decrease of fossil energy and the increase of greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is a potential resource for th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64B01D53/84B01D53/62B01D53/60C12R1/89
CPCY02A50/20Y02E50/10Y02P20/151Y02P20/59
Inventor 师文静廖莎姚新武樊亚超孙启梅王领民高大成李晓姝
Owner CHINA PETROLEUM & CHEM CORP
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