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A sodium alginate-modified gelatin-modified dopamine composite scaffold and preparation method thereof

A sodium alginate and composite scaffold technology, which is applied in the preparation of carboxylic acid amides, the preparation of organic compounds, chemical instruments and methods, etc., can solve the problems of not very extensive biological three-dimensional molding and the mismatch of printing speed, and achieve optimized preparation. , The process is simple and convenient, and the effect of exchange and transportation is guaranteed

Active Publication Date: 2021-02-12
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, 3D printing is undoubtedly a technology that people have been keen to study in the past ten years. However, due to the limitation of equipment, the materials suitable for three-dimensional biological modeling are not very extensive, especially hydrogel materials. At present, most of these materials are extruded with thermoplastics. Or rely on environmental stimulus response molding (such as light curing and temperature response), but often the stimulus response of the external environment does not match the printing speed, so this limits the application of materials in 3D printing molding

Method used

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  • A sodium alginate-modified gelatin-modified dopamine composite scaffold and preparation method thereof
  • A sodium alginate-modified gelatin-modified dopamine composite scaffold and preparation method thereof
  • A sodium alginate-modified gelatin-modified dopamine composite scaffold and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] (1) Synthesis of modified gelatin and modified dopamine

[0039] Dissolve 10g of gelatin in 100ml of phosphate buffer (PH=7.4) at 50°C, add 6ml of methacrylic anhydride dropwise, react for 3 hours, transfer to a 14000Da dialysis bag for dialysis, change the water twice a day, after 7 days The spongy substance obtained by freeze-drying for 48 hours is modified gelatin.

[0040] 4g of sodium tetraborate and 1.6g of sodium bicarbonate were dissolved in 40ml of deionized water; under nitrogen protection, 2g of dopamine hydrochloride was added to dissolve; then methacrylic anhydride (1.88ml) dissolved in tetrahydrofuran (10ml) was added dropwise, using 1M sodium hydroxide solution to adjust PH = 9, after reacting for 12 hours, extract 2 times with 20ml ethyl acetate to remove the organic solvent, obtain the lower layer solution, adjust PH = 2 with concentrated hydrochloric acid, then extract 3 times with 50ml ethyl acetate, and then add n-hexane The light yellow powder obta...

Embodiment 2

[0048] (1) Synthesize modified gelatin and modified dopamine according to the method in step (1) of Example 1.

[0049] (2) Preparation of printing paste

[0050] The whole system of printing paste is 20ml of deionized water. Wherein, at normal temperature, 0.03g of anhydrous calcium chloride was dissolved in 10ml of deionized water, and then 1g of sodium alginate was added to obtain a mixed solution a. At 50°C, dissolve 2g of modified gelatin in 10ml of deionized water, add 0.16g of initiator 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and dissolve in advance After stirring and mixing 200 μl of dimethyl sulfoxide and 0.1 g of modified dopamine evenly, stand still to remove air bubbles, then mix and stir the two solutions evenly, and ultrasonicate at 50°C for 30 minutes to obtain a printing paste.

[0051] (3) Three-dimensional printing of sodium alginate-modified gelatin-modified dopamine composite scaffolds containing calcium ions and pre-crosslinking

[0052] Pu...

Embodiment 3

[0056] (1) Synthesize modified gelatin according to the method of step (1) of Example 1.

[0057] (2) Preparation of printing paste

[0058] The whole system of printing paste is 20ml of deionized water. Wherein, at normal temperature, 0.03g of anhydrous calcium chloride was dissolved in 10ml of deionized water, and then 1g of sodium alginate was added to obtain a mixed solution a. Dissolve 2g of modified gelatin in 10ml of deionized water at 50°C, then add 0.16g of initiator 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and mix well , stand still to remove air bubbles, then mix and stir the two solutions evenly, and ultrasonicate at 50° C. for 30 minutes to obtain printing paste.

[0059] (3) Three-dimensional printing of sodium alginate-modified gelatin composite scaffolds containing calcium ions and pre-crosslinking

[0060] Put the printing paste into a three-dimensional printer to print the calcium ion-containing pre-crosslinked sodium alginate-modified gelatin-...

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Abstract

The invention discloses a sodium alginate-modified gelatin-modified dopamine composite scaffold and a preparation method thereof. The preparation method comprises the following steps: double-bond modification of gelatin and dopamine; preparation of three-dimensional printing slurry; and three-dimensional printing and molding of the scaffold for tissue engineering. According to the invention, a small amount of calcium ions are used for pre-crosslinking so as to obtain the slurry beneficial for printing and molding and similar to high-solid-content inorganic powder; the prepared scaffold can maintain its three-dimensional porous structure; preparation of the printing slurry is optimized; printing is carried out without too many supplementary conditions; and materials are greatly saved. The preparation method for the composite scaffold prepared in the invention has the advantages of mild conditions, simple process and easy realization. The prepared composite scaffold can be used in the fields of skin repair, drug sustained-release, tissue engineering, etc.

Description

technical field [0001] The invention belongs to the field of preparation of tissue engineering scaffolds, in particular to a sodium alginate-modified gelatin-modified dopamine composite scaffold and a preparation method thereof. Background technique [0002] Gel is a kind of polymer material containing hygroscopicity, which has attracted the attention of researchers because of its good biocompatibility. Methods for forming hydrogel scaffolds include mold method, phase separation, freeze-drying, foaming method, particle leaching method, and three-dimensional printing. It is the continuous phase and the solvent is the dispersed phase. The porous scaffolds were obtained by freeze-drying. The process of phase separation is relatively complicated, and the shape of the scaffold cannot be precisely controlled. The gas foaming method is to reduce the pressure of the sheet polymer soaked in high-pressure carbon dioxide to the normal state, so that the bubbles increase and nucleate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/60A61L27/20A61L27/22A61L27/56A61K47/36A61K47/42C08H1/00C07C231/02C07C233/20B33Y70/00
CPCA61K47/36A61K47/42A61L27/20A61L27/222A61L27/56A61L27/60A61L2430/34B33Y70/00C07C231/02C08H1/00C07C233/20C08L5/04C08L89/00
Inventor 方立明陈海婷赵亮
Owner SOUTH CHINA UNIV OF TECH