A strain of Lactobacillus plantarum and its application in fermented feed

A technology of Lactobacillus plantarum and feed, applied in animal feed, animal feed, botanical equipment and methods, etc., can solve the problems of short shelf life and limited improvement of fermented feed nutritional value, and achieve extended shelf life, reduced content, Good for storage

Active Publication Date: 2019-11-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, among the existing lactic acid bacteria, there are few strains with both acid-producing ability and bacteriostatic properties. In addition, feed fermentation is usually a mixed bacteria system, and lactic acid bacteria introduced from outside sources can easily break the balance of mixed microorganisms in fermented feed, making the fermented feed The improvement of nutritional value is limited, and the shelf life is not long

Method used

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  • A strain of Lactobacillus plantarum and its application in fermented feed
  • A strain of Lactobacillus plantarum and its application in fermented feed
  • A strain of Lactobacillus plantarum and its application in fermented feed

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Experimental program
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Effect test

Embodiment 1

[0022] 1: In order to achieve a better separation effect, put the fermented soybean meal into the MRS liquid medium according to the inoculum amount of 10g / 100mL, culture at 30°C, 200rpm for 48h, and continuously proliferate for 2 to 3 times.

[0023] The formula of MRS medium is as follows: peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, diamine citrate 2.0g, Tween-80 1.0g, dipotassium hydrogen phosphate 2.0g, magnesium sulfate heptahydrate 0.2g, manganese sulfate heptahydrate 0.05g, calcium carbonate 20.0g, agar 20.0g, distilled water to 1.0L, pH 6.8.

[0024] 2: Separation and purification of strains: Dilute the sample step by step with sterile physiological saline, spread it on the plate of the separation medium (containing 2% calcium carbonate) that has been prepared, and cover it with a layer of the same medium again, Sandwich anaerobic culture (30°C, 48h), and then select a single colony with a large calcium-dissolving circle, s...

Embodiment 2

[0029] 1. Preparation of indicator bacteria suspension: Escherichia coli, Salmonella and Staphylococcus aureus and other pathogenic bacteria glycerol tubes were inoculated in the culture medium, cultured on a shaker at 37°C at 200r / min for 16-18 hours, and the bacterial concentration reached 1.2× 10 10 CFU / mL.

[0030] 2. Preparation of Lactobacillus suspension: Inoculate pure Lactobacillus plantarum into 100mL MRS liquid medium under sterile conditions, and culture at 37°C for 24-48 hours, and the bacterial concentration reaches 1.5×10 10 CFU / mL.

[0031] 3. Preparation of double-layer plates Take a plate with a diameter of about 90 mm, pour 18-20 mL of heated and melted nutrient agar medium into the plate, spread it evenly in the plate, place it on a horizontal platform to solidify, and use it as the bottom layer. Take another semi-solid nutrient agar medium (1% agar content) and heat it to melt in an appropriate amount, let it cool to 48-50°C, add 0.1-0.2mL of indicator b...

Embodiment 3

[0042] Refer to "Berger's Bacteria Identification Handbook" and "Lactic Acid Bacteria Classification and Identification and Experimental Methods", and perform 16sRNA sequencing on strain JUN-DY-6. Among them, the results of the physiological and biochemical experiments of the strains are shown in Table 4, and the results of the carbon source fermentation experiments are shown in Table 3, combining the results of the physiological, biochemical and carbon source fermentation experiments, and comparing the 16S information. The strain was identified as Lactobacillus plantarum according to cell microscopic morphology, physiological and biochemical data and 16S rRNA gene sequence data.

[0043] Table 3: Sugar fermentation test results of Lactobacillus plantarum JUN-DY-6.

[0044]

[0045] Note: "+" means strong fermentation, "d" means slight fermentation, "-" means no fermentation

[0046] Table 4: Physicochemical test results of Lactobacillus plantarum JUN-DY-6.

[0047]

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Abstract

The invention discloses a plant lactobacillus and its application in fermented feed, belonging to the technical field of fermented feed. Lactobacillus plantarum (Lactobacillus plantarum) JUN-DY-6 of the present invention has been preserved in the China Typical Culture Collection Center on March 23, 2017, with a preservation number of CCTCC M 2017138, and the preservation address is China, Wuhan, Wuhan University. The bacterial strain of the present invention has a wide acid spectrum, high acid production, broad-spectrum antibacterial effect, can effectively reduce the content of anti-nutritional factors in fermented soybean meal, reduce the incidence of diarrhea, and improve the utilization level of nutrients. The bacterial strain is used to prepare feed It can effectively prolong the storage period of feed.

Description

technical field [0001] The invention relates to a strain of lactobacillus plantarum and its application in fermented feed, belonging to the technical field of fermented feed. Background technique [0002] With the rapid development of the aquaculture industry, since the 1950s, in order to increase animal production, aquaculture enterprises have added antibiotics to animal feed, which has made great contributions to the development of the entire aquaculture industry. But as time goes by, antibiotics are used more and more frequently, and the harm is becoming more and more obvious, which has aroused great concern from people from all walks of life. At present, the concept that feed safety is equivalent to food safety has become a consensus in the world. Studies have found that fermented feed can inhibit the growth of pathogenic bacteria in the digestive tract and play a certain role in preventing animal diseases. At the same time, it also has the advantages of good palatabili...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A23L33/135A23K10/18A23K10/12A61K35/747C12R1/25
CPCA01N63/00A61K35/747A23K10/12A23K10/18A23L33/135C12N1/205C12R2001/25Y02A50/30
Inventor 邓禹邹宗胜赵运英毛银
Owner JIANGNAN UNIV
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