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Application of Callicarpa nudiflora extract in preparation of lipid-lowering drugs

A kind of technology of nudibranch and extract, which is applied in the field of natural medicine to achieve the effect of enhancing metabolic capacity, preventing obesity and reducing liver damage

Active Publication Date: 2019-10-15
深圳市南芮环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to comprehensive literature reports, the chemical components found in Auranthus nudica are mainly flavonoids, phenylpropanoids, iridoids and triterpenoids; the naked pharmacological activities mainly include hemostasis, anti-inflammation, antibacterial, improving immunity, anti-bacteria, etc. Tumor and other effects; overall, the activity of the crude extract of Auranthus nudica is more obvious than that of monomer compounds, and the research scope is wider, but there is no information about its lipid-lowering and weight-loss and improvement of related metabolic diseases (hyperlipidemia, non-alcoholic fat liver and insulin resistance, etc.)

Method used

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  • Application of Callicarpa nudiflora extract in preparation of lipid-lowering drugs
  • Application of Callicarpa nudiflora extract in preparation of lipid-lowering drugs
  • Application of Callicarpa nudiflora extract in preparation of lipid-lowering drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 naked flower purple pearl extract

[0025] Nudiflora extract (LHZZ) is prepared by the following method: take freshly dried Nadiflora leaves, air-dry them naturally, crush them through an 80-mesh sieve, and powder Mix with methanol, ultrasonically extract at 40°C for 30min, cool with ice water, shake up and take 1.5mL of the extract, freeze and centrifuge at 10°C, 12000rpm for 10min, let it stand for 1-2min, take 500μL of the supernatant and pass through a 0.22μm micro pore filter membrane, and the filtrate was freeze-dried to obtain the extract of Auranthus nudica.

Embodiment 2

[0026] Example 2 The in vitro lipid-lowering effect of Auranthus nudiflora extract on adipocytes

[0027] 1.1 Reagents and instruments

[0028] Fetal bovine serum (Serana), penicillin, streptomycin, DMEM medium, insulin, isobutylmethylxanthine (Sigma), dexamethasone (Sigma), cell counting kit-8 (ccK-8) (Jiancheng Bioengineering Institute, Nanjing, China), electronic balance (Sartorius), chloroform, isopropanol, absolute ethanol, QPCR reverse transcription kit, PCR instrument (Bio-rid) fluorescence quantitative PCR instrument (Thermo Fisher, USA)

[0029] 1.2 Cell culture and drug treatment

[0030] (1) Cell culture: Mouse 3T3-L1 (CL-173, ATCC) preadipocytes were supplemented with 10% v / v newborn calf serum (Serana), penicillin G (100IU / mL) and streptomycin ( 100 μg / mL) in DMEM. Cells were treated with an adipogenic mixture consisting of 10 μg / mL insulin, 500 μM isobutylmethylxanthine (Sigma) and 1 μM dexamethasone (Sigma) in the presence of 10% v / v fetal bovine serum (FBS) ...

Embodiment 3

[0040] Example 3 In vivo lipid-lowering and weight-reducing effect of Nadihua Zizhu extract

[0041] 1.1 Reagents and instruments

[0042] Gastrointestinal needles, feed, bedding, blood glucose test strips, blood glucose meter (ACON BIOTECH, China), thermometer, ice maker, -80 ℃ ultra-low temperature refrigerator, automatic biochemical analyzer (Hitachi 7080, Japan)

[0043] 1.2 Animal modeling, group administration, fasting blood glucose and fasting glucose tolerance test

[0044] (1) Animal modeling and group administration: 6-week-old male wild-type C57BL / 6J (WT) mice were purchased from Guangzhou University of Traditional Chinese Medicine (Guangzhou, China). Mice were maintained in a temperature-controlled animal room at 20-22°C with a 12-h light / dark cycle. Half of the C57BL / 6 mice were fed with normal diet (9% fat; laboratory diet), the other half were fed with HFD (60kcal% fat; Research Diets, USA), and had free access to water until the end of the experiment. Three m...

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Abstract

The invention discloses application of a Callicarpa nudiflora extract in preparation of lipid-lowering drugs. The extract is prepared by naturally air-drying fresh dry leaves of Callicarpa nudiflora,crushing the leaves, sieving the crushed leaves, performing ultrasonic extraction with methanol, cooling, freezing and centrifuging an extract liquid, allowing a product to stand, treating a liquid supernatant with a millipore filter membrane, and freeze-drying the filtrate to obtain the extract. It is proved that the extract has obvious lipid-lowering and slimming effects in in-vitro tests and in-vivo tests of mice, can be used for preparing lipid-lowering drugs, can relieve symptoms of obesity-related metabolic diseases (hyperlipidemia, non-alcoholic fatty liver disease, insulin resistance,and the like), and has a good application prospect, high safety, and no cytotoxic effect.

Description

technical field [0001] The invention belongs to the field of natural medicines, and in particular relates to the application of the extract of Auranthus nudiflora in the preparation of lipid-lowering medicines. Background technique [0002] Obesity is a manifestation of energy metabolism disorders in the body, and is the main risk factor for a series of diseases such as type 2 diabetes, non-alcoholic fatty liver, cardiovascular disease, senile dementia, and even tumors, which seriously threatens the health of all human beings. According to World Health Organization estimates, there are more than 1 billion people who are overweight (BMI>25), and the number of typical obese patients exceeds 300 million (BMI>30); in the United States, more than two-thirds of adults are experiencing Overweight or obesity; in China, high-calorie diet represented by high fat, high sugar and high protein, fast-paced work and sedentary, the obese population is gradually increasing, according t...

Claims

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Application Information

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IPC IPC(8): A61K36/85A61P3/06A61P1/16A61P29/00A61P3/04
CPCA61K36/85A61P3/06A61P1/16A61P29/00A61P3/04Y02A50/30
Inventor 陶琳
Owner 深圳市南芮环保科技有限公司
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