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Method for purification of D-pinitol from carob bean water extract

A technology for water extract and pinitol, which is applied in the field of D-pinitol purification, can solve the problems of poor purification effect, high sugar content, high impurity content, etc., so as to improve the practical degree and development value, and improve the food safety. , the effect of high conversion

Active Publication Date: 2020-03-06
ZHEJIANG JIANFENG HEALTH TECH
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Problems solved by technology

Moreover, it has been found through research that in the Middle East carob water extract, the D-pinitol content is about 5-6%, but the water content is about 39%, the fructose content is about 13%, the glucose content is about 13%, and the sucrose content is about 22.3%. There are many impurities, and for diabetics and other people who are not suitable for sugar intake, the proportion of sugar is too high, and the content of blood sugar-raising factors fructose, sucrose, and glucose is large, which can easily lead to blood sugar rise, so reducing the impurities in the extract content, while purifying D-pinitol, reducing the amount of blood sugar-raising factors is an urgent problem to be solved
[0006] At present, the methods of extracting D-pinitol are commonly used solvent method and ultrasonic method, after extraction, ion exchange resin, precipitant, etc. are used to purify, but these methods have poor purification effect, high impurity content, and almost no blood sugar raising factor. removed

Method used

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  • Method for purification of D-pinitol from carob bean water extract
  • Method for purification of D-pinitol from carob bean water extract
  • Method for purification of D-pinitol from carob bean water extract

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Experimental program
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Effect test

Embodiment 1

[0040] Embodiment 1: D-pinitol purification one

[0041] (1) Raw material pretreatment: Weigh 1kg (about 1000ml) raw material solution carob water extract, then take 0.5L distilled water for dilution, put the diluted raw material solution in the reactor, stir evenly, and then adjust the pH with acetic acid =4-5, stir evenly; in the carob water extract raw material liquid that the present embodiment adopts, contain about 6% of D-pinitol, about 13% of fructose, about 13% of glucose content, about 22.3% of sucrose content, after calculation The raw material liquid contains about 60g of D-pinitol, about 130g of fructose, about 130g of glucose, about 223g of sucrose, and the rest is impurities and water;

[0042] (2) Hydrolysis conversion of sucrose: heat the pretreated raw material liquid water in a water bath until the temperature of the raw material liquid is about 55°C, add 150 mg of invertase, heat-preserve and hydrolyze and stir for 3 hours to obtain a hydrolyzate, and the st...

Embodiment 2

[0047] Embodiment 2: D-pinitol purification two

[0048] Before the purification of D-pinitol, fructose adsorbent needs to be prepared, and the preparation steps are as follows:

[0049] Volcanic stone activation pretreatment: clean 200g of volcanic stone, dry it in an oven at 70°C for 12 hours, then calcinate at 480±5°C for 2 hours, grind it after cooling, and pass through a 100-mesh sieve to obtain activated volcanic stone;

[0050] Volcanic rock removal: add the activated volcanic rock to 15wt% citric acid solution, stir and soak for 10 hours, then clean it with distilled water, then immerse it in 2wt% sodium hydroxide solution, stir and soak it for 8 hours, and then clean it with distilled water. Air-dried; the consumption of citric acid solution and sodium hydroxide solution is to cover the volcanic rock;

[0051] Calcium ions loaded on volcanic stone: Add 10g of ethylenediaminetetraacetic acid into deionized water, stir to dissolve, then add the volcanic stone after rem...

Embodiment 1

[0060] Detecting Example 1: chromatographic conditions and system suitability test

[0061] Chromatographic column: Agilent sugar analysis column ZORBAX Carbohydrate4.6×250, 5um, flow rate: 1.0ml / min, column temperature: 35°C, water A: acetonitrile B is listed as the mobile phase according to the gradient list, the specific parameters are shown in Table 1; Light scattering detector detection, drift tube temperature: 41°C, air pressure 350-355KPa. The number of theoretical plates should not be less than 2500 based on the peak of bilobalide.

[0062] Table 1

[0063] time, min A，％ B，％ 0 8 92 20 8 92 25 20 80 40 20 80 42 8 92 45 8 92

[0064] Preparation of control chart: Weigh four reference substances of D-pinitol, fructose, glucose and sucrose respectively, and add water to make a solution with a concentration of 2.0mg / 1ml.

[0065] Preparation of the sample solution: Measure 2.0 g of the corresponding solution in the four...

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Abstract

Belonging to the technical field of D-pinitol purification, the invention discloses a method for purification of D-pinitol from a carob bean water extract. The method for purification of D-pinitol includes the steps of: raw material pretreatment: adjusting the pH value of a raw material solution to acidic; sucrose hydrolysis conversion: adding sucrase into the raw material solution, and hydrolyzing and converting sucrose into fructose and glucose; fructose precipitation: removing fructose by precipitation method; glucose oxidation: oxidizing glucose into gluconic acid; and purification of D-pinitol: separating gluconic acid by chromatography, and conducting reduced pressure concentration on eluent to obtain high-purity D-pinitol. The invention aims to use a mild purification method to reduce impurities and remove blood sugar raising factors at the same time as many as possible step by step.

Description

technical field [0001] The invention relates to the technical field of D-pinitol purification, in particular to a method for purifying D-pinitol from carob water extract. Background technique [0002] D-pinitol (D-pinitol), IUPC is (1S, 2S, 4S, 5R)-6-methoxycyclo hexane.1, 2, 3, 4, 5.pentol, CAS registration number is 10284-63-6, Chinese The aliases are dextro-inositol methyl ether, 3-methyl inositol (mateze), keninol, methyl inositol, pinitol, behenyl alcohol, and the English aliases are Sennite, L-chiro-Inositol, pinite, pinecamphor , the molecular formula is C 7 h 140 o 6 , the molecular weight is 194.18, D-pinitol is a white granular crystal in methanol, odorless, sweet, soluble in water, insoluble in methanol, ethanol, insoluble in chloroform, acetone, ether and other organic solvents. The melting point of D-pinitol is 186-188°C; the specific rotation is 65.5. Its structure is as follows: [0003] [0004] D-pinitol is a substance with many important physiologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C41/36C07C43/196C12P19/14C12P19/02
CPCC07C41/36C12P19/14C12P19/02C07C2601/14C07C43/196
Inventor 李文德杨浩蒲首丞赵虎军楼永增
Owner ZHEJIANG JIANFENG HEALTH TECH
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