Usnic acid photodegradation product and application thereof in preparation of drugs, cosmetics or health-care products resistant to ultraviolet radiation
A technology of photodegradation and usnic acid, applied in the field of biomedicine, can solve problems such as the biological activity of usnic acid photodegradation which is not seen
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Examples
Embodiment 1
[0036] Example 1 Analysis and Identification of the Chemical Component Structure of Usnenic Acid Photodegradation Compound
[0037] 1. Materials and instruments
[0038] Analytical grade dichloromethane, chloroform, cyclohexane, and acetone were purchased from Sinopharm Reagent Co., Ltd. Chromatographic grade acetonitrile and methanol were purchased from Thermo Fisher (USA). (+)-Usnic acid standard (>98%) was purchased from Sigma-Aldrich (Shanghai). Usnic acid was identified by Professor Wang Changhong from the Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine with a purity >98%. The drug strong light irradiation test box is provided by Chongqing Yongsheng Instrument. The UV energy measuring instrument (model UV-int150) was produced by UV-Design in Germany and purchased from Dongguan Youtu Instrument Equipment Co., Ltd. 1D- and 2D-NMR spectra were measured with a Bruker AVance-III (400M-600MHz) NMR spectrometer. With tetramethylsilan...
Embodiment 2
[0067] Example 2 Evaluation of Usnic Acid and Photodegradation Compounds on Human Liver L02 Cell Toxicity and Ultraviolet Radiation Protection
[0068] 1. Materials and instruments
[0069] Human normal liver cell line L02 (Cell Bank, Chinese Academy of Sciences); RPMI-1640 medium (Gibco, USA), fetal bovine serum (FBS), penicillin / keymycin (Gibco, USA), PBS (Gibco, USA); MTT (Sigma, USA); Hoechst33342 / PI kit (Nanjing Jiancheng Institute of Bioengineering, China).
[0070] BioTek Synergy H4 microplate reader (BioTek, USA); CO 2 Constant temperature incubator, biological safety cabinet (Thermo, USA); centrifuge, pipette gun (Eppendorf, Germany); fluorescence inverted microscope (Olympus, Japan); ultrapure water filter (Millipore, USA); culture dish, centrifuge tube (Corning, USA); 48-well culture plate, 96-well culture plate (Costar, USA); 1.5ml EP tube, 5.0ml EP tube (Sinopharm Reagent Co., Ltd.); 10μl pipette tip, 200μl pipette tip, 1 ml pipette tips (Crystalgen, USA).
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Embodiment 3
[0101] Example 3 Study on epidermal melanocyte cytotoxicity and anti-ultraviolet radiation effect of usnic acid and its photodegradation compound
[0102] 1. Materials and instruments
[0103] Human epidermal melanin iCell-016 primary cells (Subcomb (Shanghai) Biotechnology Co., Ltd., China); human melanocyte complete medium (Subcomb (Shanghai) Biotechnology Co., Ltd., China); BeyoColor TM Color pre-stained protein molecular weight standard (Beiyuntian Biotechnology, China); BCA protein concentration determination kit, SDS sodium dodecyl sulfate, RIPA lysate, PMSF (Beijing Aidelai Biotechnology Co., Ltd., China); glycine, Tris (Dalian Meilun Biotechnology Co., Ltd., China); Clarity TM Western ECL Subatrate (BIORAD, USA); Immobilon-P SQ PVDF membrane (Millipore, USA); PAGE gel rapid preparation kit (Shanghai Yazyme Biotechnology Co., Ltd., China); Anti-Tyrosinase Polyclonal primary antibody (Bioss, USA); Anti-beta-ActinMonoclonal primary antibody, HRP Conjugated Goat anti-...
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Abstract
Description
Claims
Application Information
- IPC
- A61K31/343; A61K31/216; A61K31/222; A61K31/12; A61P39/00; A61P17/00; A61K8/49; A61K8/37; A61K8/35; A61Q17/04; C07C67/00; C07C69/738; C07C69/21; A23L33/10
- CPC
- A61K31/343; A61K31/216; A61K31/222; A61K31/12; A61P39/00; A61P17/00; C07C69/738; C07C69/21
- Inventors
- 王长虹; 邝淑芬