Degradation method of livestock and poultry manure antibiotics

A technology of livestock and poultry manure and antibiotics, applied in the field of degradation of livestock and poultry manure antibiotics, can solve the problems of long time, difficulty in using fermentation residue as fertilizer, and high cost of antibiotics, and achieve efficient and simple effects, which are conducive to crop growth and safe raw materials non-toxic effect

Inactive Publication Date: 2020-09-08
BEIJING ZIGUANG YINGLI CHEM TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there are safety hazards in the use of livestock and poultry manure, urine and fermentation waste residue from antibiotic enterprises, and there is no safe and efficient treatment method, which leads to the prohibition of livestock and poultry manure as fertilizer, and the use of fermentation waste residue as fertilizer is difficult
[0003] In the prior art, sodium percarbonate, potassium percarbonate or calcium percarbonate are generally used for oxidative degradation of feces, or hydrogen peroxide and some additives are used for oxidative degradation of feces, but the degradation effect is not good, and sodium percarbonate is used to degrade the feces. The cost of antibiotics is relatively high, the time is relatively long, and it is difficult to carry out effective degradation reaction at room temperature, so it is necessary to develop a more efficient, economical and convenient method for the degradation of antibiotics in feces

Method used

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  • Degradation method of livestock and poultry manure antibiotics
  • Degradation method of livestock and poultry manure antibiotics
  • Degradation method of livestock and poultry manure antibiotics

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1: The experiment of degrading tetracycline with the degradation reagent containing hydrogen peroxide

[0032] S1: Prepare 4.3mg / l tetracycline solution, take 1ml and add degradation reagent in a beaker. The composition and amount of degradation reagent are shown in Table 1 below.

[0033] S2: After completing the above steps, perform degradation according to the reaction temperature and reaction time in Table 1, and the degradation reaction is carried out under the stirring condition of a magnetic stirrer.

[0034] S3: After completing the above steps, ultrasonically vibrate for 10-20 minutes, then centrifuge at 3500-4500r / min for 5-10 minutes, get the supernatant and measure, and measure the tetracycline content by ELISA at 450mm with a microplate reader.

[0035] As shown in Table 1, add 1 ml of reagents to 1 ml of tetracycline solution, add 1 ml of distilled water to the blank group numbered 1, and add 1 ml of reagents in numbers 2-9. For the ingredients in...

Embodiment 2

[0039] Embodiment 2: the experiment of different concentrations of hydrogen peroxide degrading livestock and poultry feces antibiotics

[0040] S1: Collect 20g of chicken manure fermented to a water content of 20%wt, add tetracycline solution, and adjust the initial concentration of tetracycline in the manure to be 500ppm.

[0041] S2: Add sodium carbonate-hydrogen peroxide mixed reagent to chicken manure, and react at room temperature for 2 hours. Sodium carbonate in the sodium carbonate-hydrogen peroxide mixed reagent is in a saturated state (mass concentration is 18.1%), and the concentration of hydrogen peroxide is 30wt%.

[0042] S3: The supernatant was taken for ultrasonic vibration for 20 minutes, then centrifuged at 4500 r / min for 10 minutes, and the supernatant was taken for determination. The content of tetracycline was determined by ELISA method with a microplate reader at 450 mm.

[0043] Table 2 Experimental conditions and results of antibiotic degradation in ch...

Embodiment 3

[0046] Embodiment 3: the experiment of different addition amount of sodium carbonate-hydrogen peroxide mixed reagent degrades antibiotic

[0047] S1: Collect 20g of chicken manure fermented to a water content of 10%wt, add tetracycline solution, and adjust the initial concentration of tetracycline in the manure to be 250ppm.

[0048] S2: Add sodium carbonate-hydrogen peroxide mixed reagent to chicken manure, and react at room temperature for 2 hours. Sodium carbonate in the sodium carbonate-hydrogen peroxide mixed reagent is in a saturated state (mass concentration is 18.1%), and the concentration of hydrogen peroxide is 30wt%.

[0049] S3: Add 0.1M hydrochloric acid:methanol (50ml:50ml) extractant after completion of the reaction to extract antibiotics, add extractant according to the amount of extractant:chicken manure=2:1 (v / m).

[0050] S4: The supernatant was taken for ultrasonic vibration for 20 minutes, then centrifuged at 4500 r / min for 10 minutes, and the supernatant...

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Abstract

The invention relates to a degradation method of livestock and poultry manure antibiotics in particular to a method for producing a safe organic fertilizer by disinfecting livestock and poultry manureat normal temperature and effectively removing antibiotics. The method comprises the following steps: mixing an alkali carbonate-hydrogen peroxide mixed reagent in livestock and poultry manure, or firstly mixing hydrogen peroxide and then mixing an alkali carbonate solution, and standing or mixing and stirring for more than 1 hour so as to obtain the organic fertilizer with the antibiotic contentof less than 10ppb. According to the method, greener and safer carbonate peroxide is ingeniously utilized to destroy the antibiotic structure in the livestock and poultry manure, the activity and thetoxicity of antibiotics are reduced, and germs in the manure are sterilized and inactivated. The simple process does not cause secondary pollution to the environment, the antibiotic content of the treated livestock and poultry manure can be reduced from 4300ppb to about 10ppb and is far lower than the antibiotic content requirement of 750ppb, and the degradation rate is 99.77%; the content of bacteria can be reduced from 9.2 billion/g to below 30,000/g, and the sterilization rate can reach 99.997%. Use is safer, and the potassium carbonate/hydrogen peroxide compound is more suitable for beingused as fertilizer.

Description

technical field [0001] The invention belongs to the field of organic fertilizer production, in particular to a method for effectively degrading antibiotics in livestock and poultry manure and biomass and simultaneously normal temperature sterilization to produce safe organic fertilizers, in particular to a method for degrading antibiotics in livestock and poultry manure. Background technique [0002] With the development of intensive farming, the use of veterinary antibiotics is becoming more and more common. However, antibiotics cannot be completely absorbed and degraded by animals, and most of them are still excreted in feces and urine in the form of prototypes or metabolites, resulting in excessive antibiotics in livestock and poultry manure. On the other hand, there are still a large amount of antibiotics that need to be effectively treated in the fermentation waste residue of antibiotic production enterprises. At present, antibiotics widely used in livestock and poultr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F3/00C05G3/00C05G3/60A01N59/00A01P1/00A01P3/00
CPCA01N59/00C05D1/00C05D9/00C05F3/00C05G3/00C05G3/60Y02A40/20
Inventor 曹皓彭云贵尹应武尹海超
Owner BEIJING ZIGUANG YINGLI CHEM TECH CO LTD
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