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Biocontrol plant endophytic bacterium ZN-S2 and application thereof

A ZN-S2, phytopathogenic bacteria technology, applied in the directions of plant growth regulators, applications, bacteria, etc., can solve the problems of affecting the effect of chemical control, high input cost, long cycle, etc., to improve product quality and sustainable, reduce Expenditure, Compatible Effects

Pending Publication Date: 2021-04-23
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, breeding is limited due to the long cycle and high input cost; chemical control is fast, cheap and efficient, but currently effective agents for bacterial diseases mainly include: copper preparations, zinc preparations, antibiotics, thiamethoxam, etc. pesticide
However, the frequent use of chemical pesticides and the characteristics of rapid genetic variation and high fitness of bacterial reproduction make pathogenic bacteria quickly develop resistance, which affects the control effect of pesticides

Method used

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  • Biocontrol plant endophytic bacterium ZN-S2 and application thereof
  • Biocontrol plant endophytic bacterium ZN-S2 and application thereof
  • Biocontrol plant endophytic bacterium ZN-S2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, isolation culture and identification of bacillus (Bacillus amyloliquefaciens) bacterial strain:

[0036] Beef extract peptone medium (NA): add distilled water to 1000 mL in 5 g of tryptone, 3 g of beef extract, 1 g of yeast powder, 10 g of D-glucose agar, and 15 g of agar; , sterilized at 121°C for 20 minutes).

[0037] Without adding agar, it is NB culture medium.

[0038] The bacillus described in the present invention can be obtained by isolating and culturing in the laboratory according to the following conditions.

[0039]Collect and cultivate Dendrobium candidum from Zhejiang, rinse the collected Dendrobium with tap water, sterilize the rhizome of Dendrobium in 75% (v / v) alcohol solution for 30 seconds, then sterilize in 1% (v / v) sodium hypochlorite solution for 10 minutes, Rinse with sterile water 3 times. Cut the roots and stems with sterile scissors, and grind them with a sterile mortar, suspend the plant powder with an appropriate amount of st...

Embodiment 2

[0041] Embodiment 2, identification of bacterial strain ZN-S2:

[0042] The Bacillus amyloliquefaciens ZN-S2 (i.e. Bacillus amyloliquefaciens ZN-S2 CGMCCNO.18962) of the present invention has the following characteristics: the suitable growth temperature of the bacterial species on the NA plate is 37° C.; the colony is white, the surface is dry, and the diameter after 24 hours of cultivation is 0.5 After 36 hours of culture, white spores appeared, the colony was round in shape, but the border was irregular, the bacteria were rod-shaped, and Gram staining was positive. Morphology such as figure 1 shown.

[0043] The extracted ZN-S2 genomic DNA was subjected to 16S rRNA sequencing to obtain a gene sequence with a full length of 1397bp. Homology comparison was performed on BLAST in NCBI, and the results showed that ZN-S2 was more similar to Bacillus sp. high. Using MEGA version 6.0 software, the Maximum Likelihood method was used for genetic distance analysis, and the phylogen...

Embodiment 3

[0045] Embodiment 3, the antagonism of bacillus ZN-S2 to different crop pathogenic bacteria ( image 3 , Figure 4 ):

[0046] (1) Preparation of crop pathogen flat plate: prepare NA flat plate according to the method described in Example 1, pour the prepared medium into a sterile petri dish (Φ9cm) in an ultra-clean workbench, and make a NA flat plate with a thickness of about 5mm . Erwinia (cabbage soft rot, sweet potato stem rot, pear blight), Xanthomonas (rice bacterial blight, rice thin strip disease, broccoli black rot) activated in the culture medium in advance, Pseudomonas (cucumber bacterial angular spot, pine cauliflower bulb rot, broccoli bulb rot), Laueria (tomato solanacear) and other pathogenic bacteria, pipette 200 μl of pathogenic bacteria respectively solution (activated bacterial solution in NB culture solution) was dripped into a sterilized petri dish, and poured into 20mL of NA medium cooled to 40°C-50°C, spread the petri dish in one layer, and shake gent...

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Abstract

The invention discloses bacillus amyloliquefaciens ZN-S2, and the preservation number of the bacillus amyloliquefaciens ZN-S2 is CGMCCNO:18962. The invention further provides application of the bacillus amyloliquefaciens ZN-S2 at the same time, wherein the bacillus amyloliquefaciens ZN-S2 is used for inhibiting the growth of plant pathogenic bacteria, so that the plant bacterial diseases are prevented and controlled.

Description

technical field [0001] The invention belongs to the technical field of crop disease prevention and control, and in particular relates to an endophytic bacterium ZN-S2 for biocontrol plants and its application in bacterial control of economic crops. Background technique [0002] Bacterial diseases of crops caused by Ralstonia sp., Ervinia sp., Xanthomonas sp. or Pseudomonas sp. are important in economic crop production. Major diseases, which cause heavy losses every year, and in severe cases can lead to failure of single-season crops. Such pathogenic bacteria can spread in the field through rainwater, seeds, plant residues or agricultural operations, and invade plants through plant stomata, wounds, etc., which can quickly cause soft rot and withering of plants, resulting in damage to plant organs and loss of function. In the later stage of some pathogenic bacteria infection, water drop-shaped bacterial pus, which is clearly visible to the naked eye, can be produced on the su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01G13/00A01G22/22A01G22/05C12R1/07
CPCA01N63/22A01G13/00A01G22/22A01G22/05
Inventor 吴慧明饶琼张传清
Owner ZHEJIANG FORESTRY UNIVERSITY
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