Preparation method of mackerel antioxidant peptide and application of mackerel antioxidant peptide in facial mask

An anti-oxidant peptide and mackerel technology, which is applied in cosmetic preparations, skin care preparations, cosmetics, etc., can solve the problems of low resource utilization and under-exploited economic value, so as to strengthen defense capabilities and repair damaged skin. Effect of damaging cells and repairing damaged skin

Pending Publication Date: 2021-09-21
QUANZHOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a high-protein economic fish, mackerel is mainly used for direct consumption, the

Method used

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  • Preparation method of mackerel antioxidant peptide and application of mackerel antioxidant peptide in facial mask
  • Preparation method of mackerel antioxidant peptide and application of mackerel antioxidant peptide in facial mask
  • Preparation method of mackerel antioxidant peptide and application of mackerel antioxidant peptide in facial mask

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of embodiment 1 mackerel antioxidant peptide

[0034] Take minced mackerel meat, add deionized water to the minced mackerel meat according to the solid-to-liquid ratio of 1mg: 3-5mL, to obtain a minced meat solution; heat the minced meat solution in a boiling water bath for 15-20 minutes, and naturally cool to room temperature . Then, adjust the pH of the solution to 8.5, add alkaline protease according to the standard of 3000U / g minced meat, and enzymolyze in a 50°C water bath with stirring (80-100r / min) for 2h. After the enzymolysis, the solution was placed in a water bath at 100° C. for 10 minutes, and then the solution was centrifuged (8000 r / min for 20 minutes) to remove the precipitate, and the supernatant was freeze-dried to obtain a mackerel antioxidant peptide dry powder product.

[0035] The molecular weight distribution of the obtained mackerel antioxidant peptides is shown in Table 1 and figure 1 shown. The active peptides prepared by the ...

Embodiment 2

[0038] Example 2 Antioxidant Efficacy Evaluation of Mackerel Antioxidant Peptides

[0039] The obtained mackerel polypeptide was formulated into peptide solutions with different concentrations with deionized water, and its ability to scavenge DPPH and hydroxyl free radicals was analyzed to evaluate the antioxidant capacity of the mackerel active polypeptide.

[0040] (1) Detection of DPPH scavenging ability, reference: Zhang Ling. The cultivation and active substances of Chlorella sorokiniana C74[D]. Haikou: Hainan University, 2015.

[0041] Take 3 centrifuge tubes and mark them as A 0 、A i and A j . to A 0 Add 1mL DPPH solution and 1mL absolute ethanol to tube Ai, add 1mL DPPH solution + 1mL sample to be tested into Ai tube, j Add 1mL of the sample to be tested and 1mL of absolute ethanol. OD517 was measured using a spectrophotometer. The clearance rate is calculated according to the following formula:

[0042]DPPH clearance rate (%) = (A 0 -A i +A j ) / A 0 ×100

...

Embodiment 3

[0047] Example 3 Production of a whitening and moisturizing mask containing mackerel antioxidant peptide

[0048] A whitening and moisturizing mask containing mackerel antioxidant peptide, which is composed of the following ingredients: 0.3% macromolecule sodium hyaluronate, 0.3% small molecule sodium hyaluronate, 0.3% wrinkled carrageen powder, 2.0% betaine, Allantoin 0.2%, Trehalose 2.0%, Dextran 0.5%, (Fish) Hydrolyzed Collagen Peptide 0.3%, Mackerel Antioxidant Peptide 0.3%, Oligopeptide-1 0.3%, Glycerin 5.0%, Water Soluble Olive Oil 0.3%, 1,2-pentanediol 0.3%, propylene glycol 0.2%, sorbitol 0.1%, fragrance 0.04%, hydrogenated castor oil (PEG-40) 0.15%, phenoxyethanol 0.2%, vitamin C 0.5%, niacinamide 0.2%, and the balance is made up with pure water.

[0049] The preparation method comprises the following steps:

[0050] 1) Weigh 0.3 parts of small molecule sodium hyaluronate and 0.3 parts of macromolecular sodium hyaluronate powder and dissolve them in a small amount o...

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Abstract

The invention discloses a preparation method of a mackerel antioxidant peptide and application of the mackerel antioxidant peptide in a facial mask, the method comprises the following steps: adding water into mackerel meat paste, stirring to obtain a meat paste solution, heating in a boiling water bath for 15-20 minutes, and cooling; and performing enzymolysis for 1.5-3.5 hours by using alkaline protease, performing water bath for 10 minutes at the temperature of 100 DEG C after the enzymolysis is finished, performing centrifugation to remove precipitates, and performing freeze drying on supernate to obtain the mackerel antioxidant peptide. The mackerel antioxidant peptide for preparing a whitening and moisturizing facial mask, and the mackerel antioxidant peptide with good antioxidant activity is added into the mask, so that various free radicals can be effectively removed, cell metabolism can be promoted, and damaged cells of skin can be repaired; meanwhile, the facial mask is rich in sodium hyaluronate, trehalose, glucan and the like, can lock facial skin moisture, keeps the skin moist, and has the effects of resisting wrinkles and beautifying; and the combined use of the antioxidant peptide, the vitamin C and the nicotinamide can also achieve a whitening effect.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a preparation method of mackerel antioxidant peptide and its application in facial masks. Background technique [0002] Bioactive peptides have various biological activities such as anti-oxidation and antibacterial, and their activities are determined by molecular size, amino acid type and sequence. Bioactive peptides not only have the advantages of low toxicity, high efficiency, and high selectivity, but also have good water solubility, low viscosity, low foaming rate, and low gelation, so they have great potential in the fields of health food, medicine, and cosmetics. Wide application prospects. Among them, bioactive peptides with antioxidant function are also called antioxidant peptides, which can improve the oxidation of unsaturated fatty acids and prevent adverse consequences such as protein crosslinking, DNA denaturation, and membrane damage in biological ti...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K8/64A61K8/65A61K8/73A61K8/60A61K8/44A61K8/49A61Q19/08
CPCC12P21/06A61K8/64A61K8/65A61K8/73A61K8/60A61K8/735A61K8/44A61K8/4946A61Q19/08
Inventor 王宝贝刘璐璐杨槟煌陈洪彬郑宗平
Owner QUANZHOU NORMAL UNIV
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