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Method for producing complex microbial inoculant through fermentation of clostridium butyricum centrifugal waste liquid, corresponding complex microbial inoculant and application of complex microbial inoculant

The technology of a compound bacterial agent and clostridium butyricum is applied in the field of microbial bacterial agent and aquaculture, which can solve the problems of increasing the burden on enterprises and environmental protection, small market demand, difficult preservation, etc., and achieves stable production and good convenience, Improve the industrial chain, the effect of not easy to contaminate bacteria

Pending Publication Date: 2021-11-05
山东益昊生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the small market demand for Clostridium butyricum centrifugate, it is not easy to store, and it is easy to stain and stink, so it can only be sent to sewage treatment plants as waste liquid, which increases the burden on enterprises and environmental protection

Method used

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  • Method for producing complex microbial inoculant through fermentation of clostridium butyricum centrifugal waste liquid, corresponding complex microbial inoculant and application of complex microbial inoculant
  • Method for producing complex microbial inoculant through fermentation of clostridium butyricum centrifugal waste liquid, corresponding complex microbial inoculant and application of complex microbial inoculant
  • Method for producing complex microbial inoculant through fermentation of clostridium butyricum centrifugal waste liquid, corresponding complex microbial inoculant and application of complex microbial inoculant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The present invention utilizes Clostridium butyricum centrifuge waste liquid and fermentation bacterial agent to jointly produce and prepare a kind of composite bacterial agent, the screening process of the specific operation parameter condition in the production process is as follows:

[0034] 1. Addition ratio of Clostridium butyricum centrifugate

[0035] The frozen Clostridium butyricum was inoculated in RCM liquid medium, anaerobically cultured at 37°C until the cell production was over 90%, the fermentation broth was centrifuged at high speed, and the sludge was sent to the next process to make Clostridium butyricum powder. Collect the centrifugate, add water and 4% brown sugar, 0.01% magnesium sulfate, 0.01% manganese sulfate, 0.03% potassium dihydrogen phosphate, 0.02% acetic acid in different proportions (0%, 25%, 50% and 75%) Sodium, adjust pH to 7.0, add compound fermentation agent (0.1% of Bacillus subtilis, 0.1% of Bacillus licheniformis, 0.05% of lactic ac...

Embodiment 2

[0081] Embodiment 2: the water purification effect of composite bacterial agent in aquaculture water body

[0082] 1. Add the compound bacterial agent to the aquaculture water with ammonia nitrogen and pH exceeding the standard at a rate of 5 kg / mu, add the compound bacterial agent every three days, take samples, and test the pH value and ammonia nitrogen in the water.

[0083] The results are shown in Table 9. The pH index of the aquaculture water returned to normal after the compound bacterial agent was added on the third day, and the ammonia nitrogen index tended to normal on the sixth day.

[0084] Table 9: Changes in water body indicators after adding compound bacterial agents

[0085]

[0086] 2. Add the compound bacterial agent at a ratio of 3kg / mu to the aquaculture water body with normal indicators (this water body had a bloom in the same period last year), add the compound bacterial agent every 7 days, and take samples to detect the pH value and ammonia nitrogen i...

Embodiment 3

[0090] Embodiment 3: the effect of composite bacterial agent in aquatic animal breeding

[0091] 1. Add the compound bacterial agent to the feed at an amount of 50 ml / kg, and feed Penaeus vannamei; at the same time, set up a control group, only feed the feed without adding any compound bacterial agent. Penaeus vannamei was cultured according to the normal feeding mode. The volume of a single group of water tanks was 1m×1m×1m, and 80 prawns were cultured. After 14 days of continuous feeding, the length and survival rate of the two groups of prawns were detected.

[0092] The results are shown in Table 11. Compared with the control group, after 14 days of feeding, the Penaeus vannamei in the compound bacterial agent group obviously grew faster and had a higher survival rate.

[0093] Table 11: Effects of compound bacterial agents on the growth of Penaeus vannamei

[0094]

[0095] 2. Add the compound bacterial agent to the feed at the dosage of 50ml / kg, and feed the grass c...

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Abstract

The invention discloses a method for producing a complex microbial inoculant through fermentation of clostridium butyricum centrifugal waste liquid, the corresponding complex microbial inoculant and application of the complex microbial inoculant. The production method comprises the following steps: adding clear water into the clostridium butyricum centrifugal waste liquid for dilution, respectively adding a carbon source and inorganic salt into the diluent, adjusting the pH to be neutral, heating to 60-80 DEG C, and sterilizing for 15-30 minutes; and jointly putting the disinfected clostridium butyricum centrifugal waste liquid, bacillus subtilis powder, saccharomycetes powder and lactic acid bacteria powder into a fermentation tank, co-culturing for 15-25 h in two stages, and obtaining the complex microbial inoculant. The complex microbial inoculant not only can reduce ammonia nitrogen in aquatic water and purify water, but also can increase the weight gain rate and the survival rate of aquatic animals, and not only can turn clostridium butyricum centrifugal waste liquid into wealth and relieve the environmental protection burden, but also can create income for enterprises.

Description

technical field [0001] The invention belongs to the field of microbial bacterial agents and aquaculture, and in particular relates to a method for producing composite bacterial agents by fermentation of Clostridium butyricum centrifuge waste liquid and the corresponding composite bacterial agents and applications. Background technique [0002] Clostridium butyricum ( Clostridium butyricum ) belongs to the Bacillus family, the genus Clostridium, Gram-positive, with spores, spores oval, eccentric or subterminal, resistant to adverse environments, and has a strong intestine-regulating effect, which can inhibit pathogenic bacteria in the intestinal tract , Promote the growth of beneficial bacteria in the intestines such as Bifidobacteria and Lactobacilli. Clostridium butyricum centrifugate is a by-product of the production of Clostridium butyricum, in which protein, polysaccharides, inorganic salts and other nutrients are very rich, not only contains more live bacteria of Clost...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C02F3/34A23K50/80A23K10/18C12R1/125C12R1/145C12R1/01C12R1/645C02F103/20
CPCC12N1/20C12N1/14C02F3/34A23K50/80A23K10/18C02F2103/20
Inventor 高素芹李昊彦张明俊王晓伟武传菊赵志强孙海燕杨庆利
Owner 山东益昊生物科技有限公司
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