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Composite microbial agent containing bacillus subtilis and bacillus licheniformis and application thereof

A compound microbial inoculant, Bacillus licheniformis technology, applied in the directions of microorganisms, microbial treatment, microorganism-based methods, etc., can solve the problems of low sterilization effect, affecting food safety, soil pollution, etc., and achieve easy storage and transportation. time, the effect of promoting growth and reproduction

Pending Publication Date: 2022-06-03
春华秋实科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, there are a variety of fungicides on the market, mainly used to kill various pathogenic microorganisms, mainly to reduce the occurrence of crop diseases and insect pests. The amount of bactericidal drug used will increase year by year, resulting in poor bactericidal effect, high dosage, strong drug damage, poor drug resistance, high cost of drug use, and serious soil pollution
[0003] Most of the existing fungicides use chemical reagents. The abuse of chemical preparations will produce certain drug residues on crops, seriously affecting food safety, or the fungicides that appear have single ingredients and low bactericidal effects; Most of them contain a single biologically active ingredient, a single microbial strain or a single traditional Chinese medicine fungicide, which has poor antibacterial effect and high production cost

Method used

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  • Composite microbial agent containing bacillus subtilis and bacillus licheniformis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation of a nano-zero-valent iron feather protein is provided: under nitrogen protection, 4 g of feather protein is dissolved in 100 mL of a 70% ethanol aqueous solution, then 4 mL of a 5% sodium persulfate aqueous solution is added, and stirring is initiated for 5 minutes; 20 mL of methyl diallyl ammonium chloride solution and 16 mL of remaining sodium persulfate solution were added dropwise to the induced feather protein solution, then 10 mL of nano-zero valent iron suspension was added, and the reaction was continued for 4 h under reflux conditions. The ethanol was removed by rotary evaporation, the pH was adjusted to 13, the supernatant was taken by centrifugation, and freeze-dried to obtain nano zero-valent iron feather protein;

[0023] The suspension of the nano-zero-valent iron is subjected to a liquid-phase reduction method, and in a liquid-phase system protected by nitrogen, the nano-zero-valent iron is obtained by reducing ferrous sulfate with potassi...

Embodiment 2

[0025] Provide a preparation method of Bacillus subtilis bacterial suspension: inoculate Bacillus subtilis seed liquid in PDA liquid medium, cultivate at 28 DEG C for 20 hours, then inoculate 3% of the inoculation amount on the fermentation medium, and inoculate it on the fermentation medium at 30° C. Cultivate at ℃ for 48 hours to obtain Bacillus subtilis bacterial suspension. The fermentation medium consists of sterilized glucose 1%, peptone 1%, yeast powder 0.5%, magnesium sulfate 0.15%, potassium dihydrogen phosphate 0.15%, and the balance The water composition, pH adjusted to 6.8-7.2.

[0026] A method for preparing a Bacillus licheniformis bacterial suspension is provided: inoculate the Bacillus licheniformis seed liquid on a glucose peptone medium, cultivate at 28° C. for 20 hours, and then inoculate the fermentation medium with 3% of the inoculum amount, and at 30° C. Cultivate at ℃ for 48h to obtain Bacillus licheniformis bacterial suspension. The fermentation medium ...

Embodiment 3

[0028] A composite microbial inoculum containing Bacillus subtilis and Bacillus licheniformis, comprising the following raw materials in parts by weight: 3 parts of nano-zero-valent iron feather protein, 7 parts of duck manure, 20 parts of soybean straw, 10 parts of horse manure, shrimp shells 15 parts of powder, 10 parts of urea, 15 parts of diammonium hydrogen phosphate, 6 parts of potassium chloride, 20 parts of microbial bacterial suspension, 5 parts of adjuvant; the microbial bacterial suspension includes Bacillus subtilis bacterial suspension and Bacillus licheniformis Bacterial suspension, the volume ratio of the two is 20:7; the adjuvant is a mixture of corn starch, sodium hydroxymethyl cellulose, polyvinylpyrrolidone; the feather protein is obtained from poultry including but not limited to chickens, ducks, and geese. Get it.

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Abstract

The invention discloses a compound microbial agent containing bacillus subtilis and bacillus licheniformis. Comprising the following raw materials in parts by weight: 3-5 parts of nano zero-valent iron feather protein, 7-9 parts of duck manure, 20-25 parts of soybean straw, 10-15 parts of horse manure, 15-20 parts of shrimp shell powder, 10-12 parts of urea, 15-18 parts of diammonium hydrogen phosphate, 6-8 parts of potassium chloride, 20-40 parts of a microbial suspension and 5-8 parts of an auxiliary agent. The microbial suspension comprises a bacillus subtilis suspension and a bacillus licheniformis suspension, and the volume ratio of the bacillus subtilis suspension to the bacillus licheniformis suspension is (20: 7)-(11: 13); the number of effective viable bacteria in the compound microbial agent is not less than 0.2 billion / g. The prepared compound microbial agent has a good prevention and treatment effect on fruit and vegetable root nematodes, is long in action time, can promote the growth and reproduction of plants, is easy to store and transport, and is low in inactivation rate.

Description

technical field [0001] The invention relates to the technical field of compound microorganisms, in particular to a compound microorganism inoculum containing Bacillus subtilis and Bacillus licheniformis and its application. Background technique [0002] At present, there are a dazzling array of fungicides on the market, which are mainly used to kill various pathogenic microorganisms, mainly to reduce the production of crop diseases and insect pests. The amount of bactericidal drugs used will increase year by year, resulting in poor bactericidal effect, high dosage, strong phytotoxicity, poor drug resistance, high drug cost and serious soil pollution. [0003] Most of the existing fungicides use chemical reagents. The abuse of chemical preparations will produce certain drug residues on crops, which will seriously affect food safety, or the fungicides that appear have a single component and low bactericidal effect; the biological fungicides currently on the market, Most of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/20C05G3/40C05G3/60C05G5/12C12R1/125C12R1/10
CPCC12N1/20C05B7/00C05F17/20C05G3/40C05G3/60C05G5/12C05C9/00C05C11/00C05D9/02C05F1/005C05F3/00C05F11/00Y02W30/40
Inventor 祝春华祝博伟祝伟倩李佩佩
Owner 春华秋实科技集团有限公司
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