The invention discloses durable water transfer printing tattoo ink. The durable water transfer printing tattoo ink comprises the following components: color dye, glycerinum, propylene glycol and water. The invention also discloses a tattoo sticker using the durable water transfer printing tattoo ink as well as a use method thereof. According to the tattoo ink and the tattoo sticker which are disclosed by the invention, to-be-printed patterns can be formed through the existing printer transfer printing ink, the patterns can be changed as one wishes, and the tattoo sticker is simple to prepare,convenient to use and easy to operation; pigment, a solvent and an additive in the tattoo ink components can serve as medical chemical reagents or natural reagents and are harmless to a human body; and the tattoo can go deep into skin deeply, is absorbed by epidermis, is long in retention time and is not liable to be eliminated or blurred by the conventional solvents such as water, sweat and alcohol.