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Bacillus subtilis HL-1 and application thereof in respect of soil phosphate dissolving

A technology of Bacillus subtilis and inoculum, applied in the application, soil conditioning materials, bacteria and other directions, can solve the problems of less than 20% utilization rate in the current season, waste of resources, etc., and achieves the advantages of promoting recycling, saving resources and simple preparation process. Effect

Active Publication Date: 2012-04-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of traditional chemical fertilizers can alleviate the lack of phosphorus in the soil to a certain extent, but as the main varieties of phosphate fertilizers in China, such as superphosphate and ammonium phosphate, no matter in the calcareous soil in the north or the acidic soil in the south, due to the strong phosphorus fixation effect of the soil , the quarterly utilization rate is less than 20%, resulting in waste of resources
However, it has not been reported that guava residue, a renewable resource, is used as a bio-fertilizer adsorbent material to replace the commonly used non-renewable resource to produce a bacterial agent with high phosphorus-decomposing effect.

Method used

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  • Bacillus subtilis HL-1 and application thereof in respect of soil phosphate dissolving
  • Bacillus subtilis HL-1 and application thereof in respect of soil phosphate dissolving
  • Bacillus subtilis HL-1 and application thereof in respect of soil phosphate dissolving

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation, purification and identification of Bacillus subtilis HL-1

[0037] (1) Separation

[0038] Prepare insoluble inorganic phosphorus medium: glucose 10.0g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, ferrous sulfate heptahydrate 0.03g, manganese sulfate tetrahydrate 0.03g, yeast extract 0.4g, phosphoric acid Calcium 5g, distilled water 1L, pH value 7.0~7.5.

[0039] Take the soil collected on Huolu Mountain, Tianhe District, Guangzhou City, Guangdong Province as the screening soil sample, accurately weigh 10.0g of the soil sample to be tested by the flat plate coating method, and put it into a 250mL triangular flask filled with 90mL sterile water (with glass beads) , shake the shaker for 30 minutes to fully disperse the microorganisms, let it stand for 20~30s, and take the supernatant for 10-fold decreasing dilution, using 10 3 ~10 5 Dilution: Use a pipette gun to pipette 0.1mL of the dilution respectively, spread it on the i...

Embodiment 2

[0043] Example 2 Characterization

[0044] (1) Morphological characteristics of bacteria

[0045] Gram-positive bacteria with spores that do not expand after spore formation. The bacteria are rod-shaped, non-capsulated, motile, and aerobic. Gram staining effect see figure 1 , the cells were stained blue-purple, and the spores were not colored. Spore staining effect see figure 2 , the thallus is yellow-brown, and the spores are light blue.

[0046] (2) Colony Morphological Characteristics

[0047] The colonies formed on the nutrient agar medium were round, yellowish, rough and opaque, with irregular edges.

[0048] (3) Physiological and biochemical characteristics

[0049] According to the Biolog microbial identification instrument, the utilization of 31 different carbon sources by this strain under aerobic conditions was analyzed, and the results are shown in Table 1.

[0050] Features result Features result water + Methyl D-glucoside + ...

Embodiment 3

[0054] Growth characteristics of embodiment 3 HL-1 bacteria under different carbon source environments

[0055] Beef extract peptone medium: 5g beef extract, 5g peptone, 5g sodium chloride, 1000ml water, pH 7.1~7.4.

[0056] Corn flour, wheat bran, glucose, peanut bran, sucrose, rice bran and plant starch were used as carbon sources, respectively, with a concentration of 20 g / L, to replace beef extract in the beef extract-peptone medium, and other components remained unchanged. Boil the above seven kinds of medium for 10 minutes, cool down to room temperature naturally, adjust the pH value to 7.1~7.5 with 1mol / L NaOH or HCl, and prepare the fermentation medium. In 250mL Erlenmeyer flasks, 45mL of each group's culture medium was dispensed, sterilized, and each group's culture medium was repeated three times. According to the inoculation amount of 10%, HL-1 bacteria were inoculated into the above-mentioned sterilized fermentation medium, cultured at a temperature of 33°C and a ...

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Abstract

The invention discloses a bacillus subtilis HL-1 and an application thereof in the respect of soil phosphate dissolving. The strain is collected in China General Microbiological Culture Collection Center (CGMCC) on Aug 24th, 2011, with a collection number of CGMCC No.5175. The 16SrDNA nucleotide sequence of the strain is represented by SEQ ID NO:1. With added diatomite and guava slag adsorbing agent materials, a fermentation broth of the bacillus subtilis HL-1 can be prepared into microbial manure. With the strain provided by the invention, phosphate availability and phosphate fertilizer utilization rate of the soil can be improved, plant growth can be promoted, and soil microbe diversity can be improved. The strain has important significance in reducing chemical fertilizer application, modifying soil micro-ecology, and promoting soil sustainable utilization. The strain has wide application prospect in production practice.

Description

technical field [0001] The present invention relates to a kind of microorganism and application thereof, be specifically related to a kind of Bacillus subtilis ( Bacillus subtilis ) HL-1 and its application in decomposing insoluble phosphorus in soil, improving soil micro-ecological structure and promoting plant growth. Background technique [0002] Phosphorus is one of the nutrient elements necessary for plant growth and development, and has important effects on nitrogen fixation, photosynthesis, energy transfer, signal transduction, biomacromolecule synthesis and respiration. There are 5.7 billion hm in the world 2 The soil is deficient in phosphorus, and the phosphorus concentration in most soils ranges from 0.1 to 10 μmol / L. Under ideal conditions, the phosphorus concentration required for the growth of gramineous plants is 1-5 μmol / L, and the phosphorus concentration required for high phosphorus-demanding plants such as tomatoes and peas is 5~60μmol / L. When phosphoru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C05F11/08C09K17/40C12R1/125C09K101/00
Inventor 李永涛蔡燕飞孙礼勇王亚君赵肃清孔德颖黄娟陈昊
Owner SOUTH CHINA AGRI UNIV
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