Preparation method of composite peptide-copper chelate by utilizing meat processing byproduct

Abstract

The invention provides a preparation production method of a composite peptide-copper chelate by utilizing a meat processing byproduct and a corresponding product composite peptide-copper chelate. The preparation method of the composite peptide-copper chelate comprises the following steps: carrying out enzymolysis on the meat processing byproduct (blood powder, feather meal and the like) with proteolytic enzyme in a certain condition to obtain enzymatic hydrolysate with small peptide as a main component; carrying out a chelation reaction on a copper salt solution and the enzymatic hydrolysate serving as ligand, and controlling the temperature, pH and reaction time of a reaction system so as to prepare a better chelated product through a two-step chelation reaction by adopting a method of determining a charge rate according to the copper ion coordination characteristics and small peptide detection result for the first time. The product composite peptide-copper chelate can be effectively absorbed by animals including ruminant, livestock and aquatic animals, so that the application range of byproducts can be enlarged, and the utilization rate of the byproducts can be improved. The preparation method is less in investment, and is easy to produce on large scale.

Description

technical field [0001] The invention relates to a production method of a novel animal feed additive, in particular to a composite peptide copper chelate and a production method thereof, belonging to the field of animal feed additives. Background technique [0002] As one of the essential trace elements for animals, copper is a component and activator of many enzymes in animals, participates in hair growth and pigmentation, maintains animal reproductive function, regulates the body's neurohumoral mechanism and immune response; at the same time, copper is also a constituent of hemoglobin One of the components of iron affects the hematopoietic function of the body's iron through the blue copper protein, and the production of red blood cells must have the presence of copper. Therefore, if there is a lack of copper, there will be pathological phenomena such as anemia, movement disorders, and abnormal bone metabolism, which will affect the nutrient metabolism and growth and develo...

AI Technical Summary

Problems solved by technology

There are many disadvantages in these two types of products in application: one is poor stability, and cannot be completely absorbed due to the antagonism of the pH value in the body, inorganic ions, organic macromolecules, etc.; Destroy the activity of vitamins and micro-ecology in the feed, and affect the digestion and absorption rate; third, animals absorb less, and most of them are excreted from the body, polluting soil and water sources, and are more destructive to the environment; fourth, a large amount of copper is deposited in the liver of animals , it is very easy to deposit after human consumption, and even cause poisoning, becoming a source of danger for food safety

The third-generation copper element additive is a single amino acid chelate. Although it has the advantages of strong biological stability and easy digestion and absorption, the effect is not significant in practical applications. This is because the animal body needs a variety of organic chelates. The demand for a single specific chelate is small, adding too much will not only easily cause competition for absorption channels, affect the absorption and utilization of other amino acids and copper elements, but also cause waste

At present, the main way of utilization is to prepare feed protein blood cell powder, feather powder, etc. through simple processing, but animals have low digestion and absorption rate of these animal protein feeds, and the feeding value is not high; and my country stipulates that animal protein feeds are not allowed to be used For ruminants, which limits the scope of application of the product

At present, there have been reports on the use of meat processing by-products for the preparation of complex peptide trace element chelates, but the alkaline hydrolysis method or the first alkaline hydrolysis followed by enzymatic hydrolysis treatment process will not only destroy the amino acid activity in the product, but also reduce the Nutritional value, and the requirements for production equipment are high, and the waste liquid produced is relatively polluting to the environment. Therefore, it is imperative to develop a mild and efficient production process for copper complex peptide chelates to comprehensively utilize the by-products of meat processing.