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Preparation method of multifunctional live microorganism preparation for forage

A living bacteria preparation and microbial technology, applied in the field of animal husbandry, can solve the problems of low bioavailability, easy pollution of the environment, and high toxicity of selenite ions, and achieve easy large-scale production and use, low environmental pollution, and improved active effect

Inactive Publication Date: 2013-01-09
江西昌丰由由生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low bioavailability, high toxicity and easy pollution of the environment, the use of selenite ion in suckling pig feed has been restricted in Sweden, and its addition in animal feed has been completely banned in Japan.

Method used

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  • Preparation method of multifunctional live microorganism preparation for forage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Seed pure culture

[0046] (1) Pure-bred culture of Saccharomyces cerevisiae and Candida utilis

[0047] Activation of strains

[0048] Under sterile conditions, take the freeze-dried strains of Saccharomyces cerevisiae or Candida utilis, inoculate them on the slant of malt juice agar in test tubes, and incubate at 30°C for 18-36 hours. When colonies are visible to the naked eye on the slant, it is ready to transplant .

[0049] primary seed culture

[0050] Under sterile conditions, use an inoculation loop to pick a single colony, insert it into a triangular flask filled with seed culture solution, and incubate at 30°C for 18 to 48 hours. grade seeds. The seed medium consisted of 12Brix. wort.

[0051] secondary seed culture

[0052] Under sterile conditions, the secondary seed solution was inserted into a 50 L fermenter with an inoculation volume of 10% by volume, and the filling coefficient was 60% (v / v). The secondary seed medium was: glucose 30 g...

Embodiment 2

[0065] Example 2: Mixed culture of yeast, spores and lactic acid bacteria

[0066] Strain activation and seed culture

[0067] Activation and seed culture of S. cerevisiae, Candida utilis, B. subtilis, B. licheniformis and Lactobacillus casei were the same as described in Example 1.

[0068] Fermentation culture

[0069] The secondary seed liquid of Saccharomyces cerevisiae, Candida utilis, Bacillus subtilis, Bacillus licheniformis and Lactobacillus casei is pressed by Saccharomyces cerevisiae: Candida utilis: Bacillus subtilis: Bacillus licheniformis: Lactobacillus casei=2:2: Inoculate at a ratio of 1:1:1, with a volume ratio of 20% of the total amount of inoculation into 0.5 m 3 In the fermenter, the liquid filling capacity of the fermenter is 70% (v / v).

[0070] The composition of the fermentation medium is: brown sugar 80g / L, corn steep liquor 20g / L, ammonium sulfate 5g / L, magnesium sulfate 2g / L, potassium dihydrogen phosphate 5g / L, dipotassium hydrogen phosphate 5g / L...

Embodiment 3

[0072] Analysis of Bacteria Count and Functional Nutrient Components During Fermentation

[0073] 1. Colony counting:

[0074]Add 500 ppm of copper sulfate to the complete solid medium to inhibit the growth of yeast, and use the dilution plate counting method to distinguish the bacterium and lactic acid bacteria colonies, and determine the total number of lactic acid bacteria and the total number of bacterium.

[0075] Add 500 ppm of ampicillin to the improved yeast solid medium (YPD) to eliminate the interference of the growth of lactic acid bacteria and spores on the detection of the total number of yeasts, and use the dilution plate counting method to determine the total activity of yeasts in fermented products bacteria count.

[0076] 2. Determination method of inorganic selenium and organic selenium:

[0077] Qualitative analysis of inorganic selenium and organic selenium: Take 5~10mL of sample solution (containing more than 50 nmol / L selenium) in a test tube, add 1mL o...

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Abstract

The invention discloses a preparation method of multifunctional live microorganism preparation for forage. The multifunctional live microorganism preparation for forage is rich in organic selenium and glutathione and is made by culturing bacterium mixture in specific medium and adding sodium selenite in the process of fermentation at 28 DEG C to 35 DEG C for 36-48 hours, wherein the bacterium mixture is of two or more of brewer's yeast, candida utilis, bacillus subtilis, bacillus licheniformis and lactobacillus casei. The multifunctional live microorganism preparation for forage has the functions of conventional live microorganism preparations, such as regulating microecological balance of animal intestines and enhancing body immunity and stress resistance. In addition, the multifunctional live microorganism preparation for forage is rich in organic selenium and glutathione and has the advantages of high selenium utilization rate, low toxicity, capability of enhancing animal immunity and the like, and benefit in livestock breeding can be increased effectively.

Description

technical field [0001] The scope of application of the present invention relates to the field of animal husbandry, in particular to a multifunctional live microorganism preparation for feeding. On the one hand, it has the functions of conventional microbial living bacteria preparations, such as regulating the micro-ecological balance of the intestinal tract of animals, enhancing the body's immune function and anti-stress capabilities; on the other hand, it is rich in organic selenium, which has high selenium utilization rate and toxicity It has the advantages of low pollution and little environmental pollution; at the same time, the preparation is also rich in glutathione and L-lactic acid, which can effectively improve the efficiency of livestock and poultry breeding. technical background [0002] Adding antibiotics to livestock and poultry feed can improve breeding efficiency. However, the excessive use of antibiotics will bring problems such as drug residues and bacteria...

Claims

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Application Information

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IPC IPC(8): A23K1/00C12N1/00C12N1/16C12N1/18C12N1/20C12R1/865C12R1/72C12R1/125C12R1/10C12R1/245
Inventor 朱建航张帆邹晓阳谢莉胡波平
Owner 江西昌丰由由生物科技有限公司
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