A kind of Aspergillus tubingensis and its application in the preparation of turmeric saponin

Abstract

The invention discloses an Aspergillus tubingensis HG‑57 and its application in preparing turmeric saponin, the preservation number of which is CCTCC M2016389. An appropriate amount of Aspergillus tubingensis mycelium was inoculated on a PDA solid medium for activation, and after spore production, water was added to collect the spore suspension. The spore suspension is inserted into the seed culture medium to amplify to obtain the seed solution. The spore suspension or the amplified seed solution is added to the turmeric water extract, the turmeric alcohol extraction solution or the turmeric water mixture, and the fermented liquid is obtained after aerobic fermentation for 1 to 7 days; the saponin product is obtained by separating and extracting from the fermented liquid. Compared with the saponin content obtained by directly adding acid hydrolysis in the above system, the content is respectively increased by 1.2-1.4 times, 1.1-1.4 times, and 1.4-1.8 times. The strain can effectively convert turmeric saponin into saponin, completely replace the acid hydrolysis conversion process in the traditional process, and is very easy for engineering amplification, and has broad industrial application prospects.

Description

technical field [0001] The invention belongs to the field of microbiology and biotechnology, and more specifically relates to an Aspergillus tubingensis and its application in preparing turmeric saponin. Background technique [0002] Diosgenin, also known as saponin, is an important raw material for the production of steroid hormone drugs. More than 60% of the global steroid hormone drugs are produced synthetically or semi-synthetically with diosgenin as raw material. The traditional method of producing diosgenin is acid hydrolysis with strong acid, but this method has high pollution, high energy consumption and affects the water quality safety of the South-to-North Water Transfer Project. At the same time, in the process of acid hydrolysis, there are incomplete conversion of saponin and by-products are easily produced, which will affect the quality of saponin products. The current research generally believes that enzymatic hydrolysis and microbial transformation have good...

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Problems solved by technology

[0003] At present, some researchers have found that Fusarium exocytoma WX12 (CN 102703329 A) has the function of transforming and producing diosgenin, but it needs to add a series of inorganic salt components during the transformation process from the seed liquid to culture the bacteria and put it into the fermentation liquid. , the time spent is too long, the yield of diosgenin is low, and a considerable amount of water-soluble saponin is lost in the process of preparing turmeric saponin

Some researchers also found that Aspergillus oryzae (CN 101012474 B) can convert saponin into diosgenin, but there are many intermediate products and low conversion rate, so it is difficult to realize industrial application

Another researcher found that Aspergillus awamori (CN104232724 A) can convert turmeric saponin to produce diosgenin. Due to the binding effect of starch and cellulose in turmeric dry powder on saponin, the strain disclosed in the patent failed to convert part of the bound saponin, resulting in the production of diosgenin. low rate

There is research on the use of Lactobacillus acidophilus (CN 103497987 B) to transform turmeric saponin to produce diosgenin, which adopts the method of separating starch, cellulose and diosgenin and then intercepting diosgenin by nanofiltration. The comparison of the yield of saponin obtained by direct acid hydrolysis cannot correctly evaluate the transformation efficiency of the strain in the case of large differences in the quality of turmeric from different origins

Existing patents report that in the process of microbial transformation of turmeric saponin to produce saponin, the process is complicated, the form of converted saponin is single, the conversion efficiency is low, and the yield of saponin is low, so it is difficult to realize industrial application

[0004] Patent CN 103242418 A discloses a clean production process of turmeric saponin, using saccharification, membrane separation and ultrasonic extraction technologies to obtain saponin membrane concentrate and organic solvent saponin extraction concentrate, which can be more comprehensively used in industrialization level to obtain saponin, but in the process of converting saponin into saponin, acid hydrolysis is still used to catalyze the hydrolysis of saponin into saponin, which fails to achieve real green production

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