A high-salt-resistant colorimetric sensor based on zinc-based functional nucleic acid and its application

A technology of colorimetric sensors and functional nucleic acids, applied in instruments, scientific instruments, genetic engineering, etc., can solve the problems of poor selectivity, high price, and low sensitivity, and achieve the effects of low cost, high sensitivity, high specificity, and sensitivity
CN107966423BActive Publication Date: 2020-10-02CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2020-10-02

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Abstract

The invention discloses a high salt tolerant colorimetric sensor based on functional nucleic acid of zinc and application thereof. The high salt tolerant colorimetric sensor includes a molecular recognition element, a signal amplification element, and a signal conversion element. The molecular recognition element consists of zinc ion deoxyribozyme, which is composed of a substrate chain and an enzyme chain. The signal amplification element includes an isothermal amplification system, and the isothermal amplification system also includes an amplification template. The signal conversion elementincludes thioflavin. The molecular recognition element recognizes zinc ions and produces an amplification product, the amplification product forms a G-quadruplex structure under the action of thioflavin, and the fluorescence intensity is detected to calculate the zinc ion concentration. The sensor provided by the invention is based on zinc ion deoxyribozyme, isothermal index amplification reactionand G-quadruplex liquid phase sensing technology, can be used for field detection of zinc ions in the environment, and has the advantages of simple and rapid operation, low cost, high sensitivity andgood selectivity.
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Description

technical field

[0001] The invention belongs to the technical field of ion detection, and in particular relates to a high-salt-resistant colorimetric sensor based on zinc-based functional nucleic acid and an application thereof. Background technique

[0002] Heavy metal zinc (Zinc, Zn) is a widely distributed metal element in nature, mainly exists in the state of zinc sulfide and zinc oxide, and can also coexist with minerals of many elements such as lead, copper, and zinc. Zinc pollution refers to the environmental pollution caused by zinc and its compounds. The main sources of pollution are zinc mining, smelting and processing, machinery manufacturing, galvanizing, instrumentation, organic synthesis, and papermaking industries. Automobile tire wear and dust and smoke produced by coal combustion all contain zinc and its compounds. Zinc in industrial wastewater often exists in the form of zinc hydroxyl complexes.

[0003] Zinc is the most abundant trace element in the huma...

Claims

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