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Application of lentinula edodes dietary fiber extract in preparation of preparations for treating and/or preventing liver injury-related diseases caused by high fat diet

A dietary fiber and high-fat diet technology is used in health care products. In the field of pharmaceuticals, the dietary fiber extract of shiitake mushrooms can solve the problems of increased fat mass and decreased bacterial diversity, so as to inhibit the expression of inflammatory factors, alleviate the deterioration of inflammation, The effect of large economic and social values

Inactive Publication Date: 2018-08-10
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing studies have confirmed: (1) Intestinal flora is related to obesity. Obese people often have intestinal flora structure disorder and bacterial diversity decrease; obese mice have fewer Bacteroidetes and more Firmicutes; Transplanting gut microbes from obese mice into germ-free mice increases fat mass

Method used

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  • Application of lentinula edodes dietary fiber extract in preparation of preparations for treating and/or preventing liver injury-related diseases caused by high fat diet
  • Application of lentinula edodes dietary fiber extract in preparation of preparations for treating and/or preventing liver injury-related diseases caused by high fat diet
  • Application of lentinula edodes dietary fiber extract in preparation of preparations for treating and/or preventing liver injury-related diseases caused by high fat diet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation method of mushroom dietary fiber extract, the steps are as follows:

[0044](1) Dried shiitake mushrooms were crushed and weighed. According to the ratio of material to liquid (mass volume ratio) of 1:10, 95% ethanol was used to boil and reflux to extract for 2 hours, and suction filtered to obtain the filter residue;

[0045] (2) Add water to the filter residue, the ratio of material to liquid (mass volume ratio) is 1:10, boil and reflux for extraction for 2 hours;

[0046] (3) When the temperature drops to about 60°C, adjust the pH value to 6-7, add papain for enzymolysis, the enzyme amount is 0.2% (mass percentage) of the sample weight, and enzymolyze in a water bath at 60°C for 1 hour. stop stirring;

[0047] (4) After the enzymolysis is completed, add 95% ethanol in an amount 4 times the volume of the extract after enzymolysis to precipitate overnight, and filter with suction to obtain a filter residue;

[0048] (5) The filter residue is eluted wi...

Embodiment 2

[0052] Example 2: Improvement effect of mushroom dietary fiber extract on liver injury in obese mice induced by high-fat diet

[0053] 1. Reagents:

[0054] Total cholesterol (T-CHO) test kit: Nanjing Jiancheng Institute of Bioengineering.

[0055] Triglyceride (TG) test kit: Nanjing Jiancheng Institute of Bioengineering.

[0056] Low-density lipoprotein cholesterol (LDL-C) test kit: Nanjing Jiancheng Bioengineering Institute.

[0057] High-density lipoprotein cholesterol (HDL-C) test kit: Nanjing Jiancheng Bioengineering Institute.

[0058] Alanine Aminotransferase (ALT) Test Kit, Aspartate Aminotransferase (AST) Test Kit, Total Protein (TP) Test, Albumin (ALB) Test and Total Bilirubin (T-Bil) Test Kit All were purchased from Nanjing Jiancheng Institute of Bioengineering.

[0059] Thirty female C57 mice, SPF grade, weighing 12-14 g, and 28-30 days old, were purchased from Guangdong Medical Experimental Animal Center with license number SCXK (Guangdong) 2013-0002.

[0060...

Embodiment 3

[0097] Example 3: Improvement effect of mushroom dietary fiber extract on diseases related to intestinal flora imbalance in obese mice induced by high-fat diet

[0098] 1. Experimental method

[0099] The feces of the mice at the end of the animal experiment in Example 2 were collected, quickly frozen with liquid nitrogen, and stored in a -80°C refrigerator. DNA was extracted from stool samples, and the quality of DNA extraction was detected by 0.8% agarose gel electrophoresis, and DNA was quantified by UV spectrophotometer. Forward primer 5'-ACTCCTACGGGAGGCAGCA-3' and reverse primer 5'-GGACTACHVGGGTWTCTAAT-3' were used to amplify the V3-V4 region gene of mouse 16S rRNA. The PCR amplification products were detected by 2% agarose gel electrophoresis, and the target fragments were recovered by gel cutting. Referring to the preliminary quantitative results of electrophoresis, the recovered products of PCR amplification were quantified by fluorescence. The fluorescent reagent wa...

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Abstract

The invention relates to use of a lentinula edodes dietary fiber extract in the pharmaceutical field, in particular to application of the lentinula edodes dietary fiber extract in preparation of preparations for treating and / or preventing liver injury-related diseases caused by high fat diet. The lentinula edodes dietary fiber extract not only can obviously contribute to the recovery of the dominant flora structure in the intestinal tract and is beneficial to the diversification of the flora, but also shows obvious treatment and / or prevention effect on the liver injury-related diseases causedby high fat diet; the lentinula edodes dietary fiber extract can effectively inhibit the expression of inflammatory factors, effectively alleviates the deterioration of inflammation, improves variousphysiological and pathological indicators related to immunity, and can be used in various fields such as medicines and health care products, thus having great economic and social values.

Description

technical field [0001] The present invention relates to the use of mushroom dietary fiber extract in the fields of pharmacy and health care products, in particular to the use of mushroom dietary fiber extract in preparing preparations for treating and / or preventing diseases related to liver damage caused by high-fat diet. Background technique [0002] Obesity refers to the excessive accumulation of fat in the human body, which obviously exceeds the average amount of normal people, and there are abnormal changes in body metabolism, physiology and biochemistry, which is called obesity. As of 2014, 600 million people in the world are obese. It is estimated that by 2025, the number of obese people in the world will reach 1.4 billion. Obesity has become one of the public health problems that attract worldwide attention. Studies have found that the occurrence and development of common diseases in wealthy societies are mostly related to obesity, such as diabetes, hypertension, coro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715C08B37/00A23L33/21A61P1/16A61K36/07
CPCA23L33/21A23V2002/00A61K31/715A61K36/07A61P1/16C08B37/0003A23V2200/32A23V2250/208
Inventor 陈地灵唐晓萃杨俭帅欧赖国校谢意珍吴清平杨柏华
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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