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Biocontrol Phoma macrostoma strain P2 and application thereof

A technology for Pythium stipitis and Pythium melon and fruit is applied in the field of Pythium giganta to achieve the effects of inhibiting the growth of pathogenic bacteria, strong resistance to high temperature and avoiding adverse effects

Active Publication Date: 2019-05-03
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Graupner once reported in 2003 that Phomamacrostoma (Phomamacrostoma) can produce novel cyclic tetramic acid (novel cyclic tetramic acid) as a herbicide to control weeds on the lawn, such as thistle, dandelion, clematis and other broad-leaved weeds. Grass, but there is no report on the biological control of plant pathogens by using Phomoma coelococcus at home and abroad

Method used

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  • Biocontrol Phoma macrostoma strain P2 and application thereof
  • Biocontrol Phoma macrostoma strain P2 and application thereof
  • Biocontrol Phoma macrostoma strain P2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Isolation, Identification and Biological Characteristic Analysis of P2 Strain of Phomoma coelococcus

[0041] 1. Isolation of strains and analysis of their biological characteristics

[0042] The applicant selected the sick and disabled body with typical symptoms from the rapeseed black shank samples collected in Yunxi County, Hubei Province, and cut the tissue block at the junction of the disease and health, with a size of about 0.5cm×0.5cm; with 75% ethanol , 5% sodium hypochlorite for 30s and 5min, respectively, and then washed continuously with sterile water for 3 times, then transferred to the center of the PDA medium plate containing 25% lactic acid, incubated at 22°C for 4 days, and finally grew out from the edge of the diseased tissue fungi were purified. In addition, four fungi (Phoma sp.) similar to the pathogenic bacteria of rapeseed blackleg were isolated from rapeseed blackleg samples in our laboratory, named P1, P2, P3, and P4. Through pathogenicity analy...

Embodiment 2

[0052] Purification and Fermentation of Phomatopoints macrocoelom P2

[0053] (1) Strain activation: Pick a piece of P2 strain slant stored in a 4°C refrigerator with a sterile inoculation needle, inoculate it on PDA medium, and culture it in a 22°C incubator for 3 days.

[0054] (2) Strain cultivation: the P2 strain cultured for 3 days in step (1) was transferred to fresh PDA medium, and cultured in a 22° C. incubator for 7 days.

[0055] (3) Fermentation: use a sterile puncher to punch several bacterial cakes at the edge of the colony of the P2 strain in step (2), inoculate 5 pieces of mycelium in each 100mL of PDB culture solution, shake and culture at 150rpm and 22°C for 3d and 6d respectively , 9d and 15d, after centrifugation at 6000rpm, the fermented product filtrates of different days were collected respectively for the following examples.

[0056] (4) Fermentation filtrate of Phomopsis macrocoelom P2: place the above filtrate of fermentation product of Phomopsis macr...

Embodiment 3

[0059] Preparation of Filtrate from Fermentation of Phomoma coelococcus P2 and Determination of Antifungal Substances

[0060] 1. The temporal dynamics of antifungal substances produced by the ferment filtrate of Phomoma coelom P2

[0061] The fermented product filtrates were collected on the 3d, 6d, 9d, 12d and 15d of the shaking culture of the P2 strain, and the pH value was measured, and the dry weight of the obtained mycelia was weighed after drying (Table 1). After the fermentation filtrate is filtered by a bacteria filter with a pore size of 0.22 μm, in the ratio of 10% (v / v) (in the present invention, this percentage refers to the P2 fermentation product filtrate: culture medium is 1:9, volume ratio ) was added to the PDA medium and mixed evenly, and the virus-infected plate was obtained by inverting the plate, and 10% (v / v) PDB solution was added as a negative control. Sclerotinia sclerotiorum (Sclerotinia sclerotiorum), Botrytis cinerea (Botrytis cinerea), Rhizoctoni...

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Abstract

The invention belongs to the technical field of agricultural microbiology, and specifically discloses a Phoma macrostoma strain and application thereof. The preservation number of the Phoma macrostomastrain isolated by the inventor is CCTCC NO: M 2018726. Phoma macrostoma or filtrate of fermentation products thereof can effectively inhibit mycelium growth, conidiospore and sclerotia germination of important pathogenic bacteria on rape (including sclerotinia sclerotiorum, botrytis cinerea, rhizoctonia solani and leptosphaeria biglobosa); moreover, the Phoma macrostoma also has anti-fungus effects of relatively broad spectrum, thereby capable of inhibiting mycelium growth of pathogenic fungi on most other plants. In addition, filtrate of a fermentation product of a Phoma macrostoma strain P2 has no toxic effect on rape seeds, so that, rape seeds subjected to soaking treatment with the filtrate of the fermentation product can normally germinate; and thus, the filtrate of the fermentationproduct of the Phoma macrostoma strain P2 can be used for processing rape seeds, thereby preventing and treating soil-borne diseases. Moreover, the Phoma macrostoma strain P2 has very strong resistance to high temperature, acid, alkali and ultraviolet radiation. Thus, the Phoma macrostoma strain P2 has potential for development and utilization.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbiology, and in particular relates to Phomamacrostoma and its application. Background technique [0002] Phoma fungus can cause important diseases of plants. It can harm hundreds of plants such as leguminous, solanaceous, cruciferous and magnolia, causing leaf spot, stem rot and branch dieback of plants. For example, Phoma destructiva is a pathogen that causes tomato leaf and stem blight (Boerema et al, 2004), and Phoma lingam is an important pathogen that causes cruciferous crop stem canker (there are two types of Leptosphaeria maculans / Leptosphaeriabiglobosa ), can cause a 20% to 60% reduction in rapeseed yield. P.andigena and P.tracheiphila in the Phoma genus are listed as local quarantine pathogens in Europe and other regions, P.foveata in the southern United States and P.macdonaldii in Australia and other regions, and have important quarantine properties. In my country, P.exigua, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01C1/06C12R1/645
Inventor 李国庆程均钰罗韬杨龙吴明德张静
Owner HUAZHONG AGRI UNIV
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