Production process of phloretin on basis of fermentation of saccharomyces cerevisiae and saccharomyces cerevisiae

A technology of Saccharomyces cerevisiae and phloretin, applied in the direction of microorganism-based methods, fermentation, microorganisms, etc., can solve the problems of increasing the cost of phloretin and unfavorable industrial production, and achieve small market price fluctuations, low prices, and easy availability of raw materials Effect

Active Publication Date: 2019-08-13
嘉兴欣贝莱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this technology requires the use of expensive phloretin and hydrolase as raw materials, whi

Method used

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  • Production process of phloretin on basis of fermentation of saccharomyces cerevisiae and saccharomyces cerevisiae

Examples

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Embodiment 1

[0032] This example provides a process for activating Saccharomyces cerevisiae by CM-l-u-t medium:

[0033] Thaw the frozen Saccharomyces cerevisiae with the biological deposit number CCTCC NO M2018718 and add it to the defective CM-lut medium at a rate of 1% for cultivation. The medium consists of 6.7 g of amino-free yeast nitrogen source and adenine. 50mg, 100mg histidine, 0.83g dropout powder, dissolved in ultrapure water, adjust the pH to 5.6, dilute to 950mL, autoclave, then cool to room temperature, add 50mL of an autoclaved glucose solution with a concentration of 40% , Mix well and set aside; in this experiment, 150mL of defective CM-lut medium was added to a 250mL Erlenmeyer flask that was autoclaved, and 1.5mL of Saccharomyces cerevisiae was added to activate the strain. Cultivate until the OD600 of the bacterial solution (that is, the absorbance value of the bacterial solution measured at a wavelength of 600nm) reaches 4-6, and the culture conditions: temperature 30°C,...

Embodiment 2

[0035] This example provides the YPAD medium expansion culture process:

[0036] Use a pipette to precisely pipette 10 mL of the bacterial solution in Example 1, and add it to a 2L conical flask containing 1000 mL of YPAD medium after being autoclaved at a rate of one percent. The composition is: 10 g of yeast extract, 50 mL of adenine and 20 g of peptone are dissolved in 950 ml of ultrapure water, autoclaved, and after cooling to room temperature, 50 mL of an autoclaved glucose solution with a concentration of 40% is added.

[0037] Yeast was cultured in YPAD medium, and cultivated until the OD600 of the bacterial solution reached 4.5-9. The culture conditions: temperature 30℃, rotation speed 220 r / min.

Embodiment 3

[0039] This embodiment provides a fermentation process in a fermentor:

[0040] First, in the fermentation process, take the bacterial liquid in the above example 2 for fermentation in a fermentor. The complete steps are as follows: prepare a fermentation medium. The 3000ml fermentation medium consists of 66g glucose, 45g ammonium sulfate, and potassium dihydrogen phosphate. 24g, 18.6g magnesium sulfate heptahydrate, 6g dropout powder, 30mL trace metal solution, 3mL calcium chloride dihydrate with a concentration of 2g / L, and 7.5g histidine dissolved in 2877mL ultrapure water, autoclaved and cooled to After room temperature, add 75mL of adenine aqueous solution with a concentration of 10% filtered and sterilized and 48mL of filtered and sterilized Vitamin solution (vitamin solution), mix well, and set aside. The vitamin solution is composed of: weigh 0.05 g of biotin, 1 g calcium pantothenate, 1 g niacin, 25 g inositol, 1 g thiamine, 1 g pyridoxal and 0.2 g p-aminobenzoic acid, d...

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Abstract

The invention provides a saccharomyces cerevisiae. The biological preservation number of the saccharomyces cerevisiae is CCTCC NO M2018718, and a production process of phloretin is obtained through research in combination with the saccharomyces cerevisiae and mainly comprises the processes of activation of a saccharomyces cerevisiae strain in a culture medium, expansion culture, fermentation culture in a fermentation tank, inoculation, material replenishing and fermentation production. The provided production process of the phloretin has the advantages that the cost is low, requirements for equipment are simple, produced reagents are free of toxic substances and harmful substances, and the production process is in line with an environment-friendly production concept; moreover, the yield ofthe phloretin is considerable.

Description

Technical field [0001] The invention belongs to the technical field of phloretin synthesis, and particularly relates to a Saccharomyces cerevisiae and a process for synthesizing phloretin by using Saccharomyces cerevisiae. Background technique [0002] Yeast is a commonly used eukaryotic receptor cell in gene cloning experiments, and its culture method is simple. There are also many types of yeast cloning vectors. Yeast also has plasmids. This 2pm long plasmid is called 2um plasmid, about 6300bp. This plasmid exists outside the chromosomes in the nucleus for at least a period of time. The 2pm plasmid and the plasmid in E. coli can be used to construct a shuttle plasmid that can shuttle between bacteria and yeast cells. Yeast cloning vectors are all constructed on this basis. of. Phloretin is a natural skin whitening agent recently researched and developed at home and abroad. Phloretin can inhibit the excessive secretion of sebaceous glands and is used to treat exuberant acne; ...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12P7/26C12R1/865
CPCC12P7/26C12R2001/865C12N1/185
Inventor 陈贤情盛夏鑫王筱王文江会锋
Owner 嘉兴欣贝莱生物科技有限公司
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