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Construction method of electrochemical biosensor based on biomass carbon-nanogold, and analysis application

A technology of biosensor and biochar, which is applied in the field of electrochemical biosensor construction, can solve the problems of destroying the ability to transport oxygen, complicate instruments and equipment, and endanger human health, so as to facilitate charge transmission, improve sensitivity, and low cost Effect

Pending Publication Date: 2019-09-03
HAINAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Trichloroacetic acid (TCA), as an organic halide, can be used in herbicides, freckle-removing beauty agents, and drinking water fungicides, etc., entering the human body can destroy the protein structure of the human body and endanger human health; sodium nitrite (NaNO 2 ) is often used as a food additive, but when this substance enters the organism, it can produce carcinogenic amine nitrite, which can oxidize low iron hemoglobin in the blood to methemoglobin, destroy the ability of the blood in the organism to transport oxygen, and endanger human health; Potassium bromate ( KBrO 3 ) is widely used in the food industry as a quality-improving additive for flour and canned fish products. This substance will inhibit the activity of proteolytic enzymes, thereby producing carcinogens and endangering human health; therefore, an efficient, simple and sensitive method is established. Quantitative analysis method to detect TCA, NaNO 2 and KBrO 3 The content of
[0005] At present, common detection methods have defects such as poor sensitivity, narrow linear range, and the need for more complex and expensive instruments and equipment, so it is very important to develop a simple, cheap, efficient and sensitive quantitative analysis method

Method used

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  • Construction method of electrochemical biosensor based on biomass carbon-nanogold, and analysis application
  • Construction method of electrochemical biosensor based on biomass carbon-nanogold, and analysis application
  • Construction method of electrochemical biosensor based on biomass carbon-nanogold, and analysis application

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Effect test

Embodiment 1

[0035] A construction method and analytical application of an electrochemical biosensor based on biomass carbon-nano-gold, including CILE used as a base electrode in a three-electrode system. The surface of CILE is modified with a composite film, and the composite film is arranged sequentially from the inside to the outside The composition of BC, Au, Hb and Nafion, the steps of analysis application are as follows.

[0036] When the above bioelectrochemical sensor was placed in PBS containing TCA at a pH of 3.0, the cyclic voltammetry curve was recorded, and a linear regression equation was constructed according to the relationship between the concentration of TCA and the reduction peak current. see figure 1 , which are the cyclic voltammetry curves when the above-mentioned electrochemical biosensor detects 10.0, 20.0, 300.0, 330.0 mmol / L TCA solution respectively It can be clearly seen that the reduction peak current increases with the increase of TCA concentration.

[0037]...

Embodiment 2

[0041] A method for constructing an electrochemical biosensor based on biomass charcoal-nano gold comprises the following steps.

[0042] S1, get 1.6 g graphite powder and 0.8 g N-hexylpyridine hexafluorophosphate (HPPF 6 ) was ground in a mortar for 2.5 hours to obtain a carbon paste, and then the carbon paste was filled into a glass electrode tube with an inner diameter of 4 mm for compaction, and a copper wire was inserted as a wire to obtain CILE. Figure 4 Curve a shows the cyclic voltammetry of CILE in PBS at pH 3.0.

[0043] S2. Nafion modification: the surface of CILE obtained in step S1 is modified with 0.5% wt Nafion ethanol solution to obtain Nafion / CILE. Figure 4 Curve b shows the cyclic voltammetry curve of Nafion / CILE in PBS at pH 3.0.

[0044] S3. Modification of Hb: modify the surface of CILE obtained in step S1 with 8.0 μL of 15.0 mg / mL Hb solution to obtain Hb / CILE; apply 0.5% wt Nafion ethanol solution on the surface of Hb / CILE, and obtain Nafion after st...

Embodiment 3

[0049] An application of the bioelectrochemical sensor of embodiment 1 in detecting TCA comprises the following steps: using Nafion / Hb / Au-BC / CILE prepared in embodiment 2 S6 as a working electrode, and an Ag / AgCl electrode as a reference electrode, The platinum electrode was used as the counter electrode, and a three-electrode system was established, and PBS with a pH of 3.0 was used as the supporting electrolyte, and the cyclic voltammetry curve was recorded; a detection linear regression equation was constructed according to the relationship between the concentration of TCA and the reduction peak current. refer to Figure 5 , the detection linear regression diagram of the relationship between TCA concentration and reduction peak current change. From the figure, it can be seen that the detection linear regression equation is: Ip (μA)=6.32C(mmoL / L)+61.9, the correlation coefficient is g=0.990, and the TCA detection linearity The range is 10.0~330.0 mmol / L, and the detection li...

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Abstract

The invention discloses a construction method of an electrochemical biosensor based on biomass carbon-nanogold. The construction method is further applied to the quantitative analysis of trichloroacetic acid, sodium nitrite and potassium bromate. An ion liquid carbon paste electrode modified with biomass carbon (BC), the nanogold (Au), hemoglobin (Hb) and Nafion composite membrane on the surface is used as a working electrode. The construction method comprises the preparation of the biomass carbon material, the electro-deposition of Au, Hb and Nafion membrane assembling and like steps. The constructed electrochemistry biosensor can be applied to the quantitative detection of the trichloroacetic acid, sodium nitrite and potassium bromate, and has the advantages of being strong in interference resistance, wide in detection range and low in detection limit.

Description

technical field [0001] The invention relates to a construction method of an electrochemical biosensor based on biomass charcoal-nano gold, and is used in the fields of preparation of chemically modified electrodes and quantitative analysis of trichloroacetic acid, sodium nitrite and potassium bromate. Background technique [0002] In recent years, biochar has attracted widespread attention due to its advantages of simple preparation method, large specific surface area, porous structure and excellent electrical conductivity. Biochar is a carbon-rich porous particle obtained by high-temperature carbonization of carbon-containing biomass in an oxygen-free environment. There are many types of carbon sources for biochar materials, including crop straw, waste from food processing, and domestic waste produced by humans. As a renewable material, biochar has broad application prospects in the fields of electrochemistry and new energy, and it can be used in the construction of superc...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N27/36G01N27/48
CPCG01N27/308G01N27/3277G01N27/3278G01N27/36G01N27/48
Inventor 孙伟罗贵铃殷春晓邓英邹如意牛燕燕刘娟李冰航陈鹏颖
Owner HAINAN NORMAL UNIVERSITY
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