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A rare earth fluoride hybrid nanoflower and its preparation method

A rare earth fluoride and nanoflower technology, applied in the field of nanomaterials, can solve the problems of difficulty in controlling the morphological characteristics of nanoflowers, nanoflower structure, complex synthesis process of nanoflowers, unfavorable protein activity, etc., and achieves good immobilization effect, mild conditions, Simple preparation method

Active Publication Date: 2021-10-29
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although various forms of nanoflowers have been developed, the synthesis process of these nanoflowers is complicated and requires relatively harsh conditions, such as the use of a large amount of toxic organic solvents, and high temperature and high pressure reaction conditions, and even under the above harsh conditions, it is still difficult to control the morphological characteristics of nanoflowers and the structure of nanoflowers
[0004] At present, rare earth element hybrid nanoflowers have been reported, and their preparation methods mainly include chemical vapor deposition (CVD), sol-gel method, microemulsion method, non-hydrolysis route and hydrothermal method, etc. The reaction conditions are harsh, the energy consumption is high, and the environmental requirements are high, which is not conducive to maintaining the activity of the protein

Method used

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  • A rare earth fluoride hybrid nanoflower and its preparation method
  • A rare earth fluoride hybrid nanoflower and its preparation method
  • A rare earth fluoride hybrid nanoflower and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Trypsin-Na 5 Yb 9 f 32 preparation of

[0036]Add 1.0mg trypsin to 0.685mL water respectively, then add 0.1mL (0.5M) ytterbium nitrate solution, and stir for 1 hour to obtain a colorless, transparent and uniform liquid; then slowly add 0.3mL (0.5M) Sodium fluoride solution, get white floc, stir for 10min, place in 25°C incubator for 2 days, get white precipitate; centrifuge the precipitate at 10000rpm, discard the supernatant, leave the solid; use absolute ethanol Disperse the solid, purify and centrifuge three times, and dry in a constant temperature drying oven at 25°C to obtain the hybrid nanoflower trypsin-Na 5 Yb 9 f 32 .

[0037] The prepared hybrid nanoflower trypsin-Na 5 Yb 9 f 32 Perform EDX, XPS, SEM, TEM, FT-IR, TGA, electron diffraction and energy dispersive X-ray analysis.

[0038] The result is as figure 1 and figure 2 shown, where figure 1 a is an XRD pattern, in which the diffraction peaks and trypsin-Na 5 Yb 9 f 32 correspondi...

Embodiment 2

[0039] Example 2 Photoprotein-Na 5 Yb 9 f 32 :Tb 3+ preparation of

[0040] Add 1.0mg trypsin, 4.0mg lysozyme, 1.0mg recombinant collagen Ftsz, 1.0mg bromelain, 4.0mg bovine serum albumin and no protein in 0.675mL water, then add 0.1mL (0.5M) ytterbium nitrate solution, 10 μL (0.2M) terbium nitrate solution was mixed evenly, and after stirring for 1 hour, a colorless, transparent and uniform liquid was obtained; then 0.3mL (0.5M) sodium fluoride solution was slowly added dropwise to the solution to obtain a white flocculent The product was stirred for 10 minutes, and placed in a thermostat at 25°C for 2 days to obtain a white precipitate; the product was centrifuged at 10,000 rpm, the supernatant was discarded, and the solid was left; the solid was dispersed with absolute ethanol, and then purified and centrifuged for 3 times. Dry in a constant temperature drying oven at 25°C to obtain photoproteins of different protein types-Na 5 Yb 9 f 32 Hybrid nanoflowers.

[0041]...

Embodiment 3

[0043] Example 3 Trypsin-Na 5 Yb 9 f 32 :Tb 3+ activity assay

[0044] Using casein as a substrate, a casein solution (10 g / L) was prepared in 50 mM PBS buffer (pH 7.4). Trypsin-Na 5 Yb 9 f 32 :Tb 3+ Add to 1mL of 10g / L casein, incubate at 30°C for 30min, and centrifuge at 9000rpm for 5min to collect the supernatant; add trichloroacetic acid (TCA) solution (2mL, 0.4M) to the supernatant, and Let stand for 5 minutes to terminate the enzymatic reaction, and collect the supernatant by centrifugation; mix 5 mL of sodium carbonate solution (0.4 M) and 1 mL of Folin’s phenol reagent with 1 mL of the supernatant, and incubate the mixture at 40°C for 20 minutes to complete color development Reaction; the optical density at 763 nm was monitored to assess the hydrolytic properties of casein. The activity of free trypsin was assessed by the same procedure using the corresponding amount of trypsin in the hybrid nanoflowers.

[0045] Trypsin-Na under different amounts of urea or ...

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Abstract

The invention belongs to the technical field of nanometer materials, and in particular relates to a rare earth fluoride hybrid nanoflower and a preparation method thereof. The present invention uses protein as a mineralization template and rare earth fluoride as an inorganic component. Compared with the prior art, the synthesis of protein-rare earth fluoride hybrid nanoflowers can be realized under low temperature conditions. The method has mild reaction conditions and is green. Environmental protection, low energy consumption; the prepared hybrid nanoflowers have a high specific surface area, and on the basis of ensuring the stability and activity of the protein to the greatest extent, the unique luminescence characteristics of lanthanide metals are added; the immobilization effect of this method on the protein It is good and will not affect the luminescent properties of the original lanthanide metals; the prepared nanoflowers are doped with different kinds of lanthanide ions, and have great application potential in biosensing, biocatalysis, and medical diagnosis and treatment.

Description

technical field [0001] The invention belongs to the technical field of nanometer materials, and in particular relates to a rare earth fluoride hybrid nanoflower and a preparation method thereof. Background technique [0002] Lanthanide element (lanthanide element) is a collective name for 15 chemical elements with atomic numbers 57-71 in group IIIB of the periodic system of elements. Since lanthanide elements are all metals, they are also called lanthanide metals. Lanthanide elements are represented by the symbol Ln, including lanthanum, cerium, praseodymium, neodymium, promethium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, and lutetium, which are also members of rare earth elements. Due to the good luminescent properties of rare earth metal elements, they can be widely used in luminescent sensing for biological and chemical detection. [0003] Nanoflower (Nanoflower) belongs to a kind of nanomaterial, which has a higher surfac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/85C07K17/14C12N11/14C07K17/02C12N11/02
CPCC07K17/02C07K17/14C09K11/7773C12N9/2462C12N9/48C12N9/6427C12N11/02C12N11/14C12Y302/01017C12Y304/21004C12Y304/22031
Inventor 肖建喜魏文宇
Owner LANZHOU UNIVERSITY
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