Bone implant material based on nano-enzyme drug modification and preparation method and application thereof

Abstract

The invention discloses a bone implant material based on nano-enzyme modification and a preparation method and application thereof. The bone implant material based on nano-enzyme modification is obtained by modifying a bone implant material with nano-enzyme or a precursor of the nano-enzyme (the nano-enzyme can be prepared in situ), and the bone implant material, as an efficient orthopedic treatment mode, is directly related to the therapeutic effect due to the influence and regulation effect on the metabolic balance of bone tissues. According to the invention, the ROS elimination capability and ALP hydrolytic activity of the nano-enzyme in a specific environment are utilized to enhance the osseointegration capability and osteolysis inhibition effect of the bone implant material; in addition, bacterial adhesion or growth is inhibited by using the ROS generation capability and the multi-hydrolytic activity of the nano-enzyme, and bacterial biofilms are destroyed to enhance an anti-infection effect of combined antibiotics at the same time. By means of an efficient complementary design, the invention has therapeutic characteristics in multiple aspects, and bone metabolism imbalance diseases related to the bone implant material can be treated, relieved and / or prevented.

Description

technical field [0001] The invention belongs to the interdisciplinary field of biomedicine and material engineering, and in particular relates to a nanozyme-based drug-modified bone implant material and its preparation method and application. Background technique [0002] Bone tissue is one of the most important organs of human beings. With the improvement of people's living standards and the increase of aging, more and more orthopedic diseases have not been solved well. Among them, the treatment method of acting or (and) enhancing the function of bone tissue by implanting materials has become an efficient treatment method, but as the population of implants becomes younger, the amount of exercise of patients increases, and the proportion of osteoporosis population increases, at the same time There is also a great risk of implant infection, resulting in unsatisfactory postoperative healing or revision problems still exist. [0003] Bone tissue maintains metabolic balance (i...

AI Technical Summary

Problems solved by technology

[0011] First, most of the existing bone implant materials do not have the effect of actively promoting osseointegration. Since most patients are elderly with insufficient bone mass production, it is very important to actively promote osteogenesis.

[0012] Second, the existing bone implant materials, such as titanium alloy, bone cement, and polyethylene, will produce wear particles after long-term service, and these wear particles will enhance local inflammation and ROS, and eventually cause osteolysis and bring about non-bacterial loose sex

The existing method is to increase the wear resistance of the material and reduce the generation of particles, but there are no or few materials that can reduce inflammatory stimulation and actively eliminate ROS

[0013] Third, the surface of joint replacement and orthopedic implant materials is an environment that is prone to bacterial growth and biofilm formation. In addition, the material implantation site is an environment with insufficient blood supply, which greatly limits drug delivery, and the barrier of dense biofilm hinders Penetration of antibacterial drugs, resulting in the ineffective bactericidal effect of low-concentration drugs, which is also an important reason why bone implant materials are difficult to repair after infection at this stage

Increasing the molecular weight and cross-linking of polyethylene enhanced its mechanical properties and wear resistance without reducing the inflammation-inducing effect of the wear particles themselves

As a reducing agent, vitamin E protects polyethylene through anti-oxidation, but as a consumable molecule, bone implant materials will have the problem of decreased protection ability after implantation for a long time (10-15 years).

[0017] Third, for bone implant material infection, the direct use of antibiotics or the use of antibiotic-loaded bone cement has become the main clinical treatment method at this stage, but the release of drugs is difficult to control, and the side effects are obvious after long-term use

[0019] Difficulties in nanozyme modification of bone implant materials at this stage: (1) It is necessary to prepare nanozymes with enhanced hydrolysis activity and ROS elimination activity, and to explore ways to immobilize and modify nanozymes on the surface of bone implant materials or uniformly disperse them; ( 2) No nanozyme-modified polyethylene and bone cement bone implant materials have been found at this stage, which can prevent non-bacterial loosening caused by osteolysis; (3) There is no nanozyme-modified bone implant at this stage Bone implant materials that use nano-enzymes to hydrolyze biofilms to enhance the antibacterial effect of antibiotics

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