Pediococcus acidilactici DY15 and application thereof, and feed and preparation method thereof

A technology of Pediococcus lactis and feed, applied in the biological field, can solve the problems of inability to accurately determine the maximum addition ratio, affect the digestion and absorption of nutrients, and unfavorable digestion and absorption of animals, so as to achieve a good intestinal microbial system, improve immunity, improve palatability effect

Pending Publication Date: 2022-02-18
SHANDONG BOHI INDUSTRY CO LTD +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitation of the nutrient composition and feeding value evaluation of unconventional feed materials, it is impossible to accurately determine the maximum addition ratio when formulating diets
In addition, because most of the total carbohydrates in palm meal are non-starch polysaccharides such as mannan that are difficult to digest, there are also anti-nutritional factors such as glycinin in soybean hulls that affect the digestion and absorption of nutrients, and the content of polypeptides is low, which is not conducive to Digestion and Absorption in Animals

Method used

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  • Pediococcus acidilactici DY15 and application thereof, and feed and preparation method thereof
  • Pediococcus acidilactici DY15 and application thereof, and feed and preparation method thereof
  • Pediococcus acidilactici DY15 and application thereof, and feed and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0049] In the fifth aspect, the present invention provides a method for preparing a feed, comprising the following steps: mixing a substrate with alkaline protease, mannanase, and the above-mentioned Pediococcus acidilactici DY15 or bacterial agent, and preparing by fermentation feed;

[0050] Such substrates include palm meal and soybean hulls.

[0051] The preparation method provided by the invention is simple and convenient, and the palm meal and soybean hulls are processed in the way of co-fermentation with bacteria and enzymes, which improves the antibacterial ability and organic acid content of the palm meal and soybean hulls, and can prevent the pathogenic bacteria from spreading in the intestinal tract of animals. Colonization, enhance the disease resistance of animals, reduce anti-nutritional factors in raw materials, improve the utilization rate of nutrients in feed and the digestibility of animals, and play a role in improving the feeding value of palm meal and soyb...

Embodiment 1

[0066] The screening of embodiment 1 bacterial strain

[0067] 1 Medium: MRS medium (g / L): peptone 10.0, beef extract 8.0, yeast powder 4.0, glucose 20.0, dipotassium hydrogen phosphate 2.0, triammonium citrate 2.0, sodium acetate 5.0, magnesium sulfate heptahydrate 0.58, four Manganese sulfate water 0.25, Tween 80 1mL, distilled water 1L, sterilized at 115°C for 20 minutes.

[0068] 2 Screening: The strain was isolated from sauerkraut purchased from Auchan Supermarket in Wuxi. The sauerkraut was first placed in MRS medium at 37°C for 24 hours, and the culture solution was diluted 100 and 1000 times with sterile water, and 100 μL was spread on the MRS solid. Culture medium, cultured at 37°C for 24 hours, a strain of lactic acid bacteria obtained by streaking (such as figure 1 shown). Its microbiological characteristics are typical positive Gram staining, medium-sized bacteria, milky white colonies on the culture medium, smooth and moist surface, raised, and a peculiar sour s...

Embodiment 2

[0070] Example 2 Identification of Physiological and Biochemical Characteristics of Pediococcus lactis DY15

[0071] (1) Litmus milk experiment: Milk mainly contains lactose and casein, in which litmus is added as indicator and redox indicator, litmus is lavender when neutral, pink when acidic, blue when alkaline When the color is restored, the milk fades from top to bottom and returns to white. Lactic acid bacteria ferment lactose to produce acid, litmus turns red, and when the acidity is high, it can make milk coagulate. Observe the results of the litmus milk experiment after inoculation and cultivation, and observe the acid production and coagulation reaction of litmus milk after culturing at 37°C for one day.

[0072] (2) Starch hydrolysis experiment: Inoculate the strains in the basal medium containing soluble starch, incubate at 37°C for 24 hours, take a little culture solution in the colorimetric tray, and take the uninoculated culture solution as a control, add Lugol...

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Abstract

The invention provides pediococcus acidilactici DY15 and application thereof, and feed and a preparation method thereof, and relates to the technical field of biology. It is found through research by the inventor that the pediococcus acidilactici DY15 provided by the invention has bacteriostatic activity and has a good inhibition effect on growth of escherichia coli, salmonella and staphylococcus aureus, and a fermentation product of the pediococcus acidilactici DY15 comprises various flavor substances and can be applied to the field of feed to increase the nutritional value of feed, improve the palatability, improve the immunity of livestock, and establish a good intestinal microorganism system. According to the preparation method of the feed provided by the invention, palm meal and bean hulls are treated by adopting a bacterium-enzyme synergistic fermentation mode, the method is simple and convenient, not only are the antibacterial ability and the organic acid content of the palm meal and the bean hulls greatly improved, but also the palatability of the feed is improved, and it is possible to make palm meal and bean hulls into feed.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to Pediococcus lactis DY15 and its application, feed and its preparation method. Background technique [0002] Lactic acid bacteria is a general term for a class of Gram-positive, non-spore-forming bacteria whose main metabolite is lactic acid. As a living microorganism, lactic acid bacteria play an important role in the health of humans and animals. Their antibacterial and probiotic properties are used in many fields such as food fermentation, poultry and animal husbandry, medical care and industrial production of lactic acid. Lactic acid bacteria are also Then it became the key research object. Among them, the health effect of Pediococcus lactis is also used to treat intestinal infection and inflammatory bowel disease, and the addition of lactic acid bacteria in the poultry and livestock industry has the effect of antibiotics, which enriches the nutrition of feed and maintains the ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/12A23K10/14A23K10/33A23K10/37C12R1/01
CPCC12N1/20A23K10/12A23K10/14A23K10/37A23K10/33A23V2400/413Y02P60/87
Inventor 邓禹卢艳波侯刚杨小雁马忠玛刘耀新耿嘉宝王敏
Owner SHANDONG BOHI INDUSTRY CO LTD
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