Composition for antagonizing helicobacter pylori
A Helicobacter pylori and composition technology, applied in the field of antagonizing Helicobacter pylori compositions, can solve problems such as unsatisfactory application effects of Helicobacter pylori, antibiotic resistance, weak bactericidal effect of Helicobacter pylori, etc., and achieve Effect without adverse reaction
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Embodiment 1
[0029] Screening and identification of Lactobacillus plantarum HH-LP78 strain
[0030] 1. Strain screening
[0031] Take naturally fermented sauerkraut mash, carry out aseptic operation on an ultra-clean workbench, dip a ring of fermented mash with an inoculation needle, transfer to MRS agar solid medium, carry out streaking operation, and cultivate at 36°C to 38°C After 24 to 48 hours, pick a single colony and check for bacteria with a microscope. If the colony is impure, repeat the above-mentioned streaking operation until there is no miscellaneous bacteria in the single colony microscopic examination. The bacterial colony is white, occasionally light yellow or dark yellow, with a smooth surface, a raised center, and a round shape. The shape of the bacteria is a straight rod with round ends, usually 0.8-1.0 μm × 4.0-7.0 μm, single, paired or short chain.
[0032] The MRS agar solid medium is composed of glucose 2%, peptone 1%, beef extract 1%, yeast extract 0.5%, sodium a...
Embodiment 2
[0040] Preparation of dry powder of Lactobacillus plantarum HH-LP78:
[0041] The Lactobacillus plantarum is fermented and cultivated to obtain a fermented liquid, and the fermented liquid is centrifuged, emulsified, freeze-dried, and pulverized to prepare the Lactobacillus plantarum HH-LP78 active cell dry powder for subsequent use;
[0042] The fermentation medium is MRS liquid medium, which consists of 2% glucose, 1% peptone, 1% beef extract, 0.5% yeast extract, 0.5% sodium acetate, 0.25% diammonium hydrogen citrate, and 0.2% dipotassium hydrogen phosphate , magnesium sulfate 0.058%, manganese sulfate 0.025%, Tween-80 0.1%, pH6.8~7.0. The freeze-drying protective agent is 50% of skimmed milk powder, 30% of maltodextrin and 20% of lactose according to the percentage of solid content. The dry weight of the protective agent accounts for 20-80% by weight of the dry weight of the bacteria.
[0043] Preparation of mixed dry powder of dead Lactobacillus plantarum HH-LP78 and its m...
Embodiment 3
[0047] Preparation of roselle plant essential oil components: Roselle plant essential oils are extracted from roselle plant seeds and calyxes by traditional methods, and the plant volatile oils are coated with coating materials to obtain solid state roselle plant essential oil components; The dry weight of the coating material accounts for 25%-75% by weight of the plant essential oil; the coating material is 90% of beta-cyclodextrin and 10% of gelatin according to the percentage of solid content.
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