The invention discloses a preparation method of a museum environmental-protection humidifying agent, which adopts the following steps of A) adding konjac glucomannan and sodium carboxymethylcellulose in water to be blended and gelatinized; B) adding products of the step A) in crylic acid, sodium hydroxide, initiator and a cross-linking agent, adjusting the temperature to be at 55DEG C to 60 DEG C to react for 1-2h, and stoving the reaction products by using microwaves; and C) grinding stoved sample in the step B), sieving by using a 20-mesh sieve and a 40-mesh sieve, obtaining 20-40 mesh particles to add in buffered solution with the pH of 4-6, preheating for 5-10min in a water bath at the temperature of 50-60 DEG C, adding glucanase in certain proportion, conducting enzymolysis for 0.5-2h, boiling for 10min at the temperature of 100 DEG C for oxyreductase, conducting suction filtration on obtained products, and conducting microwave drying to obtain the museum environmental-protection humidifying agent. Dosage of the buffered solution is 20-30 times of that of the sample, and the dosage of glucanase is 0.5-2% of that of the sample. The preparation method is rapid in product humidity response, big in humidity ratio, simple in manufacture cost and convenient to manufacture.