The invention discloses a compound pesticide bactericidal composition containing trifloxystrobin. The compound pesticide bactericidal composition contains an effective component A and an effective component B in a mass ratio of 70:1-1:70. The component A is trifloxystrobin, and the component B is one of SYP-1620, mandipropamid, Mepanipyrim, Fluopicolide, and orysastrobin. The invention also discloses a preparation that is prepared from the composition and is suitable for agricultural use. Compared with a single trifloxystrobin agent, the compound bactericidal composition provided by the invention have obvious synergistic effects on downy mildew, rust disease, powdery mildew, leaf spot, scab, rice blast and other diseases, and improves the control effect. Also, the effective components of the bactericidal composition have mutually different action mechanisms, thus being in favor of overcoming disease resistance and delaying generation of pests' resistance to drugs. The field dosage can be greatly reduced, the environmental pollution and pesticide residue can be effectively reduced, and the production and use costs are reduced. At the same time, the bactericidal spectrum is expanded, thus being conducive to comprehensive prevention and control of crop diseases.