The invention relates to a bactericidal composition containing cyclostrobin and metalaxyl-methyl, the active ingredient of which is a binary compound of cyclostrobin and metalaxyl-methyl. Wherein the mass ratio of cyclostrobin and metalaxyl is 1-40:40-1, the mass ratio of active ingredients cyclostrobin and metalaxyl in the preparation is 1%-80%, and the rest are pesticides. The auxiliary ingredients that are allowed to be used and accepted, the formulations of the bactericidal composition of the present invention are emulsifiable concentrates, suspension concentrates, wettable powders, water dispersible granules, water emulsions, microemulsions, granules, and microcapsules. It can be used to control downy mildew, Phytophthora, Pythium, Wheat Scab, Cotton Fusarium Wilt, Banana Panama Disease, Rice Bakanae Disease, Watermelon Fusarium Wilt, etc.