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Lactobacillus acidophilus and application thereof

A technology of Lactobacillus acidophilus and microbial strains, applied in the direction of application, bacteria, microorganisms, etc., can solve the problems of unstable product quality, insufficient number of viable bacteria, low digestibility, etc., to enhance non-specific immune function and improve transformation Efficiency, promote digestion and absorption effect

Inactive Publication Date: 2011-10-12
北京龙科方舟生物工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The development and utilization of lactic acid bacteria products has become an active field in the feed industry, but there are still some problems to be solved, such as unstable product quality, insufficient number of viable bacteria, low digestibility and high cost.

Method used

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  • Lactobacillus acidophilus and application thereof
  • Lactobacillus acidophilus and application thereof
  • Lactobacillus acidophilus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1, Isolation and Identification of Lactobacillus acidophilus AI2021

[0065] Lactobacillus acidophilus AI2021 is extracted, isolated, screened and purified from pig gastrointestinal mucosa.

[0066] 1.1 Test material

[0067] Take healthy piglet gastrointestinal mucosa 5cm 2 (stomach, duodenum, jejunum, ileum, cecum, and colon), in a sterile cup, rinse the chyme on the mucosa with sterile saline, immerse the mucosa in a beaker containing 15 mL of HEPES buffer, and use forceps After shaking the mucosa back and forth for 5 minutes, the supernatant was obtained.

[0068] 1.2 Medium

[0069] 1.2.1 Medium of Rogosa SL agar

[0070] Tryptone 10g, beef extract 10g, yeast extract powder 5g, glucose 10g, arabinose 5g, sucrose 5g, sodium acetate 15g, sodium citrate 2g, potassium dihydrogen phosphate 6g, magnesium sulfate heptahydrate 0.58g, manganese sulfate tetrahydrate 0.25g, ferrous sulfate 0.03g, Tween-80 1mL, agar 13g, dilute to 1000mL with distilled water.

[00...

Embodiment 2

[0099] Embodiment 2, the research of the biological characteristic of lactobacillus fermentum

[0100] 1.1 Growth curve

[0101] MRS broth: peptone, 10g; Beef extract, 10g; Yeast extract powder, 5g; Dipotassium hydrogen phosphate, 2g; Diammonium citrate, 2g; Glucose, 20g; Magnesium sulfate heptahydrate, 0.58g; Manganese, 0.25g; Sodium acetate, 5g; Distilled water, 1000mL, 300mL MRS broth in a 500mL Erlenmeyer flask. Inoculate the Lactobacillus culture with 1% inoculum, take samples every 1h in the first 24h, and take samples at 28th, 32nd, 36th, 40th, 44th, 48h after 24h, take 1mL of the culture solution for gradient dilution, and in 10 -2 ~10 -6 Dilution Take 0.3mL dilution and spread evenly on MRS agar (pH5.2), make 3 parallel samples for each gradient, place the plate at 37°C, 5% CO 2 In the incubator, cultivate for 24 hours, take the dilution of 50-150 colonies in the plate for calculation, make 3 parallel samples for each gradient, and express the results as the averag...

Embodiment 3

[0134] Embodiment 3, the breeding of lactobacillus anti-escherichia coli

[0135] 1.1 Breeding of lactobacillus metabolites for the ability to inhibit Escherichia coli

[0136] Escherichia coli (K88, K99 and 987P) were purchased from China Veterinary Drug Control Institute. The preservation number of K88 in the China Medical Bacteria Collection Management Center is: CMCC44742, the preservation number of K99 in the China Medical Bacteria Collection Management Center is: CMCC44820, and the preservation number of 987P in the China Medical Bacteria Collection Management Center is: CMCC44317.

[0137] Lactobacillus: 71 strains of Lactobacillus acidophilus resistant to acid, high zinc, high copper and bile salts.

[0138] Medium: MacConkay medium.

[0139] Add about 15mL of MRS broth into the Hungates tube and fill with carbon dioxide to make sterile anaerobic MRS broth. Each tube was inoculated with 71 strains of acid-resistant, high-zinc-resistant, high-copper-resistant and bil...

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Abstract

The invention discloses a lactobacillus acidophilus and application thereof. The lactobacillus acidophilus is lactobacillus acidophilus and is collected in the China General Microbiology Center of Committee for Culture Collection of Microorganisms on 11th March, 2011; and the registered number of the lactobacillus acidophilus in the collection center is CGMCC No.4649. The lactobacillus acidophilus and a lactobacillus acidophilus preparation disclosed by the invention are mainly used as additives of animal fodder and can also be used for adjusting the microecological balance in animal intestines so that the product has a function of enhancing the specific immunity function to prevent and treat diseases and is also capable of providing trophic factors, accelerating digestion and absorption of nutrients, accelerating the animal growth and increasing the fodder conversion rate. Meanwhile, by screening the formulation, the cost of the preparation disclosed by the invention is low and the quality of the product is stable.

Description

technical field [0001] The invention relates to a strain of Lactobacillus acidophilus and its application, in particular to a Lactobacillus acidophilus preparation containing the Lactobacillus acidophilus, a feed, its preparation method and its use, and belongs to the field of biotechnology. Background technique [0002] Adding antibiotics to feed to promote animal growth has a history of more than 50 years and has made a significant contribution to the development of intensive animal husbandry. With the improvement of people's living standards and the increasing emphasis on health, people began to discover the increasingly prominent negative effects hidden behind the huge economic benefits brought by antibiotic feed additives, such as endogenous infections and secondary infections caused by antibiotics. Infection, the emergence of drug resistance, the destruction of normal intestinal flora, and residues in livestock products and the environment will pose serious threats to ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/23C12R1/19A23K10/18
Inventor 谯仕彦宋青龙丁修良张海燕
Owner 北京龙科方舟生物工程技术有限公司
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