Bismuth selenide nanometer material, preparation method and applications thereof

A nanomaterial, bismuth selenide technology, applied in the field of nanomaterial preparation and biomedicine, can solve the problems such as the application of bismuth selenide nanomaterials that have not been found, and achieve the effects of enhancing the body's immune function, controllable size, and low toxicity

Inactive Publication Date: 2013-09-11
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The results of searching domestic and foreign literature and patents on bismuth selenide nanomaterials show that before the application of the present invention, no reports on the application of bismuth selenide nanomaterials in tumor X-ray CT imaging have been found.

Method used

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  • Bismuth selenide nanometer material, preparation method and applications thereof
  • Bismuth selenide nanometer material, preparation method and applications thereof
  • Bismuth selenide nanometer material, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Bismuth selenide nanomaterials (Bi 2 Se 3 ) preparation

[0029] 0.25 g of polyvinylpyrrolidone (PVP) was fully dissolved in 10 mL of ethylene glycol, 0.0605 g of sodium selenite was fully dissolved in 8.75 mL of ethylene glycol, and 0.1130 g of bismuth nitrate pentahydrate was fully dissolved in 6.25 mL of ethylene glycol in diol. Then these three solutions were added into a 100 mL double-neck round bottom flask, subjected to ultrasonic treatment at room temperature, and mixed well. Then heated to 140 °C, rapid injection of hydroxylamine solution (0.6 mL of hydroxylamine dissolved in 5 mL of ethylene glycol). The solution immediately changed from milky white to dark brownish red, and was cooled to room temperature after 10 minutes of reaction. Finally, the solution was centrifuged at 60,000 rpm for 20 minutes, and washed three times with a mixed solution of acetone and water (300 mL of acetone and 60 mL of water) to obtain Bi 2 Se 3 nanomaterials. Characterized ...

Embodiment 2

[0031] Bismuth selenide nanomaterials (Bi 2 Se 3 ) stability and cytotoxicity

[0032] Bi 2 Se 3 Nanomaterials were placed in H 2 O, PBS, 1640 cell culture medium, and calf serum were used to make the final concentration 100 μg / ml, no precipitation was seen after standing at room temperature for one week, Bi in each solvent 2 Se 3 Uniform dispersion and good stability. HeLa cells were seeded in 96-well plates, and Bi 2 Se 3 Add it into the well plate to form a concentration gradient, and add the culture medium at the same time, add the MTT dye after 24 h, remove the MTT solution after 4 h, add 150 μL of dimethyl sulfoxide (DMSO), shake on the shaker at low speed for 10 min. Finally, the OD value was read at 570 nm on a microplate reader. The result is as figure 2 As shown, it can be seen that even a high concentration of Bi 2 Se 3 It is also basically non-toxic to cells.

Embodiment 3

[0034] Bi at the same molar concentration 2 Se 3 Comparison of CT values ​​between nanomaterials and iopamidol

[0035] Iopamidol is a CT contrast agent widely used clinically. Prepare Bi with the same molar concentration (calculated as Bi and I) 2 Se 3 The nanomaterials and iopamidol solution were placed in a 96-well plate, and then scanned by a 320-row Aquilion One computerized tomography scanner, and image analysis and CT value calculations were performed using the supporting CT software. The result is as image 3 As shown, at the same molar concentration, Bi 2 Se 3 The CT value (Hounsfield units, HU) of nanomaterials was significantly stronger than that of iopamidol.

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Abstract

The present invention discloses a bismuth selenide nanometer material, a preparation method and applications thereof. The preparation method comprises: dissolving a bismuth salt, a selenium-containing compound and a stabilizer in a solvent, carrying out an ultrasonic treatment, uniformly mixing, heating to a temperature of 80-200 DEG C, adding a reducing agent, cooling to a room temperature after completing a reaction, and carrying out centrifugation separation washing three times to obtain the bismuth selenide nanometer material with a diameter of 20-200 nm. The preparation method has characteristics of simple operation, less time consuming, low energy consumption and easy scalization. The prepared particles have a uniform particle size and good dispersity. With application of the bismuth selenide nanometer material as a living body X-ray CT imaging contrast agent, a significant imaging effect is provided compared with imaging effects of the traditional contrast agents such as an iodine reagent iopamidol and the like so as to provide important application prospects in the future tumor imaging field.

Description

technical field [0001] The invention belongs to the fields of nanomaterial preparation and biomedicine, and in particular relates to a bismuth selenide nanomaterial and a preparation method and application thereof. Background technique [0002] Cancer is a disease in which the mechanism of cell growth and proliferation is abnormal, and it will locally invade the surrounding normal tissues, and even transfer to other parts of the body through the internal circulatory system or lymphatic system, resulting in serious consequences. According to the statistics of the World Health Organization (WHO), in 2008, 7.6 million people died of cancer all over the world, accounting for about 13% of the total number of deaths. Moreover, the death toll is predicted to rise to 12 million by 2030. The dramatic increase in cancer deaths is a reminder of the urgent need for new, more effective diagnostic methods. [0003] Although traditional tumor diagnostic reagents have made gratifying prog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B19/04A61K49/04B82Y30/00
Inventor 杨黄浩
Owner FUZHOU UNIV
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