Preparation method of chitosan-based drug-loaded composite antibacterial microfiber membrane

Abstract

The invention provides a preparation method of a chitosan-based drug-loading composite antibacterial superfine fiber membrane. The preparation method comprises the following steps: S1, adding antibacterial medicine powder into an acetic acid aqueous liquid with mass fraction of 2-90%, stirring by a magnetic stirrer to fully dissolve, weighing chitosan and polyoxyethylene powder into the liquid, and stirring to obtain a spinning liquid; and S2, adding the spinning liquid into an unfolder for electrostatic spinning, putting the obtained electrostatic spinning membrane in a drier holding a 25% glutaraldehyde aqueous liquid for crosslinking for 12-36 hours, drying, and finally obtaining the chitosan-based drug-loading composite antibacterial superfine fiber membrane, wherein the diameter of the fiber is 150-600nm. The electrostatic spinning superfine fiber prepared by the invention has the characteristics of good adhesion, flexibility, good antibacterial performance, hemostasis, percolate absorption, healing and the like. By designing the compounding proportion of chitosan and antibacterial drug, the membrane can satisfy different anti-infection demands, wound nursing of different types and surgical dressing field.

Description

technical field [0001] The invention belongs to the technical field of preparation of composite nanofiber wound dressings, and in particular relates to an antibacterial superfine fiber film for medical dressings and a preparation method thereof. Background technique [0002] The incidence of chronic wounds such as non-healing leg ulcers, diabetic feet and decubitus (pressure sores) is exacerbated by an aging global population and increasing prevalence of obesity. Traditional dressings have little effect on treating such chronic wounds, causing great physical and mental suffering to patients. Chronic and complex wounds are difficult to heal, have a high rate of wound infection, and even lead to complications such as death and amputation in severe cases, and are regarded as a huge challenge in the global medical field. The emergence of high-end dressings undoubtedly brings hope for healing and pain relief to patients with chronic wounds. At present, high-end dressings mainly...

AI Technical Summary

Problems solved by technology

The patent of publication number CN102074703A discloses an antibacterial wound dressing containing silver fibers, which can continuously release silver ions and provide long-term effective antibacterial function; the patent of publication number CN101297976A has prepared a silver / chitosan antibacterial type Nanofiber membrane, after antibacterial test, the fiber membrane also has a high lethality to Escherichia coli, Staphylococcus aureus, etc. after 24 hours; the patent with publication number CN102392347A introduces a silver-containing hydrogel as the core layer. The preparation method of the composite fiber with long filament as the shell layer, in order to solve the current problem that nano-silver is easily oxidized when exposed to the air, and is easy to be sealed inside; the patent of publication number CN101062426A discloses the use of chitosan / polyvinyl alcohol / water-soluble Antibacterial drugs are used as raw materials and electrospinning is used to prepare biological dressings. The application of polyvinyl alcohol is affected by its molecular weight and degree of polymerization, which produces varying degrees of toxicity to tissues. In addition, its own inhibition of bacteria remains to be seen. Yu further confirmed; the patent publication number CN102266582A discloses a preparation method of a biodegradable nanofiber medical dressing loaded with drugs, and the porous nanofiber membrane is soaked in a therapeutic drug solution, and the prepared dressing can absorb tissue exudation Liquid, keep moist around the wound, and have the advantages of antibacterial, hemostasis, and beneficial to the growth and regeneration of wound tissue; the patent of publication number CN102210885A discloses the composite material of chitosan lactate and hyaluronic acid as the base material, adding Antibiotics, a method for preparing a sponge wound dressing with high air permeability, high absorbency and moisture retention by using a freeze-drying process; the patent of publication number CN1579559A adopts Co gamma-ray or high-energy electron beam ray radiation cross-linking to prepare chitosan / polyvinyl alcohol drug-loaded water Gel dressing has the characteristics of antibacterial properties, high water content, good mechanical strength, and good air permeability, but it is costly, dangerous, and easy to damage the molecular structure of chitosan, polyvinyl alcohol and drugs; the composite polyethylene prepared by Marziyeh Jannesari et al. Alcohol / polyvinyl acetate nanofiber pads are used as wound dressings, which can control the release of moxifloxacin hydrochloride, and use the high porosity of nanofibers to improve the absorbency of wound dressings; PLA)-polyethylene glycol (PEG) copolymer loaded with fluoroquinolone antibiotics can effectively avoid the occurrence of sudden drug release in wound dressings at the initial stage of use

[0004] In the current research on loading antibacterial agents, most of them focus on the research and application of silver-loaded antibacterial dressings, and there are few reports on drugs loaded with organic antibacterial agents. However, the negative biological effects of nano-silver on the human body, especially long-term frequent use Or chronic toxicity when used in large quantities is not very clear

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