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Phoma endophytic fungus and application thereof

A technology of endophytic fungi and cauline fungus, applied in applications, fungi, microorganisms, etc., can solve the problem of unpublished research reports, and achieve the effect of improving resistance, delaying symptoms of salt damage, and promoting growth

Inactive Publication Date: 2015-01-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have also been research reports on the use of endophytic fungi to improve the salt resistance of soybeans, but no research report has been published on the role of endophytic fungi in improving the salt tolerance and salt resistance of rice and wheat

Method used

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  • Phoma endophytic fungus and application thereof
  • Phoma endophytic fungus and application thereof
  • Phoma endophytic fungus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The separation, purification and identification of embodiment 1 strain KDZ-5

[0032] 1.1 Isolation and purification of bacterial strain KDZ-5

[0033] 1.1.1 Culture medium preparation

[0034] ① Potato dextrose agar medium (potato dextrose agar, PDA): 200g potato (peeled), 20g glucose, 20g agar, add distilled water to make up to 1000mL; Bacteria 20min).

[0035] ②Water agar medium (water agar, WA): 15g agar, add tap water to make up to 1000mL; then carry out routine high-temperature sterilization (1.1 atmosphere, 121℃ for 20min).

[0036] 1.1.2 Isolation and purification of bacterial strains

[0037] The KDZ-5 bacterial strain of the present invention is obtained in the laboratory according to the following isolation and culture conditions, including:

[0038]The plant samples of Sophora sophora were collected in the saline-alkali land in Aksu area, Xinjiang, China. After the plant samples were kept fresh, they were brought back to the laboratory. The plant samples...

Embodiment 2

[0079] Embodiment 2 KDZ-5 improves the test of rice seedling salt tolerance effect

[0080] 2.1 Culture medium preparation

[0081] ① Potato dextrose agar medium (potato dextrose agar, PDA): same as Example 1.

[0082] ②MS medium: potassium nitrate 1900mg / L, ammonium nitrate 1650mg / L, magnesium sulfate 370mg / L, potassium dihydrogen phosphate 170mg / L, calcium chloride 440mg / L, manganese sulfate 22.3mg / L, zinc sulfate 8.6mg / L , boric acid 6.2mg / L, potassium iodide 0.83mg / L, sodium molybdate 0.25mg / L, copper sulfate 0.025mg / L, cobalt chloride 0.025mg / L, ferrous sulfate 27.8mg / L, Na 2 EDTA 37.3mg / L, glycine 2.0mg / L, thiamine hydrochloride 0.1mg / L, pyridoxine hydrochloride 0.5mg / L, niacin 0.5mg / L, inositol 100mg / L, 30g sucrose, agar powder 7g, h 2 O dilute to 1L; pH 5.6-5.8.

[0083] 2.2 Cultivation of strain KDZ-5

[0084] Use a sterile toothpick to pick up the mycelial mass of the strain KDZ-5 and place it on a PDA medium plate, seal it with a parafilm, place it in a constan...

Embodiment 3

[0097] Embodiment 3KDZ-5 improves the hydroponic experiment of wheat seedling salt tolerance

[0098] 3.1 Culture medium and solution preparation

[0099] ① Potato dextrose agar medium (potato dextrose agar, PDA): same as Example 1.

[0100] ② Potato dextrose liquid medium (potato dextrose liquid, PBA): PDA medium without agar.

[0101] ③Hoagland nutrient solution: calcium nitrate tetrahydrate 945mg / L, potassium nitrate 506mg / L, ammonium nitrate 80mg / L, potassium dihydrogen phosphate 136mg / L, magnesium sulfate 493mg / L, iron salt solution 2.5mL, trace elements Solution 5mL, pH=6.0.

[0102] ④ Iron salt solution: 2.78g of ferrous sulfate heptahydrate, 3.73g of disodium edetate, 500mL of distilled water, pH=5.5.

[0103] ⑤ Trace element solution: potassium iodide 0.83mg / L, boric acid 6.2mg / L, manganese sulfate 22.3mg / L, zinc sulfate 8.6mg / L, sodium molybdate 0.25mg / L, copper sulfate 0.025mg / L, cobalt chloride 0.025 mg / L.

[0104] 3.2 Preparation of wheat seedlings

[0105] ...

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Abstract

The invention discloses a strain of phoma endophytic fungus and application thereof. The phoma endophytic fungus is named as (phoma sp.) KDZ-5 and has a preservation number of CCTCC No.M 2014162. The phoma endophytic fungus can be applied to improvement of rice or wheat salt resistance. The invention for the first time fins that the (phoma sp.) KDZ-5 can improve the resistance of rice and wheat to salt stress. Tests indicate that after establishment of a co-culture system from the KDZ-5 strain and rice seedlings, the KDZ-5 strain can significantly promote the growth of rice seedlings on a salted MS plate; under nutrient solution water culture and soil pot culture conditions, the KDZ-5 strain can be subjected to establishment of a co-culture system together with wheat seedlings, then the KDZ-5 strain can significantly slow down or delay salt injury symptoms of wheat in saline-alkali soil, and at the same time the biomass accumulation of wheat is greatly increased.

Description

technical field [0001] The invention belongs to the field of utilization of microbial germplasm resources, and in particular relates to an endophytic fungus of the genus Phoma and its application. Background technique [0002] Soil salinization reduces the productivity of arable land and is a very important abiotic stress in food production. It causes serious losses to the world's food production every year and seriously affects the ecological balance of the region. Currently, more than 800 million hectares of land around the world suffer from land salinization, accounting for 6% of all land area. This problem is especially acute in regions with arid, semi-arid and Mediterranean-type climates. In recent years, irrational cultivation and irrigation, as well as environmental pollution and destruction of ecological balance are the main causes of salinization. The problem of soil salinization in my country is very serious. Saline-alkali land is distributed in a wide range, lar...

Claims

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Application Information

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IPC IPC(8): C12N1/14C09K17/00C12R1/645C09K101/00
CPCC09K17/00C09K2101/00C12N1/145C12R2001/645
Inventor 王洪凯陈亚平林福呈
Owner ZHEJIANG UNIV
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