Production method of high-protein feed containing arginine
The technology of a high-protein feed and a production method is applied to the production field of high-protein feed rich in arginine, which can solve the problems of animal weight loss, restricted utilization, and reduced nutritional value of dietary protein, so as to reduce the content of anti-nutritional factors. , The effect of reducing the probability of contaminating bacteria and shortening the solid-state fermentation time
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Embodiment 1
[0024] The high-protein feed production method rich in arginine of the present invention, comprises the following steps:
[0025] The first step, liquid fermentation
[0026] The Corynebacterium glutamicum numbered CICC 20191 on the beef extract peptone was made into a suspended bacterial liquid with 200ml sterile saline, and inoculated into the liquid fermentation medium (filling volume 60%), medium composition: glucose 120g / L, (NH 4 ) 2 SO 4 30g / L, corn steep liquor 20g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 0.5 g / L, biotin 8×10 -5 g / L, CaCO 3 30 g / L; pH7.0, sterilized at 0.12 MPa for 20 min. Culture conditions: tank pressure 0.1 Mpa, stirring speed 230 r / min, ventilation rate 1:0.25, culture temperature 30°C, fermentation time 48 h; use 200ml of Candida utilis numbered CICC 1801 on YPD medium Sterile physiological saline was used to make a suspended bacterial liquid, and when the acid production rate of the Corynebacterium glutamicum fermentation liquid reached ...
Embodiment 2
[0038] The high-protein feed production method rich in arginine of the present invention, comprises the following steps:
[0039] The first step, liquid fermentation
[0040] The mixed fermentation of Corynebacterium glutamicum and Candida utilis was the same as in Example 1.
[0041] The slanted seeds of Bacillus natto with the number CICC23413 were inoculated in a 500ml eggplant-shaped bottle filled with peptone beef extract medium, and the first-grade solid seeds of Bacillus natto were made into a suspended bacterial solution with 200ml sterile physiological saline, and inoculated into Fermentation culture in 2m3 fermenter (60% of seed tank media filling volume), medium composition: 2.0% soybean flour, 1.5% corn flour, 0.4% magnesium sulfate, 0.05% manganese sulfate, 1.0% sodium chloride; culture conditions : Tank pressure 0.05 Mpa, stirring speed 190 r / min, ventilation rate 1:0.8, culture temperature 35°C, fermentation 20 h.
[0042] The second step, the preparation ...
Embodiment 3
[0052] The high-protein feed production method rich in arginine of the present invention, comprises the following steps:
[0053] The first step, liquid fermentation:
[0054] The preparation method of Corynebacterium glutamicum (CICC 20191) and Candida utilis mixed bacteria liquid is the same as in Example 1; the preparation method of Lactobacillus plantarum fermentation liquid is the same as in Example 1; the preparation method of Bacillus natto fermentation liquid is the same as in Example 1 2 same;
[0055] The second step, the preparation of mixed fermented mash:
[0056] Get 30 parts of the Corynebacterium glutamicum and Candida utilis mixed bacteria fermentation liquid obtained in the first step, 10 parts of Lactobacillus plantarum seed liquid, 10 parts of Bacillus natto seed liquid and mix evenly, with Ca(OH) 2 Adjust the pH to 7.0 to obtain fermented mash;
[0057] The third step, solid state fermentation:
[0058] According to weight ratio, 150 parts of dehulled ...
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