Production method of high-protein feed containing arginine

The technology of a high-protein feed and a production method is applied to the production field of high-protein feed rich in arginine, which can solve the problems of animal weight loss, restricted utilization, and reduced nutritional value of dietary protein, so as to reduce the content of anti-nutritional factors. , The effect of reducing the probability of contaminating bacteria and shortening the solid-state fermentation time

Inactive Publication Date: 2015-09-23
河南双成生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Their shortage limits the utilization of other amino acids in the diet in livestock and poultry, reducing the nutritional value of the protein in the whole diet
[0003] Arginine is also known as a semi-essential amino acid. Although it can be synthesized endogenously through the decomposition of protein in the animal body or the conversion of other amino acids (glutamic acid and citrulline, etc.) in the body, the amount of synthesis usually cannot meet the normal physiological needs of the animal body. Or mainly rely on the animal to absorb from the basal diet
When arginine is lacking in the diet, it can cause animal weight loss, low feed conversion efficiency, etc.
The content of arginine in agricultural and sideline products such as soybean

Method used

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  • Production method of high-protein feed containing arginine
  • Production method of high-protein feed containing arginine
  • Production method of high-protein feed containing arginine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The high-protein feed production method rich in arginine of the present invention, comprises the following steps:

[0025] The first step, liquid fermentation

[0026] The Corynebacterium glutamicum numbered CICC 20191 on the beef extract peptone was made into a suspended bacterial liquid with 200ml sterile saline, and inoculated into the liquid fermentation medium (filling volume 60%), medium composition: glucose 120g / L, (NH 4 ) 2 SO 4 30g / L, corn steep liquor 20g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 0.5 g / L, biotin 8×10 -5 g / L, CaCO 3 30 g / L; pH7.0, sterilized at 0.12 MPa for 20 min. Culture conditions: tank pressure 0.1 Mpa, stirring speed 230 r / min, ventilation rate 1:0.25, culture temperature 30°C, fermentation time 48 h; use 200ml of Candida utilis numbered CICC 1801 on YPD medium Sterile physiological saline was used to make a suspended bacterial liquid, and when the acid production rate of the Corynebacterium glutamicum fermentation liquid reached ...

Embodiment 2

[0038] The high-protein feed production method rich in arginine of the present invention, comprises the following steps:

[0039] The first step, liquid fermentation

[0040] The mixed fermentation of Corynebacterium glutamicum and Candida utilis was the same as in Example 1.

[0041] The slanted seeds of Bacillus natto with the number CICC23413 were inoculated in a 500ml eggplant-shaped bottle filled with peptone beef extract medium, and the first-grade solid seeds of Bacillus natto were made into a suspended bacterial solution with 200ml sterile physiological saline, and inoculated into Fermentation culture in 2m3 fermenter (60% of seed tank media filling volume), medium composition: 2.0% soybean flour, 1.5% corn flour, 0.4% magnesium sulfate, 0.05% manganese sulfate, 1.0% sodium chloride; culture conditions : Tank pressure 0.05 Mpa, stirring speed 190 r / min, ventilation rate 1:0.8, culture temperature 35°C, fermentation 20 h.

[0042] The second step, the preparation ...

Embodiment 3

[0052] The high-protein feed production method rich in arginine of the present invention, comprises the following steps:

[0053] The first step, liquid fermentation:

[0054] The preparation method of Corynebacterium glutamicum (CICC 20191) and Candida utilis mixed bacteria liquid is the same as in Example 1; the preparation method of Lactobacillus plantarum fermentation liquid is the same as in Example 1; the preparation method of Bacillus natto fermentation liquid is the same as in Example 1 2 same;

[0055] The second step, the preparation of mixed fermented mash:

[0056] Get 30 parts of the Corynebacterium glutamicum and Candida utilis mixed bacteria fermentation liquid obtained in the first step, 10 parts of Lactobacillus plantarum seed liquid, 10 parts of Bacillus natto seed liquid and mix evenly, with Ca(OH) 2 Adjust the pH to 7.0 to obtain fermented mash;

[0057] The third step, solid state fermentation:

[0058] According to weight ratio, 150 parts of dehulled ...

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Abstract

The invention discloses a production method of high-protein feed containing arginine. The production method comprises the following steps: performing liquid fermentation with corynebacterium glutamicum, candida utilis, lactobacillus plantarum and bacillus natto; mixing fermented mash rich in metabolic products with mash rich in thalluses, then mixing the mixture with soybean meal and the like in proportion, and performing solid state fermentation; finally drying products, and packaging the dried products so as to obtain final products containing crude protein of which the content is higher than 50% and arginine of which the content is higher than 4.0%. The production method disclosed by the invention has the advantages that the technology is simple, mixed fermentation is directly performed on the fermented mash and solid materials, the microbial metabolism product namely the arginine is utilized, so that the time of the solid state fermentation is greatly shortened, and the probability of infectious microbes infection in the solid state fermentation process of raw materials is reduced; the energy consumption is low, and waste is not discharged in the fermentation process; according to feed raw materials prepared by the production method, the content of anti-nutritional factors in a plant meal group is reduced, the content of the arginine is higher than 4.0%, the additive amount of exogenous arginine can be reduced or replaced, and the cost of the feed is reduced.

Description

technical field [0001] The invention relates to the field of feed production, in particular to a method for producing high-protein feed rich in arginine. Background technique [0002] Agricultural and sideline products such as soybean meal, cotton meal and peanut meal have a large output in the grain and oil industry, and are generally used as protein raw materials for animal feed, and are widely used in feed processing, livestock and poultry, and aquaculture industries. However, this kind of cake belongs to vegetable protein and contains a large number of anti-nutritional factors, such as trypsin inhibitors, soybean antigenic protein, uric acid, plant lectins, non-starch polysaccharides, etc., which affect the digestion and absorption of feed by animals. Therefore, in production, it is necessary to effectively remove anti-nutritional factors in cakes and improve feed utilization; , a low-cost substitute for high-quality feed protein raw materials. High-quality feed prote...

Claims

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Application Information

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IPC IPC(8): A23K1/02A23K1/14A23K10/33
CPCY02P60/87
Inventor 曹胜炎杨森宋安东王风芹谢慧李伟
Owner 河南双成生物科技有限公司
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