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Bacillus amyloliquefaciens subsp.plantarum and application thereof to scagassum biodegradation

A technology of bacillus and bacillus plants, applied in the field of microorganisms, can solve the problems of waste pollution of the environment and high energy consumption

Active Publication Date: 2017-02-15
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In industry, mechanical pulverization and chemical degradation are often used to degrade sargassum, but there are disadvantages such as high energy consumption and waste pollution.

Method used

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  • Bacillus amyloliquefaciens subsp.plantarum and application thereof to scagassum biodegradation
  • Bacillus amyloliquefaciens subsp.plantarum and application thereof to scagassum biodegradation
  • Bacillus amyloliquefaciens subsp.plantarum and application thereof to scagassum biodegradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Isolation of Bacillus HB12274 CGMCC No.12455

[0021] Sediment samples were collected from the mangrove forest in Dongzhai Port, Hainan Province by using the 5-point sampling method. Dissolve 10g of the sample in 90ml of sterile water, shake it in a constant temperature shaker at 37°C and 200r / min for 30min, let it stand for 5min, and prepare 10 -2 ~10 -4 series of soil suspensions. Use 80°C water bath for 20 minutes to kill non-spore-forming bacteria, draw 0.1ml of the series suspension, spread it on the bacteria isolation medium, and place it in a 37°C incubator for upside-down cultivation. When the colony grows, according to the phenotype characteristics such as colony shape, color, edge state, transparency, and surface dry and wet state, pick a single colony and purify it by streaking for 2 to 3 times until pure culture is obtained. After initial deduplication, number and save.

Embodiment 2

[0022] Example 2 Species Identification of Bacillus HB12274 CGMCC No.12455

[0023] (1) Morphology and culture characteristics

[0024] Streak culture of the strain on wort nutrient agar medium, the strain grows rapidly, the colony diameter is 3-5mm, round, with irregular edges, matte, uneven, milky white, opaque; the bacteria are long rod-shaped, Gram The result of Shi's staining was positive, producing spores, and one end was enlarged ( figure 1 ).

[0025] (2) Physiological and biochemical characteristics

[0026] Strain HB12274 can utilize glucose, sorbitol, maltose, sucrose, cellobiose, galactose, mannitol, fructose, lactose and arabinose. Gram staining, VP determination, contact enzyme test positive, MR test negative, can reduce nitrate, can make gelatin liquefaction.

[0027] (3) 16S rDNA sequence determination and phylogenetic analysis

[0028] The 16S rDNA sequence 1450bp of HB12274 was obtained by PCR amplification and sequencing, and the nucleotide sequence acc...

Embodiment 3

[0030] Embodiment 3: the preparation of strain HB12274 CGMCC No.12455 liquid bacterial agent

[0031] (1) Strain activation: the Bacillus amyloliquefaciens plant subsp. HB12274 was inoculated on a nutrient agar solid medium, and cultured at 37° C. for 24 hours. Medium: 10.0g peptone, 3.0g beef extract powder, 18.0g agar powder, 16.0g sodium chloride, 1000mL, pH7.5, sterilized at 121°C for 20min.

[0032] (2) Liquid culture: Inoculate the activated bacterial strain into a nutrient agar liquid medium, shake and culture at 37° C. and 200 r / min for 24 hours to prepare a seed liquid.

[0033](3) Fermentation culture: the seed liquid was inoculated into nutrient agar liquid medium at a volume ratio of 3%, and vibrated at 37° C. and 200 r / min for 72 hours to obtain a liquid bacterial preparation of Bacillus amyloliquefaciens subsp. HB12274.

[0034] According to the above steps, the liquid microbial agent of Bacillus amyloliquefaciens subspecies HB12274 is prepared.

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Abstract

The invention discloses a bacillus HB12274 and an application thereof to the scagassum biodegradation, and belongs to the technical field of microorganisms. The bacillus is bacillus amyloliquefaciens subsp.plantarum HB12274 and is separated from a bottom sediment sample of Mangrove at Dongzhai Harbor in Hainan. The strain is collected in China General Microbiological Culture Collection Center (CGMCC) in May 16, 2016 and has a collection number of CGMCC No.12455. The bacillus HB12274 has the activity of degrading algin, cellulose and starch, and can effectively degrade scagassum. The bacillus HB12274 and a fermentation liquor thereof can serve as new microbial resources used for the scagassum biodegradation, and have good application and development prospects in the aspects of scagassum processing and utilization.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Bacillus amyloliquefaciens plant subspecies HB12274 and its application in biodegradation of Sargassum algae. Background technique [0002] Sargassum is a common type of large brown algae, belonging to Phaephyta, Sargassaceae, and Sargassum. Most of them are warm-water species and are widely distributed in warm and warm waters. Sargassum has high economic value and has great development potential in agriculture and industry. It is an important fertilizer, feed and industrial raw material. The cell wall of Sargassum algae is mainly composed of cellulose and alginate, which has a stable structure and is difficult to be destroyed. Degradation of cellulose and alginate in the cell wall of Sargassum is the key to efficiently degrade and utilize Sargassum. In industry, mechanical pulverization and chemical degradation are often used to degrade Sargasso algae, but...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/88C12N9/26C12N9/42C12P19/14C12P19/00C12R1/07
CPCC12N9/2411C12N9/2437C12N9/88C12P19/00C12P19/14C12N1/205C12R2001/07
Inventor 朱军邹潇潇黄惠琴鲍时翔
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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