Biological chip and preparation method thereof

Abstract

The invention relates to a biological chip and a preparation method thereof. The biological chip comprises a substrate and patterns on the surface of the substrate. The substrate is prepared from an anti-adhesion material, and the patterns contain an adhesive material used for adhering cells; or the substrate contains the adhesive material used for adhering the cells, and the patterns are preparedfrom the anti-adhesion material, wherein the anti-adhesion material is zinc or magnesium or zinc alloy or magnesium alloy. Because the anti-adhesion substrate or the anti-adhesion patterns are formedby using zinc or magnesium or zinc alloy or magnesium alloy, the anti-adhesion substrate or the anti-adhesion patterns adhere fewer cells, but the adhesive material can adhere a lot of cells, and patterned growth of the cells is achieved. By means of zinc or magnesium or zinc alloy or magnesium alloy, the characteristic that high molecular materials and biomacromolecules are not resistant to treatment is overcome, micrometer and nanometer adhesive pattered treatment can be directly conducted, the making process is simplified, and convenience is brought to the pattered making process; moreover, the property of zinc or magnesium or zinc alloy or magnesium alloy is not likely to be changed when zinc or magnesium or zinc alloy or magnesium alloy is physically or chemically treated, and the long-term anti-adhesion effect is good.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a biological chip and a preparation method thereof. Background technique [0002] Biochips are based on the principle of specific interactions between biomolecules, using different methods to immobilize biomolecules (such as oligonucleotides, cDNA, genomic DNA, polypeptides, antibodies, antigens, etc.) Biomolecular lattices formed on solid-phase transmitters such as sheets (beads), gels, and nylon membranes, so as to realize high-throughput and rapid detection of biological components such as DNA, RNA, polypeptides, and proteins, or to classify cell phenotypes Identification. Among them, the array of adherent cells prepared on the surface of the anti-cell adhesion material is a biochip that can control cell adhesion, also known as cell adhesion pattern. Cell adhesion patterns can be used for high-throughput and rapid cell phenotype detection, and can be applied to basic theo...

AI Technical Summary

Problems solved by technology

However, on the one hand, because the traditional anti-adhesion materials are generally some biomacromolecules or polymer materials with unstable structures, which are easily deformed or denatured by physical and chemical treatments, it is necessary to prepare biochips on a hard substrate (such as silicon). Sheets, glass slides, etc.) surface preparation to form a pattern area, and then perform anti-adhesion treatment on the substrate or transfer the pattern to the surface of the anti-adhesion material. The operation is complicated, which affects the accuracy of the patterning technology and limits the application range of the patterning technology.

On the other hand, most of the traditional anti-adhesion materials have unsatisfactory anti-adhesion effects and cannot maintain the long-term selective adhesion properties of cell adhesion patterns.

At present, PEG gel, the most long-lasting anti-adhesion material, can only achieve an anti-adhesion effect of 7 days to 30 days, and this material is poor in resistance to physical and chemical forces, and its application range is limited. It is necessary to find better tolerance Materials expand application range of patterning technology

[0005] In summary, traditional biochips are prone to denaturation due to physical or chemical treatment due to anti-adhesive materials, making patterning inconvenient, and the long-term anti-adhesive effect is poor.

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