Biochip and its preparation method

A biochip and polymer technology, applied in chemical instruments and methods, laboratory utensils, laboratory containers, etc., can solve the limitation of application range, the inability to maintain long-term selective adhesion of cell adhesion patterns, easy deformation or denaturation And other issues

Active Publication Date: 2021-02-02
MICROPORT SINICA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, on the one hand, because the traditional anti-adhesion materials are generally some biomacromolecules or polymer materials with unstable structures, which are easily deformed or denatured by physical and chemical treatments, it is necessary to prepare biochips on a hard substrate (such as silicon). Sheets, glass slides, etc.) surface preparation to form a pattern area, and then perform anti-adhesion treatment on the substrate or transfer the pattern to the surface of the anti-adhesion material. The operation is complicated, which affects the accuracy of the patterning technology and limits the application range of the patterning technology.
On the other hand, most of the traditional anti-adhesion materials have unsatisfactory anti-adhesion effects and cannot maintain the long-term selective adhesion properties of cell adhesion patterns.
At present, PEG gel, the most long-lasting anti-adhesion material, can only achieve an anti-adhesion effect of 7 days to 30 days, and this material is poor in resistance to physical and chemical forces, and its application range is limited. It is necessary to find better tolerance Materials expand application range of patterning technology
[0005] In summary, traditional biochips are prone to denaturation due to physical or chemical treatment due to anti-adhesive materials, making patterning inconvenient, and the long-term anti-adhesive effect is poor.

Method used

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  • Biochip and its preparation method

Examples

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preparation example Construction

[0155] Also, see Image 6 , the preparation method of the above-mentioned biochip according to one embodiment, comprising the following steps S110-S120.

[0156] S110, providing a substrate.

[0157] In one embodiment, the structure of the prefabricated biochip can be found in figure 1 , the substrate is an anti-adhesive substrate. The substrate is formed of an anti-adhesive material that resists cell adhesion, and the anti-adhesive material is zinc, magnesium, zinc alloy or magnesium alloy. Specifically, the substrate is, for example, a zinc plate, a magnesium plate, a zinc alloy plate or a magnesium alloy plate. The surface of the zinc plate, magnesium plate, zinc alloy plate or magnesium alloy plate is the anti-adhesive surface.

[0158] Alternatively, the base includes a substrate and an anti-sticking layer formed on the substrate, and the material of the anti-sticking layer is zinc, magnesium, zinc alloy or magnesium alloy. The substrate is, for example, a glass or a...

Embodiment 1

[0203] A zinc-magnesium alloy substrate with a thickness of 1mm (the mass percent of magnesium in the zinc alloy is 5%) is polished and pickled in a polishing solution. Specifically, the metal is immersed in the pickling solution, and the solvent of the pickling solution is Water, containing sulfuric acid (mass fraction concentration is 98%) 250mL / L, hydrochloric acid (mass fraction concentration is 37%) 20mL / L, nitric acid (mass fraction concentration is 68%) 20mL / L, pickling temperature is 25 ℃, time 5 to 100 seconds, and the final roughness of the anti-adhesive surface is 1 μm or less. Then placed in acetone, ultrasonicated three times, blown dry with nitrogen and baked at 120°C for 8 hours to make the anti-adhesive surface hydrophobic. Take it out and cool it down to room temperature, and spin-coat photoresist (Suzhou Ruihong, RZJ-3038) on its surface in a darkroom clean room. The substrate after homogenization was placed in an oven at 100°C for 20 minutes. Take it out a...

Embodiment 2

[0205] The zinc substrate with a thickness of 1 mm was polished and pickled in oxalic acid to remove surface impurities and make the anti-adhesive surface roughness 1 μm. Then placed in acetone, ultrasonicated three times, blown dry with nitrogen and baked at 110°C for 8 hours to make the anti-adhesive surface hydrophobic. According to the steps in Example 1, a gold micron pattern was prepared on a zinc-magnesium alloy substrate, and then a c-(RGD-fK)-SH (sulfhydryl group modified on RGD peptide) solution with a concentration of 25 μM was prepared and added dropwise to the gold micron pattern on the surface of the substrate. On patterning, a biochip with RGD micro-patterns that can selectively adhere to cells was obtained. In addition, RGD (arginine-glycine-aspartic acid) peptide, RGD peptide derivative (cyclic arginine-glycine-aspartic acid-phenylalanine-lysine Polypeptides—c-(RGD)-fK), YIGSR (tyrosine-isoleucine-glycine-serine-arginine polypeptides), RGD polypeptides, YIGSR...

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Abstract

The invention relates to a biological chip and a preparation method thereof. The biochip includes a substrate and a pattern on the surface of the substrate, the substrate is formed of an anti-adhesive material, and the pattern contains an adhesive material for adhering cells. Alternatively, the substrate contains an adhesion material for adhesion of the cells and the pattern is formed of an anti-adhesion material. The anti-adhesive material is zinc, magnesium, zinc alloy or magnesium alloy. Using zinc, magnesium, zinc alloy or magnesium alloy to form an anti-adhesive substrate or anti-adhesive pattern, the cells adhere less, while a large number of cells can adhere to the adhesive material, thereby realizing the patterned growth of cells. Zinc, magnesium, zinc alloys or magnesium alloys overcome the characteristics of high molecular materials and biological macromolecular materials that are not resistant to treatment, and can directly carry out micro- and nano-adhesion patterning treatment, simplify the production process, and facilitate patterning production, and zinc, Magnesium, zinc alloy or magnesium alloy is not easy to denature when encountering physical or chemical treatment, and has better long-term anti-adhesive effect.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a biological chip and a preparation method thereof. Background technique [0002] Biochips are based on the principle of specific interactions between biomolecules, using different methods to immobilize biomolecules (such as oligonucleotides, cDNA, genomic DNA, polypeptides, antibodies, antigens, etc.) Biomolecular lattices formed on solid-phase transmitters such as sheets (beads), gels, and nylon membranes, so as to realize high-throughput and rapid detection of biological components such as DNA, RNA, polypeptides, and proteins, or to classify cell phenotypes Identification. Among them, the array of adherent cells prepared on the surface of the anti-cell adhesion material is a biochip that can control cell adhesion, also known as cell adhesion pattern. Cell adhesion patterns can be used for high-throughput and rapid cell phenotype detection, and can be applied to basic theo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00
CPCB01L3/5027B01L3/502707B01L2300/08B01L2300/0887B01L2300/0893B01L2300/12B01L2300/161
Inventor 王玄陈陆李俊菲
Owner MICROPORT SINICA CO LTD
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