Heterotrophic nitrification-aerobic denitrification bacterium and application thereof

A technology of aerobic denitrification and heterotrophic nitrification, applied in the field of heterotrophic nitrification-aerobic denitrification bacteria, can solve the problems of high operating cost and complicated process of sewage treatment

Active Publication Date: 2021-12-03
ZHEJIANG UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a heterotrophic nitrifying-aerobic denitrifying Acinetobacter and its application in view of the shortcomings of high sewage treatment operating costs and cumbersome processes in the prior art.

Method used

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  • Heterotrophic nitrification-aerobic denitrification bacterium and application thereof
  • Heterotrophic nitrification-aerobic denitrification bacterium and application thereof
  • Heterotrophic nitrification-aerobic denitrification bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Screening and Identification of Hydrogen Titrification - Good Oxygen Nitrification Bacteria

[0028] Take 2 ~ 3G Hangzhou's bio-turned mud sample to add a tapered bottle containing 100 ml of sterile water, after 3 h on the shaker, use a pipette to take the liquid gun inoculated in a sterilized LB culture. In the group, 2 to 3d were cultured under 30 ° C, 180 rpm.

[0029] The above Lb medium: trypsin 10g, yeast powder 5g, sodium chloride 10g, distilled water to 1000 mL, natural pH; if solid medium is required, 1.5 to 2% agar.

[0030] In the test tube of the bacteria culture liquid, it is diluted in proportion, and the concentration of strains is obtained. -1 10 -2 10 -3 10 -4 10 -5 10 -6 10 -7 And 10 -8 The gradient of the bacteria suspension; and then used a plate coating method, 100 μL of 100 μL was taken from the LB solid medium from the LB solid medium, and the colonies were placed in a constant temperature incubator at a constant temperature in a 30 ° C, an...

Embodiment 2

[0038] Example 2: Identification of nitrification / denitrification capability of strain ZJB20129 on different nitrogen source media

[0039] Nitrification medium formulation: ammonium sulfate ((NH) 4 ) 2 SO 4 0.47 g, sodium butyric acid (C 4 Hide 4 NA 2 O 4 5.06g, magnesium sulfate (MGSO 4 0.1g, potassium phosphate (KH 2 PO4) 0.1g, 2 mL of trace element solution, distilled water to 1000 mL, pH 7.0-7.5; wherein the microelectric solution comprises: EDTA 50g, zinc sulfate (ZnSO) 4 · 7h 2 O) 3.92 g, calcium chloride (CaCl 2 5.5 g, four hydrocarbon chloride (MNCL 2 · 4h 2 O) 5.1g, seven water sulfate (FESO 4 · 7h 2 O) 5.0g, four water molybdate ammonium ammonium ((NH 4 6MO 7 O 24 · 4h 2 O) 1.1g, copper sulfate (Cuso) 4 · 5h 2 O) 1.6G, cobalt chloride (COCL) 2 · 6h 2 O) 1.6g, and disposable with distilled water to 1000 ml. Denitrification medium will ammonium sulfate in nitrification medium ((NH) 4 ) 2 SO 4 0.47g replacement with potassium nitrate (KNO 3 0.72g or sodium nitrite (nano ...

Embodiment 3

[0044] Example 3: Determination of Efficient Nitrification Conditions in ZJB20129

[0045] (1) Effect of Different Carbon Sources on ZJB20129 Ammonia Nitrogen Degradation Rate: Changes the carbon sources of nitrification medium in Example 2 as sodium acetate, sodium butyricate, sodium citrate, sucrose and glucose, fixed carbon source content of 1.5 g / L (with C), inoculated 2% ZJB20129 seed fluid, under conditions of 30 ° C, 160 rpm, cultured 36 h, determined its ammonia nitrogen degradation ability, Image 6 Indicated. It is shown that the ammonia nitrogen removal rate is 98.26% at a good acetate, and the ammonia nitrogen removal rate is 99.01% at a carbon source, which is much higher than the content of the same (in C), sodium citrate. As the clearance rate of the carbon source.

[0046] (2) Effect of Different Carbon Nitrogen Comparison of ZJB20129 Ammonia Nitrogen Degradation Rate: Changes the content of sodium nitrate medium in Example 2, so that C / N mass ratios in the medi...

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Abstract

The invention discloses a heterotrophic nitrification-aerobic denitrification bacterium and an application thereof. The heterotrophic nitrification-aerobic denitrification bacterium is preserved in the China Center for Type Culture Collection (CCTCC), the classification name is Acinetobacter indicus ZJB20129, the preservation number is CCTCC NO: M 2020965, and the preservation date is December 24, 2020. The heterotrophic nitrification-aerobic denitrification acinetobacter indicus strain B2-1 (Acinetobacter indicus strain B2-1) ZJB20129 can utilize sodium succinate and sodium acetate as carbon sources, has the optimal ammonia nitrogen removal effect which is 99.27% when being aerobically cultured for 36 hours under the conditions that the sodium succinate is the optimal carbon source, the C / N mass ratio is 15, the pH value is 8, the temperature is 35 DEG C and the rotating speed is 160rpm. Meanwhile, the strain has a very good removal effect on nitrate nitrogen and nitrite nitrogen.

Description

Technical field [0001] The present invention relates to the field of nitrogen-containing sewage treatment, in particular a heterotrophic nitrification-aerobic denitrification bacteria and its application thereof. Background technique [0002] The rapid development of industrial and agriculture and human production activities have brought serious pollution to the water environment. Nitrogen over-standard is the most common type of pollution in water pollution, which will cause eutrophication of water body, resulting in a large amount of algae, and water dissolving oxygen decreases, and aquatic organisms die, which not only endanger human water safety, but also seriously affects the ecosystem of water. Balance; additionally, the excess of nitrogen discharge can cause nitrogen balance to be destroyed, resulting in loss of self-cleaning in the water body. Therefore, the nitrogen-rich sewage needs to be discharged into the natural environment after demodifying the standard. [0003] B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/30C12R1/01C02F101/16
CPCC02F3/34C02F3/302C02F2101/16C02F2101/163C02F2101/166Y02W10/10
Inventor 薛亚平刘聪张剑峰柯霞郑裕国杨海本徐献忠
Owner ZHEJIANG UNIV OF TECH
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