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High-nitrogen compound microbial fertilizer as well as preparation method and application thereof

A compound microorganism and microbial fermentation technology, which is applied in the field of high nitrogen compound microorganism fertilizer and its preparation, can solve the problems of difficulty in unifying and coordinating culture temperature and culture period, difficult unification of culture temperature and culture time, and difficulty in guaranteeing the synergy of strains. To achieve the effect of shortened fermentation cycle, wide source of raw materials and low price

Pending Publication Date: 2022-01-28
SHANGHAI NONGLE BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the invention does not specify the specific ratio. If the selection is improper, antagonism may occur between the various strains, resulting in the inability of the strains to coexist or difficulty in exerting their own effects.
Moreover, the culture temperature of bacteria is generally 28-37°C, the culture temperature of yeast is 25-30°C, and the culture temperature of mold is 25-28°C. It is difficult to unify the culture temperature and culture time of different bacteria, and most fungi They are all molds, and molds are too broad, and it is difficult to coordinate the culture temperature and culture cycle, so the preparation is more complicated, and the synergy between strains is difficult to ensure, and it is difficult to achieve the effect of stable nitrogen fixation
There are also literatures disclosing another high-nitrogen compound microbial fertilizer and its preparation method. This technology completes microbial nitrogen fixation, humic acid activation, amino acid synthesis and organic fertilizer fermentation in one step during the biochemical reaction process, which greatly reduces production costs. The amount of urea in this fertilizer is relatively high (10-33%), and the raw materials also contain fish meal and soybean meal, and the fermentation time is as long as 15-25 days

Method used

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  • High-nitrogen compound microbial fertilizer as well as preparation method and application thereof
  • High-nitrogen compound microbial fertilizer as well as preparation method and application thereof
  • High-nitrogen compound microbial fertilizer as well as preparation method and application thereof

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preparation example Construction

[0029] The present invention also provides a preparation method of a microbial fermentation agent, the preparation method comprising the following steps: inoculating Paenibacillus polymyxa, Bacillus subtilis, and Paenibacillus jelly-like into the microbial fermentation medium for fermentation and culture , the microbial fermentation inoculant is obtained after the fermentation is finished.

[0030] Paenibacillus polymyxa (Paenibacillus polymyxa) is a kind of spore-forming Gram-positive bacteria, which has both disease-preventing and growth-promoting effects on plants without pathogenicity. Most strains of Paenibacillus polymyxa tested under anaerobic conditions can fix nitrogen, and can prevent and control a variety of soil-borne diseases and foliar diseases caused by pathogenic bacteria belonging to 2 genera of Oomycota, 24 genera of Fungi and 6 genera of Bacteria. disease. Polymyxins produced by Paenibacillus polymyxa disrupt the permeability of the plasma membrane of Gram-...

Embodiment 1

[0070] The screening of embodiment 1 microbial strain agent

[0071] By carrying out the screening experiment of nitrogen fixation, phosphorus and potassium decomposition on the existing bacterial strains of the applicant, the nitrogen fixation, phosphorus and potassium decomposing strains are screened out.

[0072] 1.1 Preliminary screening of nitrogen-fixing bacteria

[0073] The initial screening of nitrogen-fixing bacteria adopts the growth rate method. The isolated nitrogen-fixing bacteria are activated on nutrient agar medium, and then a ring of activated bacteria is transplanted into a test tube (18*180mm) containing Ashby liquid medium with an inoculation loop. The tube was filled with 5 mL of culture solution, and the control was the culture solution without strains, and repeated 3 times. 30°C, 120r / min shaking culture for 36h, measure the OD of each bacterial solution 600 value, and according to OD 600 The value size selects the strain.

[0074] 1.2 Re-screening ...

Embodiment 2

[0100] Antagonism test of embodiment 2 bacterial strain

[0101] Inoculate the activated phosphate solubilizing bacteria, potassium solubilizing bacteria and nitrogen fixing bacteria into sterilized liquid medium (100mL / 250mL) respectively, culture them in a 30°C incubator for 5 days, absorb the fermentation broth and observe the growth of the strains under a high power microscope Are there any exceptions. Test result shows, each single bacterial strain grows well and does not have rejection phenomenon, can symbiosis, does not take place non-antagonistic reaction ( Figure 5 ), indicating that these three bacteria can be mixed cultured. The composition of the liquid culture medium mentioned above is composed of 0.5% of beef extract powder, 1% of peptone, 0.5% of sodium chloride, 1.3-20% of agar, and pH 7.0.

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Abstract

The invention relates to the field of microbial fertilizers, in particular to a high-nitrogen compound microbial fertilizer as well as a preparation method and application of the high-nitrogen compound microbial fertilizer. Thehigh-nitrogen compound microbial fertilizer comprises amicrobial fermentation inoculant and potassium fertilizer. The microbial fermentation inoculant is prepared by the following steps: inoculating paenibacillus polymyxa, bacillus subtilis and paenibacillus mucilaginosus intoa microbial fermentation culture medium for fermentation culture, and obtaining the microbial fermentation inoculant after fermentation is finished, the microbial fermentation culture medium comprises the following substances in percentage by mass: 50-80g / L of urea; 210-300 g / L of soybean meal; 30-50 g / L of corn flour; 0.5-30 g / L of calcium chloride; 3-5 g / L of dipotassium phosphate; 0.1 to 10g / L of magnesium sulfate; 0.1 to 10 g / L of manganese sulfate; and water. According to the high-nitrogen compound microbial fertilizer disclosed by the invention, mixed fermentation of various microorganisms does not have an antagonism effect, the activity of a single strain can be obviously improved, the fermentation period is shortened to 2d, and meanwhile, the conversion rate of urea and soybean meal is increased.

Description

technical field [0001] The invention relates to the field of microbial fertilizers, in particular to a high-nitrogen compound microbial fertilizer and its preparation method and application. Background technique [0002] Urea, also known as urea, carbonamide or carbonamide, is synthesized from ammonia and carbon dioxide under high pressure and is the most commonly used dehydrated nitrogen fertilizer product. Urea is the first neutral organic compound synthesized. It exists in the soil in a molecular state after being applied to the soil, but its utilization rate is low, and only a small part of nitrogen is absorbed by the soil colloid. Urea is converted into ammonium carbonate by the action of urease in the soil to form NH4 + -N, its fate is the same as ammonium nitrogen fertilizer. At present, the fertilizer efficiency of urea in my country is generally 20% to 35%, usually lower than 30%. One of the important reasons is that too much or too much activity of urease in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/01C12R1/125
CPCC12N1/20C05F11/08
Inventor 郝祥蕊吴小珍杨亮亮丁千岭张红艳冯镇泰
Owner SHANGHAI NONGLE BIOLOGICAL PROD
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