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Application of bioactive peptide in high-nutrition dairy product and preparation method of bioactive peptide

A technology of bioactive peptides and dairy products, which is applied in the fields of food and biology, and can solve the problems of bioactive peptide stability, bioactivity, and compound functional bioactive peptides to be developed, so as to inhibit growth and reproduction, improve The body's immune microenvironment and the effect of improving the body's immunity

Inactive Publication Date: 2022-03-22
北京瞳石生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The bioactive peptides derived from milk have rich physiological functions, so they are widely used in the fields of food, medicine and health products. The stability of active peptides needs to be improved, and how to apply it in modern food engineering, improve food quality and nutritional value, and promote the absorption and utilization of nutrients in the body and other technical issues

Method used

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  • Application of bioactive peptide in high-nutrition dairy product and preparation method of bioactive peptide
  • Application of bioactive peptide in high-nutrition dairy product and preparation method of bioactive peptide
  • Application of bioactive peptide in high-nutrition dairy product and preparation method of bioactive peptide

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Effect test

Embodiment 1

[0020] Embodiment 1 bovine colostrum protein hydrolysis

[0021] Studies have shown that bovine colostrum is rich in nutrients, and the protein content, including immunoglobulin, lactoferrin, and growth factors, is much higher than that of late-stage milk, especially the whey protein content can be more than 5 times higher than that of late-stage milk. In the invention, bovine colostrum is selected for hydrolysis to extract biologically active peptides, so as to obtain novel peptide substances with excellent properties.

[0022] Take the fresh bovine colostrum secreted by the cow within 2-3 days after the calf was born, add 95% ethanol to wash, and centrifuge at 2000r / min for 15 minutes to obtain skim milk; add phosphate buffer to prevent protein denaturation caused by drastic pH changes; add papaya Protease (300u / mL), bromelain (300u / mL), elastase (500u / mL), trypsin (500u / mL), put them in a 42°C water bath for 4-5h; put the reaction solution in boiling water 10min in the dom...

Embodiment 2

[0023] Embodiment 2 bioactive peptide obtains

[0024] In the present invention, a reversed-phase high performance liquid phase-mass spectrometry method is used to separate bovine milk bioactive peptides. The above biologically active peptide sample is diluted with a mobile phase as a loading sample, and the loading sample is analyzed by reversed-phase high performance liquid phase-mass spectrometry. Set the mobile phase system to 0.1% trifluoroacetic acid / methanol / water system, the volume fraction of methanol is set to 20%, 10%, 8%, and 5%, and the corresponding flow rates are 1.0, 0.8, 0.5, and 0.3mL / min. The injection volume was 10 μL, the column temperature was 30°C, the temperature of the injection chamber was 4°C, and the test time was 30 minutes for full-wavelength ultraviolet scanning. Then, replace trifluoroacetic acid with phosphate buffer in the mobile phase of 0.1% trifluoroacetic acid / 6% methanol / water system, adjust the pH of the phosphate buffer to 6.0, and per...

Embodiment 3

[0026] Example 3 Detection of Bioactive Peptide Antioxidant Ability

[0027] Active oxygen free radicals can cause lipid peroxidation in the body, accelerate the aging process of the entire body from the skin to internal organs, and induce skin lesions, cardiovascular diseases, cancer, etc., seriously endangering human health. Adopt the pyrogallol method to measure the scavenging capacity of the bioactive peptide to the superoxide anion free radical among the present invention, get the Tris-HCI damping fluid of 4.5mL 0.5mL / L respectively, the EDTA buffer solution of 1.0mL (9mol / L), add 1.0mL of active peptide samples of different concentrations, add 1.0mL of distilled water to the blank group, vortex disperser shake, put in a water bath at 25°C for 10min, add 0.05mL of pyrogallol solution (0.45mol / L), shake After 3 minutes, add 0.05 mL of ascorbic acid solution (50 μg / mL), shake well, and measure the absorbance at a wavelength of 325 nm.

[0028] Superoxide anion radical sca...

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Abstract

The invention relates to application of a bioactive peptide in a high-nutrition dairy product and a preparation method of the bioactive peptide. According to the invention, bovine coloctrum is hydrolyzed by adopting various proteases, and a bioactive peptide with a brand new amino acid structure is screened and obtained, and the peptide can remove oxygen free radicals, promote proliferation of immune cells including lymphocytes, inhibit growth and reproduction of harmful microorganisms, induce secretion of immune factors in vivo and improve the immunity of the organism. Besides, the invention further provides a nano preparation delivery system, the active peptide can be used in an oral mode, high bioavailability of the active peptide is kept, and convenience is provided for development of follow-up related dairy products.

Description

technical field [0001] The invention belongs to the field of food and biotechnology, and in particular relates to the application of bioactive peptides in high-nutrition dairy products and a preparation method thereof. Background technique [0002] Bioactive peptides are generally composed of 2-30 amino acids and have specific biological functions. There are reports on plant proteins, seafood proteins, meat proteins, milk proteins, and legume proteins. The physiological functions of bioactive peptides mainly focus on antithrombotic, hypotensive, antioxidative, osteogenic, antitumor, immune regulation, anti-inflammatory, lipid-lowering, antibacterial, and hypoglycemic. [0003] Milk contains important nutrients, immune protection substances and bioactive components required by humans, making it an important source of bioactive peptides. Generally speaking, the main protein components in milk include: α-LA, β-LG, casein, immunoglobulin, lactoferrin, protein peptide components...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47
CPCC07K14/47C12P21/06A23C9/1322A23C9/1526A23C19/0921B82Y5/00
Inventor 张冬久王京
Owner 北京瞳石生物科技有限公司
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