The invention discloses a spleen-tonifying, qi-supplementing and cancer-resisting hot dog sausage and a preparation method thereof. The spleen-tonifying, qi-supplementing and cancer-resisting hot dog sausage comprises the following raw materials in parts by weight: medlar, ginseng, poria cocos, astragalus, atractylodes macrocephala koidz, chicken breast, lean, pork fat, separation proteins, modified starches, carrageenan, iced water, edible salt, white sugar, aginomoto, chicken essence, white fungus, pork essence, monascus color, allura red, composite phosphate, poria cocos, sodium nitrite, white pepper powder, anise powder and matured oyster mushroom. The spleen-tonifying, qi-supplementing and cancer-resisting hot dog sausage prepared by the preparation method disclosed by the invention can relieve infantile anorexia and is high in edible efficiency and nutrition value.