The invention discloses a multifunctional cross infection prevention body fluid collection and inspection device which comprises an end holder, a collecting tube, a pushing part and a fluid storage part. The collecting tube and the pushing part are detachably arranged on the end holder, the pushing part can be inserted into the collecting tube, the pushing part comprises a mounting tube and a pushrod, a fluid guide plate is arranged in the mounting tube, the push rod is arranged on the fluid guide plate, a sealing part is arranged at an outlet end of the fluid storage part, can penetrate theend holder and is inserted into the mounting tube, the sealing part comprises a sealing block, an annular tube and an inverted U-shaped cover, the annular tube is arranged on the sealing block, and the inverted U-shaped cover externally covers the annular tube and is hermetically connected with the sealing block. When the fluid storage part is inserted into the mounting tube, the push rod pushes the inverted U-shaped cover upwards, so that the inverted U-shaped cover is separated from the sealing block to form a fluid passage gap. A patient can be effectively prevented from contacting with body fluids in fluid extraction, inspection staff can be effectively prevented from contacting with the body fluids in the inspection process, cross infection is avoided, air pollution caused by undesirable odor emission can be avoided, and the device has the functions of a holder.