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Fat coated biological enzyme or micro-ecological preparation

A technology of micro-ecological preparations and biological enzymes, applied in animal feed, animal feed, applications, etc., can solve the problems of easy loss of biological activity, large loss of activity, slow growth rate, etc., to achieve adjustable coating thickness and reduce inactivation , the effect of uniform release

Active Publication Date: 2014-06-25
王宏雁 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problems to be solved urgently with probiotics as feed additives are: ①Live bacteria preparations tend to lose biological activity during feed processing, transportation and storage. These microorganisms are especially sensitive to high temperature. When the pelleting temperature exceeds 85°C, the activity is almost lost. ;② After the live bacteria preparation enters the digestive tract, it cannot withstand the action of low pH hydrochloric acid and bile acid, and it is difficult to have enough viable bacteria to reach the intestinal tract or colonize the intestinal tract to play a role (generally, the concentration of viable bacteria in the microecological preparation is required for 10 7 cfu / mL); ③Live bacterial preparations grow slowly after entering the intestinal tract, and it is difficult to be in a dominant position in microbial competition and form a dominant flora. It is often difficult to achieve the desired effect under the conditions
However, the disadvantage of spray drying is that the hot air temperature is high, the activity loss is large during processing, and the processing cost is also high. The preparation of microcapsules by chemical methods can be carried out at a lower temperature, such as using sodium alginate and calcium chloride to stimulate the digestive chain. Coccus and Lactobacillus are coated, which significantly prolongs the survival period of Lactobacillus, but the preparation reaction is carried out in an aqueous solution, and the product drying also requires a higher temperature; since the above methods are all produced in an air atmosphere, the Oxygen can lead to the death of anaerobic microorganisms such as bifidobacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The coating material is prepared with 60% stearic acid glyceryl succinate polyester (melting point 89°C), 30% hydrogenated vegetable oil (melting point 50°C), and 10% by weight of polymerized glycerin fatty acid ester.

[0025] Such as figure 1 As shown, add 350kg of the coating material prepared according to the above ratio into the jacketed dissolution kettle 1 (with agitator or ultrasonic generator, pressure P≥2MPa), seal it, and evacuate until the dissolution kettle vacuum is shown in the table The pressure is 0.09MPa, open the hydrocarbon pump 3 and inject 2000L liquefied petroleum gas (or other solvents) (compressed in advance, cooled to liquid and stored in the storage tank 4), stir, pass hot water in the jacket to help dissolve, and dissolve in the kettle 1 The temperature of the solution containing the coating material is below 35°C.

[0026] 150kg cellulase (enzyme activity 1×10 4 U / g) is placed in the encapsulation tank (using rake dryer 2), and the solutio...

Embodiment 2

[0028] The coating material is prepared with 50% of palm stearin, 40% of glyceryl adipate stearate, and 10% by weight of polymerized glycerin fatty acid ester.

[0029] Such as figure 2 As shown, add 200kg of the coating material prepared according to the above ratio to the dissolution kettle 1 (with agitator or ultrasonic generator, pressure P≥2MPa), seal it, and vacuum until the vacuum of the dissolution kettle is 0.09MPa. , turn on the hydrocarbon pump 3 and inject 1000L butane (or other solvents) (compressed in advance, cooled to a liquid and stored in the storage tank 4), stir, the jacket passes hot water to help dissolve, and the solution temperature in the dissolution tank 1 is lower than 35°C , stop the hot water supply after the material liquid in the dissolving tank becomes transparent.

[0030] In encapsulation kettle 2, add 300kg lipase (enzyme activity 1 * 10 5 U / g), vacuumize until the vacuum indication number of the encapsulation kettle 2 is 0.09MPa, open the...

Embodiment 3

[0032] The coating material is prepared with 80% hydrogenated vegetable oil, 10% glycerol monostearate and 10% sucrose ester by weight.

[0033] Such as figure 2 As shown, add 100kg of the coating material prepared by the above ratio into the dissolution tank 1 (with agitator or ultrasonic generator, pressure P≥2MPa), seal it, and vacuumize until the gauge pressure of the dissolution tank is 0.09MPa. , turn on the hydrocarbon pump 3 and inject 1000L butane (or other solvent) solution (compressed in advance, cooled to liquid and then stored in the storage tank 4), stirring, the jacket is fed with hot water to help dissolve, and the coating material is dissolved in the kettle 1 The temperature of the solution is lower than 35°C, and the hot water supply is stopped after the liquid in the kettle becomes transparent.

[0034] Add 400kg bifidobacteria-lactic acid bacteria solid powder in encapsulation kettle 2 (viable bacteria quantity 2 * 10 8 unit / g), vacuumize until the gauge p...

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PUM

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Abstract

The invention discloses a fat coated biological enzyme or micro-ecological preparation. A preparation method of the fat coated biological enzyme or micro-ecological preparation comprises the steps of firstly, compressing solvent liquefied petroleum gas and the like which are in a gas state under the conditions of normal pressure and room temperature to obtain liquid, and storing the liquid into a storage tank; putting a coating material into a dissolving kettle, vacuumizing, injecting the liquid solvent in the storage tank into the dissolving kettle by a hydrocarbon pump, and heating the liquid solvent until the liquid solvent becomes subcritical fluid; stirring to dissolve the coating material; feeding an enzyme preparation or micro-ecological preparation into a packaging kettle, sealing and then vacuumizing; opening the hydrocarbon pump and injecting the solution containing the coating material in the dissolving kettle into the packaging kettle; reducing the pressure for releasing the solvent; controlling the temperature of the material in the packaging kettle not to exceed 35 DEG C; when the pressure in the packaging kettle becomes normal pressure, vacuumizing and emptying the packaging kettle to enable the pressure in the packaging kettle to become normal pressure; and discharging to obtain the coated enzyme preparation or micro-ecological preparation finished product. The preparation method has the advantages that the whole process can be completed under the conditions of being close to room temperature and free of oxygen, so that the inactivation of the enzyme preparation or the micro-ecological preparation can be greatly alleviated in the processing process, and the product activity is improved.

Description

technical field [0001] The invention relates to a feed additive---a biological enzyme and a microecological preparation, in particular to a fat-coated biological enzyme and a microecological preparation. Background technique [0002] Enzyme is a protein with catalytic activity. It has high efficiency and specificity. It exists widely in animals, plants and microorganisms. It is an essential component for organisms to maintain normal physiological and biochemical functions. The utilization of nutrients in feed by poultry and livestock is to degrade various macromolecular substances into small molecular substances under the action of various enzymes in the digestive tract and then absorb and utilize them. The digestion and absorption capacity of nutrients in animal feed depends on the type and activity of enzymes in the digestive tract. However, the endogenous enzymes secreted in the digestive tract of livestock and poultry to degrade feed ingredients are often lacking and in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/165
Inventor 王宏雁曹胜炎
Owner 王宏雁