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Soil heavy metal repairing agent, preparation thereof and application

A technology of heavy metals and remediation agents, applied in applications, soil conditioning materials, other chemical processes, etc., can solve the problems of low stability of the remediation effect of a single functional bacteria, narrow tolerance range of heavy metal cadmium, and inability to promote microbial proliferation, etc., to achieve The effect of increasing yield is remarkable, which is beneficial to passivation and the effect of improving soil acidification

Active Publication Date: 2015-12-02
FUJIAN SANJU BIOLOGICAL SCI & TECH
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  • Abstract
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Problems solved by technology

[0003] Phytoremediation is limited by the biomass of hyperaccumulating plants, and the remediation time is longer
Organic and inorganic chemical passivation remediation can easily cause certain damage to the soil environment. While passivating heavy metals, it is easy to reduce the activity of nutrient elements in the soil. Microbial remediation is widely used because of its low cost, environmental friendliness and improvement of soil fertility. Chinese patent 201410101334.8 provides a cadmium-tolerant bacterium and its method for inhibiting the absorption of heavy metal cadmium in rice, and discloses a method for repairing cadmium pollution in rice fields with Pseudomonas liquid. It has the disadvantage of low microbial activity, and the stability of the repair effect of a single functional bacteria is low
Chinese patent 201410681387.1 provides a microbial remediation agent for cadmium-contaminated soil and its application. It only simply adsorbs microorganisms on solid substrates such as bentonite and sepiolite, but cannot promote the proliferation of microorganisms, and combines microorganisms with nutrients nitrogen, phosphorus, zinc, Direct compounding of iron is easy to reduce microbial activity
Chinese patent 201410829632.9 provides a microbial composite bacterial agent for soil remediation and its preparation method and use. The composite bacterial agent is composed of a certain proportion of photosynthetic bacteria, Lactobacillus bulgaricus, Bacillus subtilis, and Saccharomyces cerevisiae, and has a tolerance range for heavy metal cadmium Narrow, the application method is spreading and spraying on the soil surface, and it is also easy to cause the biological activity of the fungicide to decrease

Method used

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  • Soil heavy metal repairing agent, preparation thereof and application
  • Soil heavy metal repairing agent, preparation thereof and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, bacterial strain and soil heavy metal remediation agent are to Cd 2+ The dynamic adsorption effect of

[0036] Secondary fermentation of Issaccharomyces orientalis, Bacillus licheniformis and Paecilomyces lilacinus were inoculated into Cd 2+ Concentration (g / L) in the fermentation medium of 0.2, 0.4, 0.6, 0.8, 1.0, at 28 ℃, 180rpm shaking culture 24h, sampling 6000rpm centrifugation 10min, take the supernatant and use atomic spectrophotometry to measure the fermentation broth and control The content of heavy metal cadmium in the medium (without inoculating strains) was used to calculate the removal rate.

[0037] Issaccharomyces orientalis, Bacillus licheniformis, Paecilomyces lilacinus contain Cd 2+ In the fermentation broth with a concentration of 0.2-1g / L, after 24 hours of fermentation, the removal rate of cadmium is more than or equal to 50%. In the 0.4g / L cadmium solution, the removal rate of I. orientalis to cadmium can reach up to 74.25%, the re...

Embodiment 2

[0043] Embodiment 2, the restoration effect of soil heavy metal remediation agent to different degrees of cadmium polluted soil

[0044] Adopt 0.3mg / kg (treatment 1), 3mg / kg (treatment 2), 15mg / kg (treatment 3) self-made cadmium contaminated soil to carry out soil culture test, each treatment experiment is set up two groups, one group is control group (not using Soil heavy metal remediation agent), another group is the experimental group (application of soil heavy metal remediation agent), and each group of experiments is repeated three times. The prepared soil heavy metal remediation agent was added to the cadmium-contaminated soil, mixed well, the soils of different treatments were quantitatively weighed and placed in pots for cultivation, watered regularly, and the soil humidity was kept to 60% of the field water capacity. Samples were taken at 0d, 15d, and 40d. After the soil samples were air-dried, the available cadmium content in the soil was detected.

[0045] Table 2 ...

Embodiment 3

[0050] Embodiment 3, pot test application effect of soil heavy metal remediation agent

[0051] 1 test treatment

[0052] The test plant was lettuce, and the seedlings with the same growth were selected for the pot experiment. The pot soil was self-made cadmium-contaminated soil. The experiment consisted of 8 treatments, and the difference in the application amount of the bacterial solution between the treatments was made up with sterilized medium, and repeated 5 times. times, 5-6 strains per pot, and the experimental treatment design is shown in Table 4.

[0053] Table 4 pot experiment treatment design of lettuce

[0054]

[0055] Note: Paecilomyces lilacinus, Issaccharomyces orientalis, and Bacillus licheniformis are referred to as Lavender, Lichen, and Orient respectively.

[0056] 2 Results and Analysis

[0057] Each treatment was placed in a greenhouse with the functions of shelter from rain, ventilation, and temperature adjustment, and uniform daily spray watering ...

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Abstract

The invention provides a soil heavy metal repairing agent, a preparation thereof and application, wherein the soil heavy metal repairing agent comprises paecilomyces lilacinus, Issatchenkia orientalis, Bacillus licheniformis, and organic active carriers with quite strong heavy metal adsorption function. The preparation method comprises mixing the functional bacterial strains fermentation broth and the organic active carriers uniformly in proportion to get the soil heavy metal repairing agent. The soil heavy metal repairing agent in the invention has not less than 0.2 billion / g of effective live bacteria number, not less than 55% of organic matter content, and the water content is in the range of 20% to 25%, the value of pH is in the range of 8 to 8.5. The optimal application method comprises broadcasting the soil heavy metal repairing agents in an amount of 300 kg / mu, plowing and mixing uniformly with the soil, and balancing the paddy field for 30 days. The soil heavy metal repairing agent has low cost, good effect, and can in-situ rehabilitate on soil heavy metal contamination, which suits our national condition of cultivated land resource shortage and has important economy value and ecological significance on farmland heavy metal control.

Description

technical field [0001] The invention belongs to the technical field of bioremediation application of soil heavy metals, and in particular relates to the preparation and application of a cadmium pollution remediation agent. Background technique [0002] Heavy metal cadmium and its compounds are toxic to a certain extent, and were once the source of pollution that caused Japan's "Itai-Itai" pollution incident. In February 2013, Nanfang Daily published the article "Hunan Problem Rice Flows to Guangdong's Table". The pollution of cultivated land soil by heavy metal cadmium has attracted widespread attention. According to the National Soil Pollution Survey Bulletin, among the 8 heavy metal pollutants of cadmium, mercury, arsenic, copper, lead, chromium, zinc, and nickel, cadmium pollution ranks first, and its point exceeding standard rate reaches 7%. Cadmium is the primary pollutant in cultivated land. Cadmium pollution mainly exists in mining areas, sewage irrigation areas, and...

Claims

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Application Information

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IPC IPC(8): C09K17/00A01B79/02C09K101/00C09K109/00
Inventor 余劲聪林克明
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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